cDNA Clone EWS

• Plasmid: pCG4 EWS
• Parent Plasmid: pCG4
• Expressed: EWS
• Genbank: NP_005234
• Species: Human

Sequence of Insert

ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAA
AAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGA
ACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACT
AGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCT
ATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGG
ATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGA
TATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGG
AAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGAATTC
GCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTA
CAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGG
CATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTGATGTCAGC
TATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAAC
TTCTTATGGACAGCCTCCCACTGGTTATACTACTCCAACTGCCCCCCAGG
CATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACC
ACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAGTCTGCATA
TGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTG
CACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCT
CAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAG
TAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCA
CTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACT
AGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAG
CAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGC
AGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCA
AGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTCATTCCG
ACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAGGAGTCTGGAGGAT
TTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAACCGGGGC
AGGGGAAGAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCGGGG
AGGAGGACGCGGTGGAATGGGCAGCGCTGGAGAGCGAGGTGGCTTCAATA
AGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGCCCTCCT
GTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACAAGGATT
AAATGACAGTGTGACTCTAGATGATCTGGCAGACTTCTTTAAGCAGTGTG
GGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCATGATCCACATCTAC
CTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTCCTATGA
AGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATT
TTCAAGGGAGCAAACTTAAAGTCTCCCTTGCTCGGAAGAAGCCTCCAATG
AACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCATGCCACC
ACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGACCCATGG
GTCGCATGGGAGGCCGTGGAGGAGATAGAGGAGGCTTCCCTCCAAGAGGA
CCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAAACGTCCAGCACCG
AGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGAACTTCG
CCTGGAGAACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAAGGCTTC
CTCCCGCCACCCTTTCCGCCCCCGGGTGGTGATCGTGGCAGAGGTGGCCC
TGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTGGTCCCG
GTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGTGGT
GGCCGGGGCATGGACCGAGGTGGCTTTGGTGGAGGAAGACGAGGTGGCCC
TGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGAGGAAGAAGAGGAG
GACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAGGAGCGC
AGAGATCGGCCCTACTAG

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEF
ASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVS
YTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDTT
TATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQP
QSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPT
SYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAP
SQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRG
RGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPP
VDPDEDSDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIY
LDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPM
NSMRGGLPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRG
PRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGF
LPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRG
GRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQER
RDRPY*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4789
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Y2H construct with N term GAL4 DNA-binding domain.