cDNA Clone EWS

• Plasmid: pCG4 EWS 2-265 T79D
• Parent Plasmid: pCG4
• Expressed: EWS 2-265 T79D
• Genbank: NP_005234
• Species: Human

Sequence of Insert

ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAA
AAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGA
ACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACT
AGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCT
ATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGG
ATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGA
TATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGG
AAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGAATTC
GCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAGCAGGGCTA
CAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGG
CATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTGATGTCAGC
TATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAAC
TTCTTATGGACAGCCTCCCACTGGTTATACTGATCCAACTGCCCCCCAGG
CATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACC
ACTGCTACAGTCACCACCACCCAAGCCTCCTATGCAGCTCAGTCTGCATA
TGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTG
CACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTAGTCAACCT
CAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAG
TAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCAGCCAGTCA
CTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACT
AGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGGCAACCGAG
CAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGC
AGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCCAAGCTCCA
AGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGTAG

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEF
ASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVS
YTQAQTTATYGQTAYATSYGQPPTGYTDPTAPQAYSQPVQGYGTGAYDTT
TATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQP
QSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPT
SYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAP
SQYSQQSSSYGQQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4784
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Y2H construct with N term GAL4 DNA-binding domain.