cDNA Clone PIK4CB

• Plasmid: pFBHTb PIK4CB delta1-12 delta316-330
• Parent Plasmid: pFastBac HTb
• Expressed: 6H-PIK4CB
• Genbank: AAH40300
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGATCTATGGGAGATACAGTAG
TGGAGCCTGCCCCCTTGAAGCCAACTTCTGAGCCCACTTCTGGCCCACCA
GGGAATAATGGGGGGTCCCTGCTAAGTGTCATCACGGAGGGGGTCGGGGA
ACTATCAGTGATTGACCCTGAGGTGGCCCAGAAGGCCTGCCAGGAGGTGT
TGGAGAAAGTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGC
ACCCCACTGGAGTTGGTCAATGGGGATGGTGTGGACAGTGAGATCCGTTG
CCTAGATGATCCACCTGCCCAGATCAGGGAGGAGGAAGATGAGATGGGGG
CCGCTGTGGCCTCAGGCACAGCCAAAGGAGCAAGAAGACGGCGGCAGAAC
AACTCAGCTAAACAGTCTTGGCTGCTGAGGCTGTTTGAGTCAAAACTGTT
TGACATCTCCATGGCCATTTCATACCTGTATAACTCCAAGGAGCCTGGAG
TACAAGCCTACATTGGCAACCGGCTCTTCTGCTTTCGCAACGAGGACGTG
GACTTCTATCTGCCCCAGTTGCTTAACATGTACATCCACATGGATGAGGA
CGTGGGTGATGCCATTAAGCCCTACATAGTCCACCGTTGCCGCCAGAGCA
TTAACTTTTCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTATTCTTCAGAC
ATGCACATTTCCACTCAACGACACTCCCGTGGGACCAAGCTACGGAAGCT
GATCCTCTCAGATGAGCTAAAGCCAGCTCACAGGAAGAGGGAGCTGCCCT
CCTTGAGCCCGGCCCCTGATACAGGGCTGTCTCCCTCCAAAAGGACTCAC
CAGCGCTCTAAGTCAGATGCCACTGCCAGCATAAGTCTCAGCAGCAACCT
GAAACGAACAGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGCCTGTTC
GACTGGCTCCTGAGAGAGAATTCATCAAGTCCCTGATGGCGATCGGCAAG
CGGCTGGCCACGCTCCCCACCAAAGAGCAGAAAACACAGAGGCTGATCTC
AGAGCTCTCCCTGCTCAACCATAAGCTCCCTGCCCGAGTCTGGCTGCCCA
CTGCTGGCTTTGACCACCACGTGGTCCGTGTACCCCACACACAGGCTGTT
GTCCTCAACTCCAAGGACAAGGCTCCCTACCTGATTTATGTGGAAGTCCT
TGAATGTGAAAACTTTGACACCACCAGTGTCCCTGCCCGGATCCCCGAGA
ACCGAATTCGGAGTACGAGGTCCGTAGAAAACTTGCCCGAATGTGGTATT
ACCCATGAGCAGCGAGCTGGCAGCTTCAGCACTGTGCCCAACTATGACAA
CGATGATGAGGCCTGGTCGGTGGATGACATAGGCGAGCTGCAAGTGGAGC
TCCCCGAAGTGCATACCAACAGCTGTGACAACATCTCCCAGTTCTCTGTG
GACAGCATCACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTGCAGCAGG
GGACATCCGCCGGCGCCTTTCGGAACAGCTGGCTCATACCCCGACAGCCT
TCAAACGAGACCCAGAAGATCCTTCTGCAGTTGCTCTCAAAGAGCCCTGG
CAGGAGAAAGTACGGCGGATCAGAGAGGGCTCCCCCTACGGCCATCTCCC
CAATTGGCGGCTCCTGTCAGTCATTGTCAAGTGTGGGGATGACCTTCGGC
AAGAGCTTCTGGCCTTTCAGGTGTTGAAGCAACTGCAGTCCATTTGGGAA
CAGGAGCGAGTGCCCCTTTGGATCAAGCCATACAAGATTCTTGTGATTTC
GGCTGATAGTGGCATGATTGAACCAGTGGTCAATGCTGTGTCCATCCATC
AGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAGGAG
CACGGCAGTTACACCACTGAGGCATTCCTCAGTGCACAGCGCAATTTTGT
GCAAAGTTGTGCTGGGTACTGCTTGGTCTGCTACCTGCTGCAAGTCAAGG
ACAGACACAATGGGAATATCCTTTTGGACGCAGAAGGCCACATCATCCAC
ATCGACTTTGGCTTCATCCTCTCCAGCTCACCCCGAAATCTGGGCTTTGA
GACGTCAGCCTTTAAGCTGACCACAGAGTTTGTGGATGTGATGGGCGGCC
TGGATGGCGACATGTTCAACTACTATAAGATGCTGATGCTGCAAGGGCTG
ATTGCCGCTCGGAAACACATGGACAAGGTGGTGCAGATCGTGGAGATCAT
GCAGCAAGGTTCTCAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGAA
ACCTCAAAGAGAGGTTCCACATGAGCATGACTGAGGAGCAGCTGCAGCTG
CTGGTGGAGCAGATGGTGGATGGCAGTATGCGGTCTATCACCACCAAACT
CTATGACGGCTTCCAGTACCTCACCAACGGCATCATGTGAGCGGCCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMGDTVVEPAPLKPTSEPTSGPP
GNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAVSSRG
TPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQN
NSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDV
DFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSSD
MHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPSKRTH
QRSKSDATASISLSSNLKRTASNPKVENEDEPVRLAPEREFIKSLMAIGK
RLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAV
VLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGI
THEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSV
DSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPW
QEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWE
QERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQE
HGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIH
IDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGL
IAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQL
LVEQMVDGSMRSITTKLYDGFQYLTNGIM*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU14025
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H. Compare with protein sequence with NP_002642.; ; Estimated MW of expressed protein : 93176 Daltons