cDNA Clone MHC2TA

• Plasmid: pCMV5-FLAG-2 MHC2TA with card domain
• Parent Plasmid: pCMV5
• Expressed: FLAG-MHC2TA with card domain
• Genbank: BAE25629
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCATGAACCACTT
CCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAGCCCA
GGCAGGTGCGGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCTCTCA
CGGGAATACCACTGTGCCTTGCTGCATGAGCCTGATGGTGATGCCCTGGC
CCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTGACTT
TCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAGGGGC
ACCAGCTACAGGGACCATGGAGACCATAGTCTGTGTGCCACCATGGATCT
GGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAGTGATGCCGACC
CCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGAGGAG
GAGATCGAACTCAGCTCAGAGCCAGACACAGATACCATCAACTGCGACCA
GTTCAGCAAGCTGTTGCAGGACATGGAACTGGATGAAGAGACCCGGGAGG
CCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCAG
CTCGAGGGCCTGAGCAAGGACCTCTTCATAGAGCACATTGGAGCAGAGGA
AGGCTTTGGTGAGAACATAGAGATCCCTGTAGAAGCAGGACAGAAGCCTC
AGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGCACAGGAAG
CTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCACTGG
GCACATCCAGATCTTCACCACTCTGCCCCAGGGACTCTGGCAAATCTCAG
GGGCTGGCACAGGTCTCTCCAGTGTCCTAATCTACCACGGTGAGATGCCC
CAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCCC
CGAGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCTGCAG
CTGACCTGCCCAGCATGCCCGAACCTGCGCTGACCTCCCGTGTAAATGAG
ACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAGTCTTCCAT
CAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCCTAC
AGGACAAATACAAGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGTGGCC
GTGGAGCTGGTACGGGCCAGGCTGGAAAGAGGCAGCAACAAGAGCCAGGA
AAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGTG
GTCTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGGAGAG
ACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGCCAGGGAAAGAGCCACTG
GGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCTACAATATG
ACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGACACC
TACCACCTGCGGGATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGGCCAT
GGATGACGAGGTCCTTGATTATATCGTGAGGCAGCCAGACCGTGTTCTGC
TCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCAC
GGACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGACTGCT
GGCTGGGATCTTCCAGCGGAAGCTACTGCGAGGCTGCACACTGCTCCTCA
CAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGATGCC
ATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACATGAG
GCACTACTTTGAGAACTCAGGGACAGCGGGGAACCAAGACAAGGCCCTGG
GCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGCTATAGTCACAGCCCTGTT
GTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCAC
AGAGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTGCTAG
GTCCTGCAGCTCAGAACAGTCCTCCCGGAGCCTTAGTCGAGCTGGCCAAG
CTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAAGAAACCCG
GTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGATGC
AGCAGACCCTGGAGACCACGGAGGCTCAACTGGCCTTCTCCAGTTTTCTG
CTACAGTGTTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAAATCAA
AGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGAC
CCTATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATTAGTT
TTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGCCTGCAGTGGC
TGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCTGAAGCGCC
TGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCACTGT
GCCCACGAGACACAGCAACCTGGGATATGGGAGCATGTTGCACACCAGCT
CCCTGGCCACCTCTCCTTCCTGGGCACCCGGCTCACACCCCCAGATGTGT
ATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGAC
CTTCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGGGACT
CAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGATACAATGGCATTAT
GGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGGCGGCAGAG
GAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGTGGA
AGACCTGGATCGTCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCAGAAG
ATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGAGTTT
GCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGAT
CCTTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTTAGTG
AGAACAAGATCGGAGACAAGGGTGTGTCGGAGCTCTCAGCCACCTTCCCT
CAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAACATCACTGA
TGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGTCCC
TCCTAAGGCTGAGCTTGTACAATAACTGCATCTGTGACAAAGGAGCCAAG
AGCCTGGCACAAGTACTTCCGGACATGGTGTCCCTGCGTGTGATGGATGT
CCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGCC
TTCAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCACTATC
CCCTTTGGGGTTCAGGAACACCTGCAGCAGCTGGATGCCAGGATCAGTCT
GAGATGAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMNHFQAILAQVQTLLSSQKPRQVRALLDGLLEEELLSRE
YHCALLHEPDGDALARKISLTLLEKGDLDLTFLSWVCNSLQAPTVERGTS
YRDHGDHSLCATMDLGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEEI
ELSSEPDTDTINCDQFSKLLQDMELDEETREAYANIAELDQYVFQDTQLE
GLSKDLFIEHIGAEEGFGENIEIPVEAGQKPQKRRFPEEHAMDSKHRKLV
PTSRTSLNYLDLPTGHIQIFTTLPQGLWQISGAGTGLSSVLIYHGEMPQV
NQVLPSSSLSIPSLPESPDRPGSTSPFTPSAADLPSMPEPALTSRVNETE
DTSPSPCQEGPESSIKLPKWPEAVERFQHSLQDKYKALPQSPRGPLVAVE
LVRARLERGSNKSQERELATPDWTERQLAHGGLAEVLQVVSDCRRPGETQ
VVAVLGKAGQGKSHWARTVSHTWACGQLLQYDFVFYVPCHCLDRPGDTYH
LRDLLCPPSLQPLAMDDEVLDYIVRQPDRVLLILDAFEELEAQDGLLHGP
CGSLSPEPCSLRGLLAGIFQRKLLRGCTLLLTARPRGRLAQSLSKADAIF
EVPSFSTKQAKTYMRHYFENSGTAGNQDKALGLLEGQPLLCSYSHSPVVC
RAVCQLSKALLEQGTEAQLPCTLTGLYVSLLGPAAQNSPPGALVELAKLA
WELGRRHQSTLQETRFSSVEVKTWAVTQGLMQQTLETTEAQLAFSSFLLQ
CFLGAVWLAQCNEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLVFQ
PRAHCLGALVEPAVAAVADRKQKVLTRYLKRLKLGTLRAGRLLELLHCAH
ETQQPGIWEHVAHQLPGHLSFLGTRLTPPDVYVLGRALETASQDFSLDLR
QTGVEPSGLGNLVGLSCVTSFRASLSDTMALWESLQQQGEAQLLQAAEEK
FTIEPFKAKSPKDVEDLDRLVQTQRLRNPSEDAAKDLPAIRDLKKLEFAL
GPILGPQAFPTLAKILPAFSSLQHLDLDSLSENKIGDKGVSELSATFPQL
KALETLNLSQNNITDVGACKLAEALPALAKSLLRLSLYNNCICDKGAKSL
AQVLPDMVSLRVMDVQFNKFTAAGAQQLASSLQKCPQVETLAMWTPTIPF
GVQEHLQQLDARISLR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4781
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG.