cDNA Clone PfSUB-1

• Plasmid: pFB1 PfSUB-1
• Parent Plasmid: pFastBac HTb
• Expressed: PfSUB-1
• Genbank: AJ242589
• Species: Plasmodium falciparum

Sequence of Insert

CGGGGGCGCGGATCCGAAACCATGATGCTGAACAAGAAGGTGGTGGCCTT
GTGTACTTTGACTTTGCACTTGTTCTGTATCTTCCTGTGTTTGGGTAAGG
AGGTACGTAGCGAGGAGAACGGAAAGATCCAAGACGACGCTAAGAAGATC
GTGTCTGAGCTGAGATTCTTGGAGAAGGTTGAAGACGTGATCGAGAAGTC
CAACATCGGAGGTAACGAAGTTGACGCTGACGAGAACTCGTTCAACCCGG
ACACTGAAGTCCCAATCGAAGAGATTGAGGAGATCAAGATGAGAGAGCTG
AAGGACGTCAAGGAGGAGAAGAACAAGAACGATAACCACAACAACAACAA
TAACAACAACAACATCTCGTCTTCCTCAAGTTCCTCTTCTAACACTTTCG
GAGAGGAGAAGGAGGAGGTCTCGAAGAAGAAGAAGAAGCTGAGATTGATC
GTGTCTGAGAACCATGCCACTACTCCATCTTTCTTCCAAGAGTCTCTGTT
GGAGCCCGACGTGCTGTCCTTCTTGGAGTCTAAGGGAAACTTGTCTAACC
TGAAGAACATCAACTCCATGATCATCGAGCTGAAGGAGGATACCACTGAT
GATGAGTTGATCTCGTACATCAAGATCTTGGAAGAGAAGGGTGCTTTGAT
CGAGTCTGACAAGTTGGTGTCCGCTGATAACATCGACATCTCTGGTATCA
AGGATGCCATCAGACGTGGAGAGGAGAACATCGATGTCAACGATTACAAG
TCCATGCTGGAGGTCGAGAACGATGCTGAGGACTACGACAAGATGTTCGG
AATGTTCAACGAGTCTCACGCAGCTACTTCTAAGCGCAAGAGACACTCCA
CTAACGAGAGAGGATACGATACCTTCTCCAGCCCAAGCTACAAGACTTAC
TCCAAGTCTGATTACTTGTACGATGATGACAACAATAATAACAACTACTA
CTACTCCCATTCGTCTAACGGCCATAACTCCTCCTCTCGTAACTCGTCTT
CCTCTAGATCCAGACCCGGTAAGTACCACTTCAACGATGAGTTCAGAAAC
CTGCAATGGGGTCTGGACCTGTCTCGTTTGGACGAGACCCAAGAGTTGAT
CAACGAGCACCAGGTCATGAGTACCAGAATCTGTGTCATCGACTCTGGCA
TCGACTACAACCATCCAGATTTGAAGGACAACATCGAGTTGAACCTGAAG
GAGTTGCACGGTCGTAAGGGTTTCGATGACGATAACAACGGCATCGTGGA
CGACATCTACGGTGCTAACTTCGTGAACAACTCTGGCAACCCCATGGATG
ATAACTACCACGGAACTCATGTCTCTGGAATCATCTCCGCTATCGGAAAC
AATAACATCGGAGTGGTCGGAGTGGATGTCAACTCCAAGTTGATCATCTG
TAAGGCCCTGGATGAGCATAAGCTGGGAAGATTGGGAGATATGTTCAAGT
GTTTGGACTACTGTATCTCGCGTAACGCTCATATGATCAACGGTTCCTTC
TCTTTCGACGAATACTCCGGTATCTTCAACTCTTCTGTCGAGTACCTGCA
AAGAAAGGGTATCTTGTTCTTCGTGTCTGCGTCGAACTGTTCCCATCCAA
AGTCTAGCACCCCAGATATCCGCAAGTGTGACCTGTCTATCAACGCTAAG
TACCCACCCATCTTGTCTACTGTCTACGACAACGTTATCTCTGTGGCTAA
CTTGAAGAAGAACGATAATAACAACCACTACTCTCTGTCGATCAACTCTT
TCTACTCCAACAAGTACTGTCAATTGGCAGCTCCAGGTACTAACATCTAC
TCGACCGCTCCACATAACTCTTACAGAAAGTTGAACGGTACCTCTATGGC
TGCTCCACATGTTGCTGCCATCGCTAGCTTGATCTTCTCAATCAACCCAG
ACCTGTCCTACAAGAAGGTCATCCAGATCCTTAAGGATTCCATCGTGTAC
TTGCCATCCTTGAAGAACATGGTTGCTTGGGCTGGTTACGCTGACATCAA
CAAGGCTGTCAACTTGGCTATCAAGTCCAAGAAGACGTACATCAACTCTA
ACATCTCCAACAAGTGGAAGAAGAAGTCCAGATACCTGCACCACCACCAT
CACCATTAGGCGGCCGCTTTCGAATCTAGAGCCTGCAGTCTCGAGGCATG
CGTAACCAAAAGCCTGTCCA

Amino Acid Sequence

MMLNKKVVALCTLTLHLFCIFLCLGKEVRSEENGKIQDDAKKIVSELRFL
EKVEDVIEKSNIGGNEVDADENSFNPDTEVPIEEIEEIKMRELKDVKEEK
NKNDNHNNNNNNNNISSSSSSSSNTFGEEKEEVSKKKKKLRLIVSENHAT
TPSFFQESLLEPDVLSFLESKGNLSNLKNINSMIIELKEDTTDDELISYI
KILEEKGALIESDKLVSADNIDISGIKDAIRRGEENIDVNDYKSMLEVEN
DAEDYDKMFGMFNESHAATSKRKRHSTNERGYDTFSSPSYKTYSKSDYLY
DDDNNNNNYYYSHSSNGHNSSSRNSSSSRSRPGKYHFNDEFRNLQWGLDL
SRLDETQELINEHQVMSTRICVIDSGIDYNHPDLKDNIELNLKELHGRKG
FDDDNNGIVDDIYGANFVNNSGNPMDDNYHGTHVSGIISAIGNNNIGVVG
VDVNSKLIICKALDEHKLGRLGDMFKCLDYCISRNAHMINGSFSFDEYSG
IFNSSVEYLQRKGILFFVSASNCSHPKSSTPDIRKCDLSINAKYPPILST
VYDNVISVANLKKNDNNNHYSLSINSFYSNKYCQLAAPGTNIYSTAPHNS
YRKLNGTSMAAPHVAAIASLIFSINPDLSYKKVIQILKDSIVYLPSLKNM
VAWAGYADINKAVNLAIKSKKTYINSNISNKWKKKSRYLHHHHHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4799
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H tag.