cDNA Clone MHC2TA

• Plasmid: pGEX6P-2-MHC2TA with card domain
• Parent Plasmid: pGEX6P2
• Expressed: GST-MHC2TA with card domain
• Genbank: BAE25629
• Tag: GST
• Species: Mouse

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGAACC
ACTTCCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAG
CCCAGGCAGGTGCGGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCT
CTCACGGGAATACCACTGTGCCTTGCTGCATGAGCCTGATGGTGATGCCC
TGGCCCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTG
ACTTTCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAG
GGGCACCAGCTACAGGGACCATGGAGACCATAGTCTGTGTGCCACCATGG
ATCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAGTGATGCC
GACCCCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGA
GGAGGAGATCGAACTCAGCTCAGAGCCAGACACAGATACCATCAACTGCG
ACCAGTTCAGCAAGCTGTTGCAGGACATGGAACTGGATGAAGAGACCCGG
GAGGCCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATAC
CCAGCTCGAGGGCCTGAGCAAGGACCTCTTCATAGAGCACATTGGAGCAG
AGGAAGGCTTTGGTGAGAACATAGAGATCCCTGTAGAAGCAGGACAGAAG
CCTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGCACAG
GAAGCTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCA
CTGGGCACATCCAGATCTTCACCACTCTGCCCCAGGGACTCTGGCAAATC
TCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTAATCTACCACGGTGAGAT
GCCCCAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTC
TCCCCGAGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCT
GCAGCTGACCTGCCCAGCATGCCCGAACCTGCGCTGACCTCCCGTGTAAA
TGAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAGTCTT
CCATCAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCC
CTACAGGACAAATACAAGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGT
GGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAGAGGCAGCAACAAGAGCC
AGGAAAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCAC
GGTGGTCTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGG
AGAGACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGCCAGGGAAAGAGCC
ACTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCTACAA
TATGACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGA
CACCTACCACCTGCGGGATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGG
CCATGGATGACGAGGTCCTTGATTATATCGTGAGGCAGCCAGACCGTGTT
CTGCTCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCT
GCACGGACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGAC
TGCTGGCTGGGATCTTCCAGCGGAAGCTACTGCGAGGCTGCACACTGCTC
CTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGA
TGCCATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACA
TGAGGCACTACTTTGAGAACTCAGGGACAGCGGGGAACCAAGACAAGGCC
CTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGCTATAGTCACAGCCC
TGTTGTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGG
GCACAGAGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTG
CTAGGTCCTGCAGCTCAGAACAGTCCTCCCGGAGCCTTAGTCGAGCTGGC
CAAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAAGAAA
CCCGGTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTG
ATGCAGCAGACCCTGGAGACCACGGAGGCTCAACTGGCCTTCTCCAGTTT
TCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAAA
TCAAAGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAG
AGACCCTATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATT
AGTTTTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGCCTGCAG
TGGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCTGAAG
CGCCTGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCA
CTGTGCCCACGAGACACAGCAACCTGGGATATGGGAGCATGTTGCACACC
AGCTCCCTGGCCACCTCTCCTTCCTGGGCACCCGGCTCACACCCCCAGAT
GTGTATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTT
GGACCTTCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGG
GACTCAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGATACAATGGCA
TTATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGGCGGC
AGAGGAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATG
TGGAAGACCTGGATCGTCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCA
GAAGATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGA
GTTTGCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAA
AGATCCTTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTT
AGTGAGAACAAGATCGGAGACAAGGGTGTGTCGGAGCTCTCAGCCACCTT
CCCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAACATCA
CTGATGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAG
TCCCTCCTAAGGCTGAGCTTGTACAATAACTGCATCTGTGACAAAGGAGC
CAAGAGCCTGGCACAAGTACTTCCGGACATGGTGTCCCTGCGTGTGATGG
ATGTCCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCC
AGCCTTCAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCAC
TATCCCCTTTGGGGTTCAGGAACACCTGCAGCAGCTGGATGCCAGGATCA
GTCTGAGATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNHFQAILAQVQTLLSSQK
PRQVRALLDGLLEEELLSREYHCALLHEPDGDALARKISLTLLEKGDLDL
TFLSWVCNSLQAPTVERGTSYRDHGDHSLCATMDLGSPEGSYLELLNSDA
DPLHLYHLYDQMDLAGEEEIELSSEPDTDTINCDQFSKLLQDMELDEETR
EAYANIAELDQYVFQDTQLEGLSKDLFIEHIGAEEGFGENIEIPVEAGQK
PQKRRFPEEHAMDSKHRKLVPTSRTSLNYLDLPTGHIQIFTTLPQGLWQI
SGAGTGLSSVLIYHGEMPQVNQVLPSSSLSIPSLPESPDRPGSTSPFTPS
AADLPSMPEPALTSRVNETEDTSPSPCQEGPESSIKLPKWPEAVERFQHS
LQDKYKALPQSPRGPLVAVELVRARLERGSNKSQERELATPDWTERQLAH
GGLAEVLQVVSDCRRPGETQVVAVLGKAGQGKSHWARTVSHTWACGQLLQ
YDFVFYVPCHCLDRPGDTYHLRDLLCPPSLQPLAMDDEVLDYIVRQPDRV
LLILDAFEELEAQDGLLHGPCGSLSPEPCSLRGLLAGIFQRKLLRGCTLL
LTARPRGRLAQSLSKADAIFEVPSFSTKQAKTYMRHYFENSGTAGNQDKA
LGLLEGQPLLCSYSHSPVVCRAVCQLSKALLEQGTEAQLPCTLTGLYVSL
LGPAAQNSPPGALVELAKLAWELGRRHQSTLQETRFSSVEVKTWAVTQGL
MQQTLETTEAQLAFSSFLLQCFLGAVWLAQCNEIKDKELPQYLALTPRKK
RPYDNWLEGVPRFLAGLVFQPRAHCLGALVEPAVAAVADRKQKVLTRYLK
RLKLGTLRAGRLLELLHCAHETQQPGIWEHVAHQLPGHLSFLGTRLTPPD
VYVLGRALETASQDFSLDLRQTGVEPSGLGNLVGLSCVTSFRASLSDTMA
LWESLQQQGEAQLLQAAEEKFTIEPFKAKSPKDVEDLDRLVQTQRLRNPS
EDAAKDLPAIRDLKKLEFALGPILGPQAFPTLAKILPAFSSLQHLDLDSL
SENKIGDKGVSELSATFPQLKALETLNLSQNNITDVGACKLAEALPALAK
SLLRLSLYNNCICDKGAKSLAQVLPDMVSLRVMDVQFNKFTAAGAQQLAS
SLQKCPQVETLAMWTPTIPFGVQEHLQQLDARISLR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4782
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.