cDNA Clone PIK4CA

• Plasmid: pFBHTa PIK4CA
• Parent Plasmid: pSC-A
• Expressed: 6H-PIK4CA
• Genbank: NM_058004.2
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGTGCCCAGTAGATTTTCATGGGATCTTCCAGTTAGATGAAAG
ACGGAGAGATGCAGTGATTGCATTGGGCATTTTTCTGATTGAATCTGATC
TTCAGCACAAAGATTGTGTGGTTCCTTACCTTCTTCGACTTCTCAAAGGT
CTTCCAAAAGTGTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGG
TGCCCTCCCGGTTGCAGAGAGCTTCAGCTTCTGCTTGGTAACTCTGCTGT
CTGATGTGGCCTATAGGGATCCTTCACTTAGGGATGAGATTTTAGAGGTG
CTTTTGCAGGTTTTGCATGTCCTCTTGGGGATGTGCCAGGCCTTGGAGAT
TCAAGACAAAGAATACCTTTGCAAGTATGCTATCCCATGCCTGATAGGAA
TCTCGCGAGCATTTGGGCGTTACAGCAACATGGAAGAGTCTCTCCTCTCA
AAGCTCTTTCCCAAAATCCCTCCTCATTCCCTCCGTGTCCTGGAAGAGCT
TGAAGGTGTTCGAAGGCGTTCCTTTAATGACTTCCGCTCCATCCTCCCCA
GCAATCTGCTGACTGTCTGTCAGGAGGGTACCCTGAAGAGGAAAACCAGC
AGTGTGTCCAGCATCTCTCAGGTCAGCCCTGAACGCGGCATGCCCCCTCC
CAGTTCCCCTGGAGGATCTGCCTTTCACTACTTTGAAGCCTCCTGTTTGC
CCGATGGGACTGCCCTAGAGCCTGAGTACTACTTTTCAACCATCAGCTCC
AGCTTCTCAGTCTCTCCCCTTTTCAACGGTGTCACATATAAGGAGTTTAA
CATTCCATTGGAAATGCTTCGGGAACTCTTAAACCTGGTGAAGAAGATCG
TTGAGGAGGCTGTTCTCAAATCTTTGGATGCCATTGTAGCCAGTGTGATG
GAGGCCAACCCCAGTGCTGATCTCTACTACACTTCCTTCAGTGACCCTCT
CTACCTGACCATGTTCAAGATGCTGCGTGACACTCTGTACTACATGAAGG
ACCTCCCGACCTCTTTTGTGAAGGAGATCCATGATTTTGTGCTGGAGCAG
TTCAACACGAGCCAGGGGGAGCTCCAGAAGATTCTACATGACGCAGACCG
GATCCACAATGAGCTGAGCCCCCTCAAACTGCGCTGTCAGGCGAGTGCTG
CCTGTGTGGACCTCATGGTGTGGGCTGTGAAGGACGAGCAGGGTGCAGAA
AACCTTTGCATCAAGCTATCTGAGAAGCTGCAGTCCAAGACGTCCAGCAA
AGTCATTATTGCTCACTTGCCCCTGCTGATCTGCTGTCTGCAGGGTTTGG
GCCGCCTGTGCGAGAGGTTCCCGGTGGTGGTGCACTCTGTGACACCGTCC
TTGCGAGACTTCCTGGTCATCCCGTCCCCAGTTCTGGTGAAGCTCTACAA
GTACCACAGTCAGTACCACACAGTTGCTGGCAATGATATAAAAATCAGTG
TGACCAATGAGCATTCCGAGTCAACCCTGAACGTCATGTCGGGTAAGAAG
AGCCAGCCCTCCATGTACGAGCAGCTCCGAGACATCGCTATTGACAACAT
CTGCAGGTGCCTGAAGGCTGGATTGACGGTGGACCCAGTGATTGTGGAGG
CGTTCTTGGCCAGCCTGTCCAACCGGCTCTACATCTCTCAGGAGAGCGAC
AAGGACGCTCACTTGATTCCCGACCACACAATCCGAGCCTTGGGACACAT
TGCGGTGGCCTTGAGGGACACCCCGAAGGTCATGGAGCCCATTCTGCAGA
TCCTACAGCAGAAATTCTGCCAGCCACCCTCCCCCCTCGATGTGCTGATT
ATTGACCAGCTGGGCTGCCTGGTCATCACCGGAAATCAATACATCTATCA
GGAAGTGTGGAACCTCTTCCAGCAGATCAGTGTGAAGGCCAGCTCCGTTG
TATACTCAGCCACCAAAGATTACAAGGACCACGGCTATAGGCATTGCTCC
CTGGCAGTGATTAATGCCCTGGCCAACATCGCGGCCAACATCCAAGACGA
GCACCTGGTGGATGAGCTGCTCATGAACCTGTTGGAGTTGTTTGTGCAGC
TGGGGCTGGAGGGGAAGCGAGCCAGCGAGAGGGCAAGCGAGAAGGGCCCT
GCCCTAAAGGCTTCTAGCAGTGCAGGGAACTTGGGAGTACTCATTCCTGT
AATAGCTGTGCTCACCCGACGACTGCCACCCATCAAAGAAGCTAAGCCTC
GGTTACAGAAGCTCTTCCGAGACTTCTGGCTGTATTCCGTTCTGATGGGA
TTCGCTGTGGAGGGCTCAGGACTCTGGCCAGAAGAATGGTACGAGGGGGT
CTGTGAAATAGCCACTAAGTCCCCCTTGCTCACCTTTCCCAGCAAGGAGC
CACTGCGGTCCGTCCTCCAGTATAACTCAGCCATGAAGAATGACACGGTC
ACCCCCGCTGAGCTGAGTGAGCTCCGCAGCACTATCATCAACCTGCTGGA
CCCCCCTCCCGAGGTGTCCGCACTCATCAACAAGCTGGACTTCGCCATGT
CCACCTACCTCCTCTCTGTGTACCGGCTGGAGTACATGAGGGTACTGCGT
TCAACAGATCCTGATCGCTTCCAGGTAATGTTCTGCTACTTTGAGGATAA
AGCTATTCAGAAAGACAAATCTGGGATGATGCAGTGTGTGATTGCAGTCG
CGGACAAAGTATTCGATGCCTTCCTGAACATGATGGCGGATAAAGCCAAG
ACCAAGGAGAACGAGGAGGAGCTGGAGCGGCACGCTCAGTTCCTGTTGGT
GAACTTCAACCACATCCACAAGAGGATAAGGAGGGTGGCAGACAAGTATC
TATCTGGTCTGGTGGATAAGTTTCCCCACTTGCTCTGGAGCGGGACTGTG
CTGAAGACCATGCTGGACATCCTGCAGACCCTGTCACTGTCACTGAGCGC
TGATATTCACAAGGATCAGCCTTACTATGACATCCCCGACGCCCCCTACC
GGATCACGGTTCCTGACACGTACGAAGCCCGTGAGAGCATTGTGAAGGAC
TTCGCTGCACGCTGTGGGATGATCCTCCAGGAGGCCATGAAGTGGGCACC
TACCGTCACCAAGTCCCACCTGCAGGAATATCTGAACAAACATCAGAACT
GGGTATCGGGACTGTCCCAGCACACGGGGCTGGCCATGGCCACTGAGAGC
ATCCTTCACTTTGCTGGCTACAACAAGCAGAACACAACTCTTGGGGCAAC
TCAGCTGAGCGAGCGCCCGGCCTGTGTGAAGAAAGACTACTCCAACTTCA
TGGCATCCCTGAATCTGCGCAACCGCTACGCGGGCGAGGTGTATGGAATG
ATTCGGTTCTCAGGCACCACAGGCCAGATGTCTGACCTGAACAAAATGAT
GGTCCAGGATCTACATTCAGCTTTAGACCGCAGTCATCCTCAGCACTACA
CGCAGGCCATGTTCAAGCTGACCGCAATGCTCATTAGCAGTAAAGATTGT
GACCCGCAGCTCCTTCATCATCTGTGCTGGGGTCCCCTCCGGATGTTCAA
TGAGCATGGCATGGAGACGGCCCTGGCCTGCTGGGAGTGGCTGCTGGCTG
GCAAGGATGGAGTGGAAGTGCCGTTCATGCGGGAGATGGCAGGGGCCTGG
CACATGACGGTGGAGCAGAAATTTGGCCTGTTTTCTGCTGAGATAAAGGA
AGCAGACCCCCTGGCTGCCTCGGAAGCAAGTCAACCCAAACCCTGTCCCC
CCGAAGTGACCCCCCACTACATCTGGATCGACTTCCTGGTGCAGCGGTTT
GAGATCGCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCAGCCT
GCTGCAGCGCTCCATGTCCCTGAACATCGGCGGGGCCAAGGGGAGCATGA
ACCGGCACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGACCCTGGGG
CTGTCCCTCCTGCATGCCGATGTGGTTCCAAATGCAACCATCCGCAATGT
GCTTCGCGAGAAGATCTACTCCACTGCCTTTGACTACTTCAGCTGTCCCC
CAAAGTTCCCTACTCAAGGAGAGAAGCGGCTGCGTGAAGACATAAGCATC
ATGATTAAATTTTGGACCGCCATGTTCTCAGATAAGAAGTACCTGACCGC
CAGCCAGCTTGTTCCCCCAGATAATCAGGACACCCGGAGCAACCTGGACA
TAACTGTCGGCTCTCGGCAACAAGCCACCCAAGGCTGGATCAACACATAC
CCCCTGTCCAGCGGCATGTCCACCATCTCCAAGAAATCAGGCATGTCTAA
GAAAACCAACCGGGGCTCCCAGCTGCACAAATACTACATGAAGCGCAGGA
CGCTGCTGCTGTCCCTGCTGGCCACTGAGATCGAGCGTCTCATCACATGG
TACAACCCGCTGTCAGCCCCGGAACTGGAACTAGACCAGGCCGGAGAGAA
CAGCGTGGCCAACTGGAGATCTAAGTACATCAGCCTGAGTGAGAAGCAGT
GGAAGGACAACGTGAACCTCGCCTGGAGCATCTCTCCCTACCTAGCCGTG
CAGCTGCCTGCCAGGTTTAAGAACACAGAAGCCATTGGGAACGAAGTGAC
CCGTCTCGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCCTGAAGCAA
TCAAGTTCCTGGTCACCTGGCACACCATCGACGCCGATGCTCCAGAGCTC
AGCCATGTGCTGTGCTGGGCGCCCACGGACCCACCCACAGGCCTCTCCTA
CTTCTCCAGCATGTACCCGCCGCACCCTCTCACGGCGCAGTACGGGGTGA
AAGTCCTGCGGTCCTTCCCTCCGGACGCCATCCTCTTCTACATCCCCCAG
ATTGTGCAGGCCCTCAGGTACGACAAGATGGGCTATGTGCGGGAGTATAT
TCTGTGGGCAGCGTCTAAATCCCAGCTTCTGGCACACCAGTTCATCTGGA
ACATGAAGACTAACATTTATCTAGATGAAGAGGGCCACCAGAAAGACCCT
GACATCGGCGACCTCCTGGATCAGTTGGTAGAGGAGATCACAGGCTCCTT
GTCCGGCCCAGCGAAGGACTTTTACCAGCGGGAGTTTGATTTCTTTAACA
AGATCACCAACGTGTCGGCTATCATCAAGCCCTACCCTAAAGGCGACGAG
AGAAAGAAGGCTTGTCTGTCGGCCCTGTCTGAAGTGAAGGTGCAGCCGGG
CTGCTACCTGCCCAGCAACCCTGAGGCCATTGTGCTGGACATCGACTACA
AGTCTGGGACCCCGATGCAGAGTGCTGCAAAAGCCCCATATCTGGCCAAG
TTCAAGGTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAAGGTCTGCG
GTGCCGCTCAGACTCCGAGGATGAGTGCAGCACGCAGGAGGCCGACGGCC
AGAAGATCTCCTGGCAGGCAGCCATCTTCAAGGTGGGAGACGACTGCCGG
CAGGACATGCTGGCCCTGCAGATCATCGACCTCTTCAAGAACATCTTCCA
GCTGGTCGGCCTGGACCTCTTTGTTTTTCCCTACCGCGTGGTGGCCACTG
CCCCTGGGTGCGGGGTGATCGAGTGCATCCCCGACTGCACCTCCCGGGAC
CAGCTGGGCCGCCAGACAGACTTCGGCATGTACGACTACTTCACACGCCA
GTACGGGGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTACAACTTCA
TCCGAAGCATGGCCGCCTACAGCCTCCTGCTGTTCCTGCTGCAGATCAAG
GACAGACACAACGGCAACATTATGCTGGACAAGAAGGGTCATATCATCCA
CATCGACTTTGGCTTCATGTTTGAAAGCTCGCCGGGCGGCAATCTCGGCT
GGGAACCCGACATCAAGCTGACGGATGAGATGGTGATGATCATGGGGGGC
AAGATGGAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAGG
CTACCTGGCTGTGCGGCCCTACATGGACGCGGTCGTCTCCCTGGTCACTC
TCATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCAGACAATCAAGCTC
TTGAAGCACAGGTTTAGCCCCAACATGACTGAGCGCGAGGCTGCAAATTT
CATCATGAAGGTCATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGGACCT
ACGACATGATCCAGTACTATCAGAATGACATCCCCTACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMCPVDFHGIFQLDERRRDAV
IALGIFLIESDLQHKDCVVPYLLRLLKGLPKVYWVEESTARKGRGALPVA
ESFSFCLVTLLSDVAYRDPSLRDEILEVLLQVLHVLLGMCQALEIQDKEY
LCKYAIPCLIGISRAFGRYSNMEESLLSKLFPKIPPHSLRVLEELEGVRR
RSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGMPPPSSPGG
SAFHYFEASCLPDGTALEPEYYFSTISSSFSVSPLFNGVTYKEFNIPLEM
LRELLNLVKKIVEEAVLKSLDAIVASVMEANPSADLYYTSFSDPLYLTMF
KMLRDTLYYMKDLPTSFVKEIHDFVLEQFNTSQGELQKILHDADRIHNEL
SPLKLRCQASAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAH
LPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQY
HTVAGNDIKISVTNEHSESTLNVMSGKKSQPSMYEQLRDIAIDNICRCLK
AGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALR
DTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNL
FQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLVDE
LLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLT
RRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIAT
KSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEV
SALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKD
KSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEELERHAQFLLVNFNHI
HKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKD
QPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKS
HLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLSER
PACVKKDYSNFMASLNLRNRYAGEVYGMIRFSGTTGQMSDLNKMMVQDLH
SALDRSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGME
TALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKFGLFSAEIKEADPLA
ASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSM
SLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKI
YSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVP
PDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRG
SQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANW
RSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRL
DPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMY
PPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAS
KSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAK
DFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPS
NPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDS
EDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLD
LFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDES
TLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGF
MFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVR
PYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVI
QSCFLSNRSRTYDMIQYYQNDIPY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU14236
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS tag for baculovirus expression.; ; Estimated MW of expressed protein : 235000 Daltons