cDNA Clone EIF2C3

• Plasmid: pGEX6P-1-EIF2C3
• Parent Plasmid: pGEX6P1
• Expressed: GST-EIF2C3
• Genbank: NM_024852
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGGAAA
TCGGCTCCGCAGGACCCGCTGGGGCCCAGCCCCTACTCATGGTGCCCAGA
AGACCTGGCTATGGCACCATGGGCAAACCCATTAAACTGCTGGCTAACTG
TTTTCAAGTTGAAATCCCAAAGATTGATGTCTACCTCTATGAGGTAGATA
TTAAACCAGACAAGTGTCCTAGGAGAGTGAACAGGGAGGTGGTTGACTCA
ATGGTTCAGCATTTTAAAGTAACTATATTTGGAGACCGTAGACCAGTTTA
TGATGGAAAAAGAAGTCTTTACACCGCCAATCCACTTCCTGTGGCAACTA
CAGGGGTAGATTTAGACGTTACTTTACCTGGGGAAGGTGGAAAAGATCGA
CCTTTCAAGGTGTCAATCAAATTTGTCTCTCGGGTGAGTTGGCACCTACT
GCATGAAGTACTGACAGGACGGACCTTGCCTGAGCCACTGGAATTAGACA
AGCCAATCAGCACTAACCCTGTCCATGCCGTTGATGTGGTGCTACGACAT
CTGCCCTCCATGAAATACACACCTGTGGGGCGTTCATTTTTCTCCGCTCC
AGAAGGATATGACCACCCTCTGGGAGGGGGCAGGGAAGTGTGGTTTGGAT
TCCATCAGTCTGTTCGGCCTGCCATGTGGAAAATGATGCTTAATATCGAT
GTTTCTGCCACTGCCTTCTACAAAGCACAACCTGTAATTCAGTTCATGTG
TGAAGTTCTTGATATTCATAATATTGATGAGCAGCCAAGACCTCTGACTG
ATTCTCATCGGGTAAAATTCACCAAAGAGATAAAAGGTTTGAAGGTTGAA
GTGACTCATTGTGGAACAATGAGACGGAAATACCGTGTTTGTAATGTAAC
AAGGAGGCCTGCCAGTCATCAAACCTTTCCTTTACAGTTAGAAAACGGCC
AAACTGTGGAGAGAACAGTAGCGCAGTATTTCAGAGAAAAGTATACTCTT
CAGCTGAAGTACCCGCACCTTCCCTGTCTGCAAGTCGGGCAGGAACAGAA
ACACACCTACCTGCCACTAGAAGTCTGTAATATTGTGGCAGGGCAACGGT
GTATCAAGAAGCTAACAGACAATCAGACTTCCACTATGATCAAGGCAACA
GCAAGATCTGCACCAGATAGACAAGAGGAAATTAGCAGATTGGTAAGAAG
TGCAAATTATGAAACAGATCCATTTGTTCAGGAGTTTCAATTTAAAGTTC
GGGATGAAATGGCTCATGTAACTGGACGCGTACTTCCAGCACCTATGCTC
CAGTATGGAGGACGGAATCGGACAGTAGCAACACCGAGCCATGGAGTATG
GGACATGCGAGGGAAACAATTCCACACAGGAGTTGAAATCAAAATGTGGG
CTATCGCTTGTTTTGCCACACAGAGGCAGTGCAGAGAAGAAATATTGAAG
GGTTTCACAGACCAGCTGCGTAAGATTTCTAAGGATGCAGGGATGCCCAT
CCAGGGCCAGCCATGCTTCTGCAAATATGCACAGGGGGCAGACAGCGTAG
AGCCCATGTTCCGGCATCTCAAGAACACATATTCTGGCCTACAGCTTATT
ATCGTCATCCTGCCGGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGT
AGGAGACACACTTTTGGGTATGGCTACACAATGTGTTCAAGTCAAGAATG
TAATAAAAACATCTCCTCAAACTCTGTCAAACTTGTGCCTAAAGATAAAT
GTTAAACTCGGAGGGATCAATAATATTCTTGTACCTCATCAAAGACCTTC
TGTGTTCCAGCAACCAGTGATCTTTTTGGGAGCCGATGTCACTCATCCAC
CTGCTGGTGATGGAAAGAAGCCTTCTATTGCTGCTGTTGTAGGTAGTATG
GATGCACACCCAAGCAGATACTGTGCCACAGTAAGAGTTCAGAGACCCCG
ACAGGAGATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTTCTTATTC
AATTTTATAAGTCAACTCGGTTCAAGCCTACTCGTATCATCTTTTATCGG
GATGGTGTTTCAGAGGGGCAGTTTAGGCAGGTATTATATTATGAACTACT
AGCAATTCGAGAAGCCTGCATCAGTTTGGAGAAAGACTATCAACCTGGAA
TAACCTACATTGTAGTTCAGAAGAGACATCACACTCGATTATTTTGTGCT
GATAGGACAGAAAGGGTTGGAAGAAGTGGCAATATCCCAGCTGGAACAAC
AGTTGATACAGACATTACACACCCATATGAGTTCGATTTTTACCTCTGTA
GCCATGCTGGAATACAGGGTACCAGTCGTCCTTCACACTATCATGTTTTA
TGGGATGATAACTGCTTTACTGCAGATGAACTTCAGCTGCTAACTTACCA
GCTCTGCCACACTTACGTACGCTGTACACGATCTGTTTCTATACCTGCAC
CAGCGTATTATGCTCACCTGGTAGCATTTAGAGCCAGATATCATCTTGTG
GACAAAGAACATGACAGTGCTGAAGGAAGTCACGTTTCAGGACAAAGCAA
TGGGCGAGATCCACAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATA
CCTTACGCACAATGTACTTCGCTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEIGSAGPAGAQPLLMVPR
RPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDS
MVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDR
PFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRH
LPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNID
VSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVE
VTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTL
QLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKAT
ARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPML
QYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILK
GFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLI
IVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKIN
VKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSM
DAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYR
DGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCA
DRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVL
WDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLV
DKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4846
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.