cDNA Clone EIF2C3

• Plasmid: pEGFP-C2-TAP EIF2C3
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP-EIF2C3
• Genbank: NM_024852
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGCCGGACTCAGATCTCGAGCTCAAGCTTC
GATATCGCCCTTGCCACCATGGACACAAGTGCCCACGATGAAGCCGTAGA
CAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATT
TACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGTTTAAAA
GATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAA
TGATGCTCAGGCGCCGAAAGTAGACAACAAATTCAACAAAGAACGACAAA
ACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGA
AACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCT
TTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGTAGACG
CGAGTTGGTCAGCGGGTGCTAGCGAGAACTTGTACTTCCAGGGCGGAAGA
TCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCGTCTCAGCAGC
CAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTC
CAACTGCTCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGGAAATC
GGCTCCGCAGGACCCGCTGGGGCCCAGCCCCTACTCATGGTGCCCAGAAG
ACCTGGCTATGGCACCATGGGCAAACCCATTAAACTGCTGGCTAACTGTT
TTCAAGTTGAAATCCCAAAGATTGATGTCTACCTCTATGAGGTAGATATT
AAACCAGACAAGTGTCCTAGGAGAGTGAACAGGGAGGTGGTTGACTCAAT
GGTTCAGCATTTTAAAGTAACTATATTTGGAGACCGTAGACCAGTTTATG
ATGGAAAAAGAAGTCTTTACACCGCCAATCCACTTCCTGTGGCAACTACA
GGGGTAGATTTAGACGTTACTTTACCTGGGGAAGGTGGAAAAGATCGACC
TTTCAAGGTGTCAATCAAATTTGTCTCTCGGGTGAGTTGGCACCTACTGC
ATGAAGTACTGACAGGACGGACCTTGCCTGAGCCACTGGAATTAGACAAG
CCAATCAGCACTAACCCTGTCCATGCCGTTGATGTGGTGCTACGACATCT
GCCCTCCATGAAATACACACCTGTGGGGCGTTCATTTTTCTCCGCTCCAG
AAGGATATGACCACCCTCTGGGAGGGGGCAGGGAAGTGTGGTTTGGATTC
CATCAGTCTGTTCGGCCTGCCATGTGGAAAATGATGCTTAATATCGATGT
TTCTGCCACTGCCTTCTACAAAGCACAACCTGTAATTCAGTTCATGTGTG
AAGTTCTTGATATTCATAATATTGATGAGCAGCCAAGACCTCTGACTGAT
TCTCATCGGGTAAAATTCACCAAAGAGATAAAAGGTTTGAAGGTTGAAGT
GACTCATTGTGGAACAATGAGACGGAAATACCGTGTTTGTAATGTAACAA
GGAGGCCTGCCAGTCATCAAACCTTTCCTTTACAGTTAGAAAACGGCCAA
ACTGTGGAGAGAACAGTAGCGCAGTATTTCAGAGAAAAGTATACTCTTCA
GCTGAAGTACCCGCACCTTCCCTGTCTGCAAGTCGGGCAGGAACAGAAAC
ACACCTACCTGCCACTAGAAGTCTGTAATATTGTGGCAGGGCAACGGTGT
ATCAAGAAGCTAACAGACAATCAGACTTCCACTATGATCAAGGCAACAGC
AAGATCTGCACCAGATAGACAAGAGGAAATTAGCAGATTGGTAAGAAGTG
CAAATTATGAAACAGATCCATTTGTTCAGGAGTTTCAATTTAAAGTTCGG
GATGAAATGGCTCATGTAACTGGACGCGTACTTCCAGCACCTATGCTCCA
GTATGGAGGACGGAATCGGACAGTAGCAACACCGAGCCATGGAGTATGGG
ACATGCGAGGGAAACAATTCCACACAGGAGTTGAAATCAAAATGTGGGCT
ATCGCTTGTTTTGCCACACAGAGGCAGTGCAGAGAAGAAATATTGAAGGG
TTTCACAGACCAGCTGCGTAAGATTTCTAAGGATGCAGGGATGCCCATCC
AGGGCCAGCCATGCTTCTGCAAATATGCACAGGGGGCAGACAGCGTAGAG
CCCATGTTCCGGCATCTCAAGAACACATATTCTGGCCTACAGCTTATTAT
CGTCATCCTGCCGGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGTAG
GAGACACACTTTTGGGTATGGCTACACAATGTGTTCAAGTCAAGAATGTA
ATAAAAACATCTCCTCAAACTCTGTCAAACTTGTGCCTAAAGATAAATGT
TAAACTCGGAGGGATCAATAATATTCTTGTACCTCATCAAAGACCTTCTG
TGTTCCAGCAACCAGTGATCTTTTTGGGAGCCGATGTCACTCATCCACCT
GCTGGTGATGGAAAGAAGCCTTCTATTGCTGCTGTTGTAGGTAGTATGGA
TGCACACCCAAGCAGATACTGTGCCACAGTAAGAGTTCAGAGACCCCGAC
AGGAGATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTTCTTATTCAA
TTTTATAAGTCAACTCGGTTCAAGCCTACTCGTATCATCTTTTATCGGGA
TGGTGTTTCAGAGGGGCAGTTTAGGCAGGTATTATATTATGAACTACTAG
CAATTCGAGAAGCCTGCATCAGTTTGGAGAAAGACTATCAACCTGGAATA
ACCTACATTGTAGTTCAGAAGAGACATCACACTCGATTATTTTGTGCTGA
TAGGACAGAAAGGGTTGGAAGAAGTGGCAATATCCCAGCTGGAACAACAG
TTGATACAGACATTACACACCCATATGAGTTCGATTTTTACCTCTGTAGC
CATGCTGGAATACAGGGTACCAGTCGTCCTTCACACTATCATGTTTTATG
GGATGATAACTGCTTTACTGCAGATGAACTTCAGCTGCTAACTTACCAGC
TCTGCCACACTTACGTACGCTGTACACGATCTGTTTCTATACCTGCACCA
GCGTATTATGCTCACCTGGTAGCATTTAGAGCCAGATATCATCTTGTGGA
CAAAGAACATGACAGTGCTGAAGGAAGTCACGTTTCAGGACAAAGCAATG
GGCGAGATCCACAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACC
TTACGCACAATGTACTTCGCTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMEI
GSAGPAGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDI
KPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATT
GVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDK
PISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGF
HQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTD
SHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQ
TVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRC
IKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVR
DEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWA
IACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVE
PMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNV
IKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPP
AGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQ
FYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGI
TYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCS
HAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAP
AYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDT
LRTMYFA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4847
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GFP-TAP.