cDNA Clone PAK2

• Plasmid: pFBHTb PAK2 G258R T402E
• Parent Plasmid: pFastBac HTb
• Expressed: His-PAK2 G258R T402E
• Genbank: BC069613
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGATCCATGTCTGATAACGGAG
AACTGGAAGATAAGCCTCCAGCACCTCCTGTGCGAATGAGCAGCACCATC
TTTAGCACTGGAGGCAAAGACCCTTTGTCAGCCAATCACAGTTTGAAACC
TTTGCCCTCTGTTCCAGAAGAGAAAAAGCCCAGGCATAAAATCATCTCCA
TATTCTCAGGCACAGAGAAAGGAAGTAAAAAGAAGGAAAAGGAACGGCCA
GAAATTTCTCCTCCATCTGATTTTGAGCACACCATCCATGTTGGTTTTGA
TGCTGTTACTGGAGAATTCACTGGCATGCCAGAACAGTGGGCTCGATTAC
TACAGACCTCCAATATCACCAAACTAGAGCAAAAGAAGAACCCTCAGGCT
GTGCTGGATGTCCTAAAGTTCTACGACTCCAACACAGTGAAGCAGAAATA
TCTGAGCTTTACTCCTCCTGAGAAAGATGGCTTTCCTTCTGGAACACCAG
CACTGAATGCCAAGGGAACAGAAGCACCCGCAGTAGTGACAGAGGAGGAG
GATGATGATGAAGAGACTGCTCCTCCCGTTATTGCCCCGCGACCGGATCA
TACGAAATCAATTTACACACGGTCTGTAATTGACCCTGTTCCTGCACCAG
TTGGTGATTCACATGTTGATGGTGCTGCCAAGTCTTTAGACAAACAGAAA
AAGAAGACTAAGATGACAGATGAAGAGATTATGGAGAAATTAAGAACTAT
CGTGAGCATAGGTGACCCTAAGAAAAAATATACAAGATATGAAAAAATTG
GACAAAGGGCTTCTGGTACAGTTTTCACTGCTACTGACGTTGCACTGGGA
CAGGAGGTTGCTATCAAACAAATTAATTTACAGAAACAGCCAAAGAAGGA
ACTGATCATTAACGAGATTCTGGTGATGAAAGAATTGAAAAATCCCAACA
TCGTTAACTTTTTGGACAGTTACCTGGTAGGAGATGAATTGTTTGTGGTC
ATGGAATACCTTGCTGGGGGGTCACTCACTGATGTGGTAACAGAAACGTG
CATGGATGAAGCACAGATTGCTGCTGTATGCAGAGAGTGTTTACAGGCAT
TGGAGTTTTTACATGCTAATCAAGTGATCCACAGAGACATCAAAAGTGAC
AATGTACTTTTGGGAATGGAAGGATCTGTTAAGCTCACTGACTTTGGTTT
CTGTGCCCAGATCACCCCTGAGCAGAGCAAACGCAGTGAGATGGTCGGAA
CGCCATACTGGATGGCACCAGAGGTGGTTACACGGAAAGCTTATGGCCCT
AAAGTCGACATATGGTCTCTGGGTATCATGGCTATTGAGATGGTAGAAGG
AGAGCCTCCATACCTCAATGAAAATCCCTTGAGGGCCTTGTACCTAATAG
CAACTAATGGAACCCCAGAACTTCAGAATCCAGAGAAACTTTCCCCAATA
TTTCGGGATTTCTTAAATCGATGTTTGGAAATGGATGTGGAAAAAAGGGG
TTCAGCCAAAGAATTATTACAGCATCCTTTCCTGAAACTGGCCAAACCGT
TATCTAGCTTGACACCACTGATCATGGCAGCTAAAGAAGCAATGAAGAGT
AACCGTTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMSDNGELEDKPPAPPVRMSSTI
FSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKGSKKKEKERP
EISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQA
VLDVLKFYDSNTVKQKYLSFTPPEKDGFPSGTPALNAKGTEAPAVVTEEE
DDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSHVDGAAKSLDKQK
KKTKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQRASGTVFTATDVALG
QEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVV
MEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSD
NVLLGMEGSVKLTDFGFCAQITPEQSKRSEMVGTPYWMAPEVVTRKAYGP
KVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPI
FRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKS
NR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4819
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6H.; Note: This sequence differs from the genomic sequence of PAK2 because there is a G258R mutation in this particular accession.