cDNA Clone EPHB2

• Plasmid: pGEX6P-1-EPHB2 560-987
• Parent Plasmid: pGEX6P1
• Expressed: GST-EPHB2 560-987
• Genbank: NM_004442
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGTCATCG
CCATCGTGTGTAACAGAAGACGGGGGTTTGAGCGTGCTGACTCGGAGTAC
ACGGACAAGCTGCAACACTACACCAGTGGCCACATGACCCCAGGCATGAA
GATCTACATCGATCCTTTCACCTACGAGGACCCCAACGAGGCAGTGCGGG
AGTTTGCCAAGGAAATTGACATCTCCTGTGTCAAAATTGAGCAGGTGATC
GGAGCAGGGGAGTTTGGCGAGGTCTGCAGTGGCCACCTGAAGCTGCCAGG
CAAGAGAGAGATCTTTGTGGCCATCAAGACGCTCAAGTCGGGCTACACGG
AGAAGCAGCGCCGGGACTTCCTGAGCGAAGCCTCCATCATGGGCCAGTTC
GACCATCCCAACGTCATCCACCTGGAGGGTGTCGTGACCAAGAGCACACC
TGTGATGATCATCACCGAGTTCATGGAGAATGGCTCCCTGGACTCCTTTC
TCCGGCAAAACGATGGGCAGTTCACAGTCATCCAGCTGGTGGGCATGCTT
CGGGGCATCGCAGCTGGCATGAAGTACCTGGCAGACATGAACTATGTTCA
CCGTGACCTGGCTGCCCGCAACATCCTCGTCAACAGCAACCTGGTCTGCA
AGGTGTCGGACTTTGGGCTCTCACGCTTTCTAGAGGACGATACCTCAGAC
CCCACCTACACCAGTGCCCTGGGCGGAAAGATCCCCATCCGCTGGACAGC
CCCGGAAGCCATCCAGTACCGGAAGTTCACCTCGGCCAGTGATGTGTGGA
GCTACGGCATTGTCATGTGGGAGGTGATGTCCTATGGGGAGCGGCCCTAC
TGGGACATGACCAACCAGGATGTAATCAATGCCATTGAGCAGGACTATCG
GCTGCCACCGCCCATGGACTGCCCGAGCGCCCTGCACCAACTCATGCTGG
ACTGTTGGCAGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAAATTGTC
AACACGCTAGACAAGATGATCCGCAATCCCAACAGCCTCAAAGCCATGGC
GCCCCTCTCCTCTGGCATCAACCTGCCGCTGCTGGACCGCACGATCCCCG
ACTACACCAGCTTTAACACGGTGGACGAGTGGCTGGAGGCCATCAAGATG
GGGCAGTACAAGGAGAGCTTCGCCAATGCCGGCTTCACCTCCTTTGACGT
CGTGTCTCAGATGATGATGGAGGACATCCTCCGGGTTGGGGTCACTTTGG
CTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCGCAG
ATGAACCAGATTCAGTCTGTGGAGGTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVIAIVCNRRRGFERADSEY
TDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVI
GAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQF
DHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGML
RGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSD
PTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPY
WDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIV
NTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIPDYTSFNTVDEWLEAIKM
GQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQ
MNQIQSVEV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4877
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.