cDNA Clone PIK4CA

• Plasmid: pGEX6P-1-PIK4CA
• Parent Plasmid: pGEX6P1
• Expressed: GST-PIK4CA
• Genbank: NM_058004.2
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGTGCCCAGTAGATTTTCATGGGATCTTCCAGTTAGATGAAAG
ACGGAGAGATGCAGTGATTGCATTGGGCATTTTTCTGATTGAATCTGATC
TTCAGCACAAAGATTGTGTGGTTCCTTACCTTCTTCGACTTCTCAAAGGT
CTTCCAAAAGTGTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGG
TGCCCTCCCGGTTGCAGAGAGCTTCAGCTTCTGCTTGGTAACTCTGCTGT
CTGATGTGGCCTATAGGGATCCTTCACTTAGGGATGAGATTTTAGAGGTG
CTTTTGCAGGTTTTGCATGTCCTCTTGGGGATGTGCCAGGCCTTGGAGAT
TCAAGACAAAGAATACCTTTGCAAGTATGCTATCCCATGCCTGATAGGAA
TCTCGCGAGCATTTGGGCGTTACAGCAACATGGAAGAGTCTCTCCTCTCA
AAGCTCTTTCCCAAAATCCCTCCTCATTCCCTCCGTGTCCTGGAAGAGCT
TGAAGGTGTTCGAAGGCGTTCCTTTAATGACTTCCGCTCCATCCTCCCCA
GCAATCTGCTGACTGTCTGTCAGGAGGGTACCCTGAAGAGGAAAACCAGC
AGTGTGTCCAGCATCTCTCAGGTCAGCCCTGAACGCGGCATGCCCCCTCC
CAGTTCCCCTGGAGGATCTGCCTTTCACTACTTTGAAGCCTCCTGTTTGC
CCGATGGGACTGCCCTAGAGCCTGAGTACTACTTTTCAACCATCAGCTCC
AGCTTCTCAGTCTCTCCCCTTTTCAACGGTGTCACATATAAGGAGTTTAA
CATTCCATTGGAAATGCTTCGGGAACTCTTAAACCTGGTGAAGAAGATCG
TTGAGGAGGCTGTTCTCAAATCTTTGGATGCCATTGTAGCCAGTGTGATG
GAGGCCAACCCCAGTGCTGATCTCTACTACACTTCCTTCAGTGACCCTCT
CTACCTGACCATGTTCAAGATGCTGCGTGACACTCTGTACTACATGAAGG
ACCTCCCGACCTCTTTTGTGAAGGAGATCCATGATTTTGTGCTGGAGCAG
TTCAACACGAGCCAGGGGGAGCTCCAGAAGATTCTACATGACGCAGACCG
GATCCACAATGAGCTGAGCCCCCTCAAACTGCGCTGTCAGGCGAGTGCTG
CCTGTGTGGACCTCATGGTGTGGGCTGTGAAGGACGAGCAGGGTGCAGAA
AACCTTTGCATCAAGCTATCTGAGAAGCTGCAGTCCAAGACGTCCAGCAA
AGTCATTATTGCTCACTTGCCCCTGCTGATCTGCTGTCTGCAGGGTTTGG
GCCGCCTGTGCGAGAGGTTCCCGGTGGTGGTGCACTCTGTGACACCGTCC
TTGCGAGACTTCCTGGTCATCCCGTCCCCAGTTCTGGTGAAGCTCTACAA
GTACCACAGTCAGTACCACACAGTTGCTGGCAATGATATAAAAATCAGTG
TGACCAATGAGCATTCCGAGTCAACCCTGAACGTCATGTCGGGTAAGAAG
AGCCAGCCCTCCATGTACGAGCAGCTCCGAGACATCGCTATTGACAACAT
CTGCAGGTGCCTGAAGGCTGGATTGACGGTGGACCCAGTGATTGTGGAGG
CGTTCTTGGCCAGCCTGTCCAACCGGCTCTACATCTCTCAGGAGAGCGAC
AAGGACGCTCACTTGATTCCCGACCACACAATCCGAGCCTTGGGACACAT
TGCGGTGGCCTTGAGGGACACCCCGAAGGTCATGGAGCCCATTCTGCAGA
TCCTACAGCAGAAATTCTGCCAGCCACCCTCCCCCCTCGATGTGCTGATT
ATTGACCAGCTGGGCTGCCTGGTCATCACCGGAAATCAATACATCTATCA
GGAAGTGTGGAACCTCTTCCAGCAGATCAGTGTGAAGGCCAGCTCCGTTG
TATACTCAGCCACCAAAGATTACAAGGACCACGGCTATAGGCATTGCTCC
CTGGCAGTGATTAATGCCCTGGCCAACATCGCGGCCAACATCCAAGACGA
GCACCTGGTGGATGAGCTGCTCATGAACCTGTTGGAGTTGTTTGTGCAGC
TGGGGCTGGAGGGGAAGCGAGCCAGCGAGAGGGCAAGCGAGAAGGGCCCT
GCCCTAAAGGCTTCTAGCAGTGCAGGGAACTTGGGAGTACTCATTCCTGT
AATAGCTGTGCTCACCCGACGACTGCCACCCATCAAAGAAGCTAAGCCTC
GGTTACAGAAGCTCTTCCGAGACTTCTGGCTGTATTCCGTTCTGATGGGA
TTCGCTGTGGAGGGCTCAGGACTCTGGCCAGAAGAATGGTACGAGGGGGT
CTGTGAAATAGCCACTAAGTCCCCCTTGCTCACCTTTCCCAGCAAGGAGC
CACTGCGGTCCGTCCTCCAGTATAACTCAGCCATGAAGAATGACACGGTC
ACCCCCGCTGAGCTGAGTGAGCTCCGCAGCACTATCATCAACCTGCTGGA
CCCCCCTCCCGAGGTGTCCGCACTCATCAACAAGCTGGACTTCGCCATGT
CCACCTACCTCCTCTCTGTGTACCGGCTGGAGTACATGAGGGTACTGCGT
TCAACAGATCCTGATCGCTTCCAGGTAATGTTCTGCTACTTTGAGGATAA
AGCTATTCAGAAAGACAAATCTGGGATGATGCAGTGTGTGATTGCAGTCG
CGGACAAAGTATTCGATGCCTTCCTGAACATGATGGCGGATAAAGCCAAG
ACCAAGGAGAACGAGGAGGAGCTGGAGCGGCACGCTCAGTTCCTGTTGGT
GAACTTCAACCACATCCACAAGAGGATAAGGAGGGTGGCAGACAAGTATC
TATCTGGTCTGGTGGATAAGTTTCCCCACTTGCTCTGGAGCGGGACTGTG
CTGAAGACCATGCTGGACATCCTGCAGACCCTGTCACTGTCACTGAGCGC
TGATATTCACAAGGATCAGCCTTACTATGACATCCCCGACGCCCCCTACC
GGATCACGGTTCCTGACACGTACGAAGCCCGTGAGAGCATTGTGAAGGAC
TTCGCTGCACGCTGTGGGATGATCCTCCAGGAGGCCATGAAGTGGGCACC
TACCGTCACCAAGTCCCACCTGCAGGAATATCTGAACAAACATCAGAACT
GGGTATCGGGACTGTCCCAGCACACGGGGCTGGCCATGGCCACTGAGAGC
ATCCTTCACTTTGCTGGCTACAACAAGCAGAACACAACTCTTGGGGCAAC
TCAGCTGAGCGAGCGCCCGGCCTGTGTGAAGAAAGACTACTCCAACTTCA
TGGCATCCCTGAATCTGCGCAACCGCTACGCGGGCGAGGTGTATGGAATG
ATTCGGTTCTCAGGCACCACAGGCCAGATGTCTGACCTGAACAAAATGAT
GGTCCAGGATCTACATTCAGCTTTAGACCGCAGTCATCCTCAGCACTACA
CGCAGGCCATGTTCAAGCTGACCGCAATGCTCATTAGCAGTAAAGATTGT
GACCCGCAGCTCCTTCATCATCTGTGCTGGGGTCCCCTCCGGATGTTCAA
TGAGCATGGCATGGAGACGGCCCTGGCCTGCTGGGAGTGGCTGCTGGCTG
GCAAGGATGGAGTGGAAGTGCCGTTCATGCGGGAGATGGCAGGGGCCTGG
CACATGACGGTGGAGCAGAAATTTGGCCTGTTTTCTGCTGAGATAAAGGA
AGCAGACCCCCTGGCTGCCTCGGAAGCAAGTCAACCCAAACCCTGTCCCC
CCGAAGTGACCCCCCACTACATCTGGATCGACTTCCTGGTGCAGCGGTTT
GAGATCGCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCAGCCT
GCTGCAGCGCTCCATGTCCCTGAACATCGGCGGGGCCAAGGGGAGCATGA
ACCGGCACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGACCCTGGGG
CTGTCCCTCCTGCATGCCGATGTGGTTCCAAATGCAACCATCCGCAATGT
GCTTCGCGAGAAGATCTACTCCACTGCCTTTGACTACTTCAGCTGTCCCC
CAAAGTTCCCTACTCAAGGAGAGAAGCGGCTGCGTGAAGACATAAGCATC
ATGATTAAATTTTGGACCGCCATGTTCTCAGATAAGAAGTACCTGACCGC
CAGCCAGCTTGTTCCCCCAGATAATCAGGACACCCGGAGCAACCTGGACA
TAACTGTCGGCTCTCGGCAACAAGCCACCCAAGGCTGGATCAACACATAC
CCCCTGTCCAGCGGCATGTCCACCATCTCCAAGAAATCAGGCATGTCTAA
GAAAACCAACCGGGGCTCCCAGCTGCACAAATACTACATGAAGCGCAGGA
CGCTGCTGCTGTCCCTGCTGGCCACTGAGATCGAGCGTCTCATCACATGG
TACAACCCGCTGTCAGCCCCGGAACTGGAACTAGACCAGGCCGGAGAGAA
CAGCGTGGCCAACTGGAGATCTAAGTACATCAGCCTGAGTGAGAAGCAGT
GGAAGGACAACGTGAACCTCGCCTGGAGCATCTCTCCCTACCTAGCCGTG
CAGCTGCCTGCCAGGTTTAAGAACACAGAAGCCATTGGGAACGAAGTGAC
CCGTCTCGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCCTGAAGCAA
TCAAGTTCCTGGTCACCTGGCACACCATCGACGCCGATGCTCCAGAGCTC
AGCCATGTGCTGTGCTGGGCGCCCACGGACCCACCCACAGGCCTCTCCTA
CTTCTCCAGCATGTACCCGCCGCACCCTCTCACGGCGCAGTACGGGGTGA
AAGTCCTGCGGTCCTTCCCTCCGGACGCCATCCTCTTCTACATCCCCCAG
ATTGTGCAGGCCCTCAGGTACGACAAGATGGGCTATGTGCGGGAGTATAT
TCTGTGGGCAGCGTCTAAATCCCAGCTTCTGGCACACCAGTTCATCTGGA
ACATGAAGACTAACATTTATCTAGATGAAGAGGGCCACCAGAAAGACCCT
GACATCGGCGACCTCCTGGATCAGTTGGTAGAGGAGATCACAGGCTCCTT
GTCCGGCCCAGCGAAGGACTTTTACCAGCGGGAGTTTGATTTCTTTAACA
AGATCACCAACGTGTCGGCTATCATCAAGCCCTACCCTAAAGGCGACGAG
AGAAAGAAGGCTTGTCTGTCGGCCCTGTCTGAAGTGAAGGTGCAGCCGGG
CTGCTACCTGCCCAGCAACCCTGAGGCCATTGTGCTGGACATCGACTACA
AGTCTGGGACCCCGATGCAGAGTGCTGCAAAAGCCCCATATCTGGCCAAG
TTCAAGGTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAAGGTCTGCG
GTGCCGCTCAGACTCCGAGGATGAGTGCAGCACGCAGGAGGCCGACGGCC
AGAAGATCTCCTGGCAGGCAGCCATCTTCAAGGTGGGAGACGACTGCCGG
CAGGACATGCTGGCCCTGCAGATCATCGACCTCTTCAAGAACATCTTCCA
GCTGGTCGGCCTGGACCTCTTTGTTTTTCCCTACCGCGTGGTGGCCACTG
CCCCTGGGTGCGGGGTGATCGAGTGCATCCCCGACTGCACCTCCCGGGAC
CAGCTGGGCCGCCAGACAGACTTCGGCATGTACGACTACTTCACACGCCA
GTACGGGGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTACAACTTCA
TCCGAAGCATGGCCGCCTACAGCCTCCTGCTGTTCCTGCTGCAGATCAAG
GACAGACACAACGGCAACATTATGCTGGACAAGAAGGGTCATATCATCCA
CATCGACTTTGGCTTCATGTTTGAAAGCTCGCCGGGCGGCAATCTCGGCT
GGGAACCCGACATCAAGCTGACGGATGAGATGGTGATGATCATGGGGGGC
AAGATGGAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAGG
CTACCTGGCTGTGCGGCCCTACATGGACGCGGTCGTCTCCCTGGTCACTC
TCATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCAGACAATCAAGCTC
TTGAAGCACAGGTTTAGCCCCAACATGACTGAGCGCGAGGCTGCAAATTT
CATCATGAAGGTCATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGGACCT
ACGACATGATCCAGTACTATCAGAATGACATCCCCTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMCPVDFHGIFQLDERR
RDAVIALGIFLIESDLQHKDCVVPYLLRLLKGLPKVYWVEESTARKGRGA
LPVAESFSFCLVTLLSDVAYRDPSLRDEILEVLLQVLHVLLGMCQALEIQ
DKEYLCKYAIPCLIGISRAFGRYSNMEESLLSKLFPKIPPHSLRVLEELE
GVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGMPPPS
SPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSVSPLFNGVTYKEFNI
PLEMLRELLNLVKKIVEEAVLKSLDAIVASVMEANPSADLYYTSFSDPLY
LTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNTSQGELQKILHDADRI
HNELSPLKLRCQASAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKV
IIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKY
HSQYHTVAGNDIKISVTNEHSESTLNVMSGKKSQPSMYEQLRDIAIDNIC
RCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIA
VALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQE
VWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEH
LVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVI
AVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVC
EIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDP
PPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKA
IQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEELERHAQFLLVN
FNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSAD
IHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPT
VTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQ
LSERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSGTTGQMSDLNKMMV
QDLHSALDRSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNE
HGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKFGLFSAEIKEA
DPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLL
QRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVL
REKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTAS
QLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKK
TNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENS
VANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTR
LVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYF
SSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYIL
WAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLS
GPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGC
YLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRC
RSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQL
VGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQY
GDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHI
DFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGY
LAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFI
MKVIQSCFLSNRSRTYDMIQYYQNDIPY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU14235
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST tag for bacterial expression.; ; Estimated MW of expressed protein : 258322 Daltons