cDNA Clone FASCIN1

• Plasmid: pGEX6P-FASCIN1
• Parent Plasmid: pGEX6P1
• Expressed: GST-FASCIN1
• Genbank: NM_003088
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGACCGCCAACGGCACAGCCGAGGCGGTGCAGATCCAGTTCGG
CCTCATCAACTGCGGCAACAAGTACCTGACGGCCGAGGCGTTCGGGTTCA
AGGTGAACGCGTCCGCCAGCAGCCTGAAGAAGAAGCAGATCTGGACGCTG
GAGCAGCCCCCTGACGAGGCGGGCAGCGCGGCCGTGTGCCTGCGCAGCCA
CCTGGGCCGCTACCTGGCGGCGGACAAGGACGGCAACGTGACCTGCGAGC
GCGAGGTGCCCGGTCCCGACTGCCGTTTCCTCATCGTGGCGCACGACGAC
GGTCGCTGGTCGCTGCAGTCCGAGGCGCACCGGCGCTACTTCGGCGGCAC
CGAGGACCGCCTGTCCTGCTTCGCGCAGACGGTGTCCCCCGCCGAGAAGT
GGAGCGTGCACATCGCCATGCACCCTCAGGTCAACATCTACAGCGTCACC
CGTAAGCGCTACGCGCACCTGAGCGCGCGGCCGGCCGACGAGATCGCCGT
GGACCGCGACGTGCCCTGGGGCGTCGACTCGCTCATCACCCTCGCCTTCC
AGGACCAGCGCTACAGCGTGCAGACCGCCGACCACCGCTTCCTGCGCCAC
GACGGGCGCCTGGTGGCGCGCCCCGAGCCGGCCACTGGCTACACGCTGGA
GTTCCGCTCCGGCAAGGTGGCCTTCCGCGACTGCGAGGGCCGTTACCTGG
CGCCGTCGGGGCCCAGCGGCACGCTCAAGGCGGGCAAGGCCACCAAGGTG
GGCAAGGACGAGCTCTTTGCTCTGGAGCAGAGCTGCGCCCAGGTCGTGCT
GCAGGCGGCCAACGAGAGGAACGTGTCCACGCGCCAGGGTATGGACCTGT
CTGCCAATCAGGACGAGGAGACCGACCAGGAGACCTTCCAGCTGGAGATC
GACCGCGACACCAAAAAGTGTGCCTTCCGTACCCACACGGGCAAGTACTG
GACGCTGACGGCCACCGGGGGCGTGCAGTCCACCGCCTCCAGCAAGAATG
CCAGCTGCTACTTTGACATCGAGTGGCGTGACCGGCGCATCACACTGAGG
GCGTCCAATGGCAAGTTTGTGACCTCCAAGAAGAATGGGCAGCTGGCCGC
CTCGGTGGAGACAGCAGGGGACTCAGAGCTCTTCCTCATGAAGCTCATCA
ACCGCCCCATCATCGTGTTCCGCGGGGGGCATGGCTTCATCGGCTGCCGC
AAGGTCACGGGCACCCTGGACGCCAACCGCTCCAGCTATGACGTCTTCCA
GCTGGAGTTCAACGATGGCGCCTACAACATCAAAGACTCCACAGGCAAAT
ACTGGACGGTGGGCAGTGACTCCGTGGTCACCAGCAGCGGCGACACTCCT
GTGGACTTCTTCTTCGAGTTCTGCGACTATAACAAGGTGGCCATCAAGGT
GGGCGGGCGCTACCTGAAGGGCGACCACGCAGGCGTCCTGAAGGCCTCGG
CGGAAACCGTGGACCCCGCCTCGCTCTGGGAGTACTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTANGTAEAVQIQFGLINC
GNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRY
LAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRL
SCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDV
PWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSG
KVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAAN
ERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTA
TGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVET
AGDSELFLMKLINRPIIVFRGGHGFIGCRKVTGTLDANRSSYDVFQLEFN
DGAYNIKDSTGKYWTVGSDSVVTSSGDTPVDFFFEFCDYNKVAIKVGGRY
LKGDHAGVLKASAETVDPASLWEY

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU7030
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloned protein sequence has a E391G change compared to the published NM_003088 sequence.; ; Estimated MW of expressed protein : 81306 Daltons