cDNA Clone GYS2

• Plasmid: pGEX6P-1-GYS2
• Parent Plasmid: pGEX6P1
• Expressed: GST-GYS2
• Genbank: BC126310
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGCTTC
GAGGCCGATCCCTCTCTGTAACATCCCTGGGTGGGCTTCCCCAGTGGGAA
GTCGAAGAACTTCCTGTGGAGGAGTTACTGCTCTTTGAAGTTGCTTGGGA
AGTGACCAATAAAGTTGGAGGCATCTATACTGTGATTCAGACAAAGGCCA
AAACAACAGCAGATGAATGGGGAGAGAACTATTTTCTGATAGGTCCATAT
TTTGAGCATAATATGAAGACTCAGGTGGAACAGTGTGAACCTGTAAATGA
TGCTGTCAGAAGAGCAGTGGACGCAATGAATAAGCATGGCTGCCAGGTGC
ATTTTGGAAGATGGCTGATAGAAGGAAGTCCTTATGTGGTACTTTTTGAC
ATAGGCTATTCAGCTTGGAATCTGGACAGGTGGAAGGGTGACCTCTGGGA
AGCATGCAGTGTCGGCATTCCTTATCATGACCGAGAAGCCAATGATATGC
TGATATTTGGATCTTTAACTGCCTGGTTCTTAAAAGAGGTGACAGATCAT
GCAGATGGTAAATATGTCGTTGCCCAATTCCATGAATGGCAGGCTGGAAT
TGGACTGATCCTTTCTCGAGCCAGGAAACTTCCTATTGCCACAATATTTA
CAACCCACGCTACACTACTTGGGAGGTATCTCTGTGCAGCAAATATTGAT
TTCTACAACCATCTTGATAAGTTTAACATTGACAAAGAGGCTGGGGAAAG
GCAGATTTACCACCGGTACTGCATGGAGCGAGCTTCCGTTCATTGCGCTC
ACGTGTTCACCACGGTTTCTGAAATAACAGCAATAGAAGCTGAACATATG
CTGAAGAGAAAGCCTGATGTAGTTACTCCAAACGGCTTGAATGTTAAGAA
ATTTTCAGCAGTGCATGAGTTTCAAAATCTACATGCCATGTACAAGGCCA
GAATCCAAGATTTTGTTCGAGGTCATTTCTATGGTCATCTCGACTTTGAT
CTTGAAAAGACTTTGTTCCTTTTCATTGCTGGGAGGTATGAGTTTTCAAA
CAAAGGAGCTGACATCTTCCTAGAATCCTTATCCAGGCTAAATTTCCTGC
TGAGGATGCATAAAAGTGACATCACAGTGGTGGTGTTTTTCATTATGCCT
GCCAAGACAAATAATTTCAACGTGGAAACCCTGAAAGGACAAGCAGTGCG
AAAACAGCTGTGGGATGTTGCACATTCTGTGAAGGAAAAGTTTGGAAAAA
AACTCTATGATGCATTATTAAGAGGAGAAATTCCTGACCTGAACGATATT
TTAGATCGAGATGATCTAACAATTATGAAAAGAGCCATCTTTTCAACTCA
GCGACAGTCATTGCCCCCAGTGACCACGCACAACATGATTGATGACTCCA
CCGACCCCATCCTCAGCACCATTAGACGGATTGGACTTTTCAACAACCGC
ACAGATAGAGTCAAGGTGATTTTGCACCCAGAGTTTCTATCCTCCACCAG
TCCCTTACTACCCATGGACTATGAAGAGTTTGTTAGAGGTTGTCATCTTG
GAGTATTTCCATCATACTATGAACCCTGGGGTTATACTCCAGCTGAATGC
ACTGTGATGGGTATCCCCAGTGTGACCACGAATCTCTCCGGGTTTGGCTG
TTTCATGCAGGAGCACGTGGCTGATCCTACTGCTTACGGTATTTACATCG
TTGACAGGCGGTTCCGTTCTCCAGATGATTCTTGCAATCAGCTGACTAAG
TTTCTCTATGGATTTTGCAAACAGTCACGCCGCCAAAGGATTATCCAGAG
GAACAGAACTGAGAGGCTCTCAGATCTTCTGGATTGGAGATACTTAGGCA
GATATTACCAGCATGCCAGACACCTGACATTAAGCAGAGCTTTTCCAGAT
AAATTCCATGTGGAACTAACATCACCACCAACGACAGAAGGATTTAAATA
TCCCAGGCCTTCCTCAGTACCACCTTCTCCTTCAGGGTCTCAGGCCTCCA
GTCCTCAGAGCAGTGATGTGGAAGATGAAGTGGAGGATGAGAGATACGAT
GAGGAAGAGGAGGCTGAAAGGGATCGGTTAAATATCAAGTCACCATTTTC
ACTGAGCCACGTTCCTCATGGGAAGAAAAAGCTGCATGGTGAATATAAGA
ACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLRGRSLSVTSLGGLPQWE
VEELPVEELLLFEVAWEVTNKVGGIYTVIQTKAKTTADEWGENYFLIGPY
FEHNMKTQVEQCEPVNDAVRRAVDAMNKHGCQVHFGRWLIEGSPYVVLFD
IGYSAWNLDRWKGDLWEACSVGIPYHDREANDMLIFGSLTAWFLKEVTDH
ADGKYVVAQFHEWQAGIGLILSRARKLPIATIFTTHATLLGRYLCAANID
FYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHM
LKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQDFVRGHFYGHLDFD
LEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDITVVVFFIMP
AKTNNFNVETLKGQAVRKQLWDVAHSVKEKFGKKLYDALLRGEIPDLNDI
LDRDDLTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNNR
TDRVKVILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPAEC
TVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFRSPDDSCNQLTK
FLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPD
KFHVELTSPPTTEGFKYPRPSSVPPSPSGSQASSPQSSDVEDEVEDERYD
EEEEAERDRLNIKSPFSLSHVPHGKKKLHGEYKN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4879
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.