cDNA Clone UBE2J2

• Plasmid: pET28a(+) UBE2J2
• Parent Plasmid: pET28a original
• Expressed: 6H-UBE2J2
• Genbank: NM_058167
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCA
GAGGCTGAAGCAGGACTACCTTCGCATTAAGAAAGACCCGGTGCCTTACA
TCTGTGCCGAGCCCCTCCCTTCGAATATTCTCGAGTGGCACTATGTCGTC
CGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTATTATCATGGAAAACT
AATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCAGTATCTATATGATCA
CTCCCAACGGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTATCACG
GATTTCCACCCGGACACGTGGAACCCGGCCTGGTCTGTCTCCACCATCCT
GACTGGGCTCCTGAGCTTCATGGTGGAGAAGGGCCCCACCCTGGGCAGTA
TAGAGACGTCGGACTTCACGAAAAGACAACTGGCAGTGCAGAGTTTAGCA
TTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTCGTGGA
GGAGATTAAACAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGACCCC
AGACTCTCCCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTCGTC
CAGAACGGGATTCAGCTGCTCAACGGGCATGCGCCGGGGGCCGTCCCAAA
CCTCGCAGGGCTCCAGCAGGCCAACCGGCACCACGGACTCCTGGGTGGCG
CCCTGGCGAACTTGTTTGTGATAGTTGGGTTTGCAGCCTTTGCTTACACG
GTCAAGTACGTGCTGAGGAGCATCGCGCAGGAGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMSSTSSKRAPTTATQR
LKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLI
FPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILT
GLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEE
IKQKQKAQDELSSRPQTLPLPDVVPDGETHLVQNGIQLLNGHAPGAVPNL
AGLQQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU14061
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 28897 Daltons