cDNA Clone CHKA

• Plasmid: pGEX6P-1-CHKA
• Parent Plasmid: pGEX6P1
• Expressed: GST-CHKA
• Genbank: NM_001277.2
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCC
GCTCGGGCTGCTGCTGAGCTGCGGTAGCGGCAGCGCGGCCCCGGCGCCCG
GCGTGGGGCAGCAGCGCGACGCCGCCAGCGACCTCGAGTCCAAGCAGCTG
GGCGGCCAACAGCCGCCGCTCGCGCTGCCCCCTCCGCCGCCGCTGCCGCT
GCCGCTGCCGCTGCCCCAGCCCCCGCCGCCGCAGCCGCCCGCAGACGAGC
AGCCGGAGCCCCGGACGCGGCGCAGGGCCTATCTGTGGTGCAAGGAGTTC
CTGCCCGGCGCCTGGCGGGGCCTCCGCGAGGACGAGTTCCACATCAGTGT
CATCAGAGGCGGCCTTAGCAACATGCTGTTCCAGTGCTCCCTACCTGACA
CCACAGCCACCCTTGGTGATGAGCCTCGGAAAGTGCTCCTGCGGCTGTAT
GGAGCGATTTTGCAGATGAGGTCCTGTAATAAAGAGGGATCCGAACAAGC
TCAGAAAGAAAATGAATTTCAAGGGGCTGAGGCCATGGTTCTGGAGAGCG
TTATGTTTGCCATTCTCGCAGAGAGGTCACTTGGGCCAAAACTCTATGGC
ATCTTTCCCCAAGGCCGACTGGAGCAGTTCATCCCGAGCCGGCGATTAGA
TACTGAAGAATTAAGTTTGCCAGATATTTCTGCAGAAATCGCCGAGAAAA
TGGCTACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAATGG
CTTTTTGGCACAATGGAAAAGTATCTAAAGGAAGTGCTGAGAATTAAATT
TACTGAGGAATCCAGAATTAAAAAGCTCCACAAATTGCTCAGTTACAATC
TGCCCTTGGAACTGGAAAACCTGAGATCATTGCTTGAATCTACTCCATCT
CCAGTTGTATTTTGTCATAATGACTGTCAAGAAGGTAATATCTTGTTGCT
GGAAGGCCGAGAGAATTCTGAAAAACAGAAACTGATGCTCATTGATTTCG
AATACAGCAGTTACAATTACAGGGGATTCGACATTGGAAATCACTTCTGT
GAGTGGATGTATGATTATAGCTATGAAAAATACCCTTTTTTCAGAGCAAA
CATCCGGAAGTATCCCACCAAGAAACAACAGCTCCATTTTATTTCCAGTT
ACTTGCCTGCATTCCAAAATGACTTTGAAAACCTCAGTACTGAAGAAAAA
TCCATTATAAAAGAAGAAATGTTGCTTGAAGTTAATAGGTTTGCCCTTGC
ATCTCATTTCCTCTGGGGACTGTGGTCCATTGTACAAGCCAAGATTTCAT
CTATTGAATTTGGGTACATGGACTACGCCCAAGCAAGGTTTGATGCCTAT
TTCCACCAGAAGAGGAAGCTTGGGGTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKTKFCTGGEAEPSPLGLL
LSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPPPPLPLPLPL
PQPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGG
LSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEGSEQAQKEN
EFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEEL
SLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEES
RIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRE
NSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKY
PTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFL
WGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU14189
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 79071 Daltons