cDNA Clone MHC2TA

• Plasmid: pCMV5-HA-2 MHC2TA with card domain
• Parent Plasmid: pCMV5
• Expressed: HA-MHC2TA with card domain
• Genbank: BAE25629
• Tag: HA
• Species: Mouse

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGAACCA
CTTCCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAGC
CCAGGCAGGTGCGGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCTC
TCACGGGAATACCACTGTGCCTTGCTGCATGAGCCTGATGGTGATGCCCT
GGCCCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTGA
CTTTCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAGG
GGCACCAGCTACAGGGACCATGGAGACCATAGTCTGTGTGCCACCATGGA
TCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAGTGATGCCG
ACCCCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGAG
GAGGAGATCGAACTCAGCTCAGAGCCAGACACAGATACCATCAACTGCGA
CCAGTTCAGCAAGCTGTTGCAGGACATGGAACTGGATGAAGAGACCCGGG
AGGCCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACC
CAGCTCGAGGGCCTGAGCAAGGACCTCTTCATAGAGCACATTGGAGCAGA
GGAAGGCTTTGGTGAGAACATAGAGATCCCTGTAGAAGCAGGACAGAAGC
CTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGCACAGG
AAGCTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCAC
TGGGCACATCCAGATCTTCACCACTCTGCCCCAGGGACTCTGGCAAATCT
CAGGGGCTGGCACAGGTCTCTCCAGTGTCCTAATCTACCACGGTGAGATG
CCCCAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCT
CCCCGAGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCTG
CAGCTGACCTGCCCAGCATGCCCGAACCTGCGCTGACCTCCCGTGTAAAT
GAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAGTCTTC
CATCAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCC
TACAGGACAAATACAAGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGTG
GCCGTGGAGCTGGTACGGGCCAGGCTGGAAAGAGGCAGCAACAAGAGCCA
GGAAAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACG
GTGGTCTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGGA
GAGACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGCCAGGGAAAGAGCCA
CTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCTACAAT
ATGACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGAC
ACCTACCACCTGCGGGATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGGC
CATGGATGACGAGGTCCTTGATTATATCGTGAGGCAGCCAGACCGTGTTC
TGCTCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTG
CACGGACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGACT
GCTGGCTGGGATCTTCCAGCGGAAGCTACTGCGAGGCTGCACACTGCTCC
TCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGAT
GCCATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACAT
GAGGCACTACTTTGAGAACTCAGGGACAGCGGGGAACCAAGACAAGGCCC
TGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGCTATAGTCACAGCCCT
GTTGTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGG
CACAGAGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTGC
TAGGTCCTGCAGCTCAGAACAGTCCTCCCGGAGCCTTAGTCGAGCTGGCC
AAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAAGAAAC
CCGGTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGA
TGCAGCAGACCCTGGAGACCACGGAGGCTCAACTGGCCTTCTCCAGTTTT
CTGCTACAGTGTTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAAAT
CAAAGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGA
GACCCTATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATTA
GTTTTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGCCTGCAGT
GGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCTGAAGC
GCCTGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCAC
TGTGCCCACGAGACACAGCAACCTGGGATATGGGAGCATGTTGCACACCA
GCTCCCTGGCCACCTCTCCTTCCTGGGCACCCGGCTCACACCCCCAGATG
TGTATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTG
GACCTTCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGGG
ACTCAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGATACAATGGCAT
TATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGGCGGCA
GAGGAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGT
GGAAGACCTGGATCGTCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCAG
AAGATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGAG
TTTGCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAA
GATCCTTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTTA
GTGAGAACAAGATCGGAGACAAGGGTGTGTCGGAGCTCTCAGCCACCTTC
CCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAACATCAC
TGATGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGT
CCCTCCTAAGGCTGAGCTTGTACAATAACTGCATCTGTGACAAAGGAGCC
AAGAGCCTGGCACAAGTACTTCCGGACATGGTGTCCCTGCGTGTGATGGA
TGTCCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCA
GCCTTCAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCACT
ATCCCCTTTGGGGTTCAGGAACACCTGCAGCAGCTGGATGCCAGGATCAG
TCTGAGATGAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSMNHFQAILAQVQTLLSSQKPRQVRALLDGLLEEELLSR
EYHCALLHEPDGDALARKISLTLLEKGDLDLTFLSWVCNSLQAPTVERGT
SYRDHGDHSLCATMDLGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEE
IELSSEPDTDTINCDQFSKLLQDMELDEETREAYANIAELDQYVFQDTQL
EGLSKDLFIEHIGAEEGFGENIEIPVEAGQKPQKRRFPEEHAMDSKHRKL
VPTSRTSLNYLDLPTGHIQIFTTLPQGLWQISGAGTGLSSVLIYHGEMPQ
VNQVLPSSSLSIPSLPESPDRPGSTSPFTPSAADLPSMPEPALTSRVNET
EDTSPSPCQEGPESSIKLPKWPEAVERFQHSLQDKYKALPQSPRGPLVAV
ELVRARLERGSNKSQERELATPDWTERQLAHGGLAEVLQVVSDCRRPGET
QVVAVLGKAGQGKSHWARTVSHTWACGQLLQYDFVFYVPCHCLDRPGDTY
HLRDLLCPPSLQPLAMDDEVLDYIVRQPDRVLLILDAFEELEAQDGLLHG
PCGSLSPEPCSLRGLLAGIFQRKLLRGCTLLLTARPRGRLAQSLSKADAI
FEVPSFSTKQAKTYMRHYFENSGTAGNQDKALGLLEGQPLLCSYSHSPVV
CRAVCQLSKALLEQGTEAQLPCTLTGLYVSLLGPAAQNSPPGALVELAKL
AWELGRRHQSTLQETRFSSVEVKTWAVTQGLMQQTLETTEAQLAFSSFLL
QCFLGAVWLAQCNEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLVF
QPRAHCLGALVEPAVAAVADRKQKVLTRYLKRLKLGTLRAGRLLELLHCA
HETQQPGIWEHVAHQLPGHLSFLGTRLTPPDVYVLGRALETASQDFSLDL
RQTGVEPSGLGNLVGLSCVTSFRASLSDTMALWESLQQQGEAQLLQAAEE
KFTIEPFKAKSPKDVEDLDRLVQTQRLRNPSEDAAKDLPAIRDLKKLEFA
LGPILGPQAFPTLAKILPAFSSLQHLDLDSLSENKIGDKGVSELSATFPQ
LKALETLNLSQNNITDVGACKLAEALPALAKSLLRLSLYNNCICDKGAKS
LAQVLPDMVSLRVMDVQFNKFTAAGAQQLASSLQKCPQVETLAMWTPTIP
FGVQEHLQQLDARISLR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4783
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA.