cDNA Clone FASCIN1

• Plasmid: pCMV5-HA FASCIN1
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-FASCIN1
• Genbank: NM_003088
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGACCGCCAACGGCACAGCCGAGGCGGTGCAGATCCAGTTCGG
CCTCATCAACTGCGGCAACAAGTACCTGACGGCCGAGGCGTTCGGGTTCA
AGGTGAACGCGTCCGCCAGCAGCCTGAAGAAGAAGCAGATCTGGACGCTG
GAGCAGCCCCCTGACGAGGCGGGCAGCGCGGCCGTGTGCCTGCGCAGCCA
CCTGGGCCGCTACCTGGCGGCGGACAAGGACGGCAACGTGACCTGCGAGC
GCGAGGTGCCCGGTCCCGACTGCCGTTTCCTCATCGTGGCGCACGACGAC
GGTCGCTGGTCGCTGCAGTCCGAGGCGCACCGGCGCTACTTCGGCGGCAC
CGAGGACCGCCTGTCCTGCTTCGCGCAGACGGTGTCCCCCGCCGAGAAGT
GGAGCGTGCACATCGCCATGCACCCTCAGGTCAACATCTACAGCGTCACC
CGTAAGCGCTACGCGCACCTGAGCGCGCGGCCGGCCGACGAGATCGCCGT
GGACCGCGACGTGCCCTGGGGCGTCGACTCGCTCATCACCCTCGCCTTCC
AGGACCAGCGCTACAGCGTGCAGACCGCCGACCACCGCTTCCTGCGCCAC
GACGGGCGCCTGGTGGCGCGCCCCGAGCCGGCCACTGGCTACACGCTGGA
GTTCCGCTCCGGCAAGGTGGCCTTCCGCGACTGCGAGGGCCGTTACCTGG
CGCCGTCGGGGCCCAGCGGCACGCTCAAGGCGGGCAAGGCCACCAAGGTG
GGCAAGGACGAGCTCTTTGCTCTGGAGCAGAGCTGCGCCCAGGTCGTGCT
GCAGGCGGCCAACGAGAGGAACGTGTCCACGCGCCAGGGTATGGACCTGT
CTGCCAATCAGGACGAGGAGACCGACCAGGAGACCTTCCAGCTGGAGATC
GACCGCGACACCAAAAAGTGTGCCTTCCGTACCCACACGGGCAAGTACTG
GACGCTGACGGCCACCGGGGGCGTGCAGTCCACCGCCTCCAGCAAGAATG
CCAGCTGCTACTTTGACATCGAGTGGCGTGACCGGCGCATCACACTGAGG
GCGTCCAATGGCAAGTTTGTGACCTCCAAGAAGAATGGGCAGCTGGCCGC
CTCGGTGGAGACAGCAGGGGACTCAGAGCTCTTCCTCATGAAGCTCATCA
ACCGCCCCATCATCGTGTTCCGCGGGGGGCATGGCTTCATCGGCTGCCGC
AAGGTCACGGGCACCCTGGACGCCAACCGCTCCAGCTATGACGTCTTCCA
GCTGGAGTTCAACGATGGCGCCTACAACATCAAAGACTCCACAGGCAAAT
ACTGGACGGTGGGCAGTGACTCCGTGGTCACCAGCAGCGGCGACACTCCT
GTGGACTTCTTCTTCGAGTTCTGCGACTATAACAAGGTGGCCATCAAGGT
GGGCGGGCGCTACCTGAAGGGCGACCACGCAGGCGTCCTGAAGGCCTCGG
CGGAAACCGTGGACCCCGCCTCGCTCTGGGAGTACTAGgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASAS
SLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPD
CRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAM
HPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSV
QTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSG
TLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEE
TDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDI
EWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVF
RGGHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSD
SVVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPA
SLWEY

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7031
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express human FASCIN1 homolog with N-terminal HA tag.; Cloned protein sequence has a E391G change compared to the published NM_003088 sequence.