cDNA Clone EPHB4

• Plasmid: pGEX6P-1-EPHB4 561-987
• Parent Plasmid: pGEX6P1
• Expressed: GST-EPHB4 561-987
• Genbank: AY056047
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGGCCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCTCTGCC
TCAGGAAGCAGAGCAATGGGAGAGAAGCAGAATATTCGGACAAACACGGA
CAGTATCTCATCGGGCATGGTACTAAGGTCTACATCGACCCCTTCACTTA
TGAAGACCCTAATGAGGCTGTGAGGGAATTTGCAAAAGAGATCGATGTCT
CCTACGTCAAGATTGAAGAGGTGATTGGTGCAGGTGAGTTTGGCGAGGTG
TGCCGGGGGCGGCTCAAGGCCCCAGGGAAGAAGGAGAGCTGTGTGGCAAT
CAAGACCCTGAAGGGTGGCTACACGGAGCGGCAGCGGCGTGAGTTTCTGA
GCGAGGCCTCCATCATGGGCCAGTTCGAGCACCCCAATATCATCCGCCTG
GAGGGCGTGGTCACCAACAGCATGCCCGTCATGATTCTCACAGAGTTCAT
GGAGAACGGCGCCCTGGACTCCTTCCTGCGGCTAAACGACGGACAGTTCA
CAGTCATCCAGCTCGTGGGCATGCTGCGGGGCATCGCCTCGGGCATGCGG
TACCTTGCCGAGATGAGCTACGTCCACCGAGACCTGGCTGCTCGCAACAT
CCTAGTCAACAGCAACCTCGTCTGCAAAGTGTCTGACTTTGGCCTTTCCC
GATTCCTGGAGGAGAACTCTTCCGATCCCACCTACACGAGCTCCCTGGGA
GGAAAGATTCCCATCCGATGGACTGCCCCGGAGGCCATTGCCTTCCGGAA
GTTCACTTCCGCCAGTGATGCCTGGAGTTACGGGATTGTGATGTGGGAGG
TGATGTCATTTGGGGAGAGGCCGTACTGGGACATGAGCAATCAGGACGTG
ATCAATGCCATTGAACAGGACTACCGGCTGCCCCCGCCCCCAGACTGTCC
CACCTCCCTCCACCAGCTCATGCTGGACTGTTGGCAGAAAGACCGGAATG
CCCGGCCCCGCTTCCCCCAGGTGGTCAGCGCCCTGGACAAGATGATCCGG
AACCCCGCCAGCCTCAAAATCGTGGCCCGGGAGAATGGCGGGGCCTCACA
CCCTCTCCTGGACCAGCGGCAGCCTCACTACTCAGCTTTTGGCTCTGTGG
GCGAGTGGCTTCGGGCCATCAAAATGGGAAGATACGAAGAAAGTTTCGCA
GCCGCTGGCTTTGGCTCCTTCGAGCTGGTCAGCCAGATCTCTGCTGAGGA
CCTGCTCCGAATCGGAGTCACTCTGGCGGGACACCAGAAGAAAATCTTGG
CCAGTGTCCAGCACATGAAGTCCCAGGCCAAGCCGGGAACCCCGGGTGGG
ACAGGAGGACCGGCCCCGCAGTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGGHPPKSDLEVLFQGPLGSLCLRKQSNGREAEYSDKHG
QYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEV
CRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRL
EGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMR
YLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLG
GKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDV
INAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIR
NPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFA
AAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGG
TGGPAPQY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4863
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.