cDNA Clone UBE2F

• Plasmid: pET28a(+) UBE2F
• Parent Plasmid: pET28a original
• Expressed: 6H-UBE2F
• Genbank: NM_080678
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGTCTCAA
AGGGTCCCGGACGGCAGCCACAGCGTCCGACTCGACTCGGAGGGTTTCTG
TGAGAGACAAATTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAATTTA
CCTTGTACATGTAAAGTGCATTTTCCTGATCCAAACAAGCTTCATTGTTT
TCAGCTAACAGTAACCCCAGATGAGGGTTACTACCAGGGTGGAAAATTTC
AGTTTGAAACTGAAGTTCCCGATGCGTACAACATGGTGCCTCCCAAAGTG
AAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAAT
ATGTCTGAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTC
CCACAAGAACATTAAAGGATGTCGTTTGGGGATTAAACTCTTTGTTTACT
GATCTTTTGAATTTTGATGATCCACTGAATATTGAAGCTGCAGAACATCA
TTTGCGGGACAAGGAGGACTTCCGGAATAAAGTGGATGACTACATCAAAC
GTTATGCCAGATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMLTLASKLKRDDGLKG
SRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQ
LTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEIC
LSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHL
RDKEDFRNKVDDYIKRYAR*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU14052
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 24620 Daltons