cDNA Clone MHC2TA

• Plasmid: pFBHTa MHC2TA
• Parent Plasmid: pSC-A
• Expressed: His-MHC2TA
• Genbank: NM_000246
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGATCCGGAATTCATGCGTTGCC
TGGCTCCACGCCCTGCTGGGTCCTACCTGTCAGAGCCCCAAGGCAGCTCA
CAGTGTGCCACCATGGAGTTGGGGCCCCTAGAAGGTGGCTACCTGGAGCT
TCTTAACAGCGATGCTGACCCCCTGTGCCTCTACCACTTCTATGACCAGA
TGGACCTGGCTGGAGAAGAAGAGATTGAGCTCTACTCAGAACCCGACACA
GACACCATCAACTGCGACCAGTTCAGCAGGCTGTTGTGTGACATGGAAGG
TGATGAAGAGACCAGGGAGGCTTATGCCAATATCGCGGAACTGGACCAGT
ATGTCTTCCAGGACTCCCAGCTGGAGGGCCTGAGCAAGGACATTTTCAAG
CACATAGGACCAGATGAAGTGATCGGTGAGAGTATGGAGATGCCAGCAGA
AGTTGGGCAGAAAAGTCAGAAAAGACCCTTCCCAGAGGAGCTTCCGGCAG
ACCTGAAGCACTGGAAGCCAGCTGAGCCCCCCACTGTGGTGACTGGCAGT
CTCCTAGTGGGACCAGTGAGCGACTGCTCCACCCTGCCCTGCCTGCCACT
GCCTGCGCTGTTCAACCAGGAGCCAGCCTCCGGCCAGATGCGCCTGGAGA
AAACCGACCAGATTCCCATGCCTTTCTCCAGTTCCTCGTTGAGCTGCCTG
AATCTCCCTGAGGGACCCATCCAGTTTGTCCCCACCATCTCCACTCTGCC
CCATGGGCTCTGGCAAATCTCTGAGGCTGGAACAGGGGTCTCCAGTATAT
TCATCTACCATGGTGAGGTGCCCCAGGCCAGCCAAGTACCCCCTCCCAGT
GGATTCACTGTCCACGGCCTCCCAACATCTCCAGACCGGCCAGGCTCCAC
CAGCCCCTTCGCTCCATCAGCCACTGACCTGCCCAGCATGCCTGAACCTG
CCCTGACCTCCCGAGCAAACATGACAGAGCACAAGACGTCCCCCACCCAA
TGCCCGGCAGCTGGAGAGGTCTCCAACAAGCTTCCAAAATGGCCTGAGCC
GGTGGAGCAGTTCTACCGCTCACTGCAGGACACGTATGGTGCCGAGCCCG
CAGGCCCGGATGGCATCCTAGTGGAGGTGGATCTGGTGCAGGCCAGGCTG
GAGAGGAGCAGCAGCAAGAGCCTGGAGCGGGAACTGGCCACCCCGGACTG
GGCAGAACGGCAGCTGGCCCAAGGAGGCCTGGCTGAGGTGCTGTTGGCTG
CCAAGGAGCACCGGCGGCCGCGTGAGACACGAGTGATTGCTGTGCTGGGC
AAAGCTGGTCAGGGCAAGAGCTATTGGGCTGGGGCAGTGAGCCGGGCCTG
GGCTTGTGGCCGGCTTCCCCAGTACGACTTTGTCTTCTCTGTCCCCTGCC
ATTGCTTGAACCGTCCGGGGGATGCCTATGGCCTGCAGGATCTGCTCTTC
TCCCTGGGCCCACAGCCACTCGTGGCGGCCGATGAGGTTTTCAGCCACAT
CTTGAAGAGACCTGACCGCGTTCTGCTCATCCTAGACGCCTTCGAGGAGC
TGGAAGCGCAAGATGGCTTCCTGCACAGCACGTGCGGACCGGCACCGGCG
GAGCCCTGCTCCCTCCGGGGGCTGCTGGCCGGCCTTTTCCAGAAGAAGCT
GCTCCGAGGTTGCACCCTCCTCCTCACAGCCCGGCCCCGGGGCCGCCTGG
TCCAGAGCCTGAGCAAGGCCGACGCCCTATTTGAGCTGTCCGGCTTCTCC
ATGGAGCAGGCCCAGGCATACGTGATGCGCTACTTTGAGAGCTCAGGGAT
GACAGAGCACCAAGACAGAGCCCTGACGCTCCTCCGGGACCGGCCACTTC
TTCTCAGTCACAGCCACAGCCCTACTTTGTGCCGGGCAGTGTGCCAGCTC
TCAGAGGCCCTGCTGGAGCTTGGGGAGGACGCCAAGCTGCCCTCCACGCT
CACGGGACTCTATGTCGGCCTGCTGGGCCGTGCAGCCCTCGACAGCCCCC
CCGGGGCCCTGGCAGAGCTGGCCAAGCTGGCCTGGGAGCTGGGCCGCAGA
CATCAAAGTACCCTACAGGAGGACCAGTTCCCATCCGCAGACGTGAGGAC
CTGGGCGATGGCCAAAGGCTTAGTCCAACACCCACCGCGGGCCGCAGAGT
CCGAGCTGGCCTTCCCCAGCTTCCTCCTGCAATGCTTCCTGGGGGCCCTG
TGGCTGGCTCTGAGTGGCGAAATCAAGGACAAGGAGCTCCCGCAGTACCT
AGCATTGACCCCAAGGAAGAAGAGGCCCTATGACAACTGGCTGGAGGGCG
TGCCACGCTTTCTGGCTGGGCTGATCTTCCAGCCTCCCGCCCGCTGCCTG
GGAGCCCTACTCGGGCCATCGGCGGCTGCCTCGGTGGACAGGAAGCAGAA
GGTGCTTGCGAGGTACCTGAAGCGGCTGCAGCCGGGGACACTGCGGGCGC
GGCAGCTGCTTGAGCTGCTGCACTGCGCCCACGAGGCCGAGGAGGCTGGA
ATTTGGCAGCACGTGGTACAGGAGCTCCCCGGCCGCCTCTCTTTTCTGGG
CACCCGCCTCACGCCTCCTGATGCACATGTACTGGGCAAGGCCTTGGAGG
CGGCGGGCCAAGACTTCTCCCTGGACCTCCGCAGCACTGGCATTTGCCCC
TCTGGATTGGGGAGCCTCGTGGGACTCAGCTGTGTCACCCGTTTCAGGGC
TGCCTTGAGCGACACGGTGGCGCTGTGGGAGTCCCTGCGGCAGCATGGGG
AGACCAAGCTACTTCAGGCAGCAGAGGAGAAGTTCACCATCGAGCCTTTC
AAAGCCAAGTCCCTGAAGGATGTGGAAGACCTGGGAAAGCTTGTGCAGAC
TCAGAGGACGAGAAGTTCCTCGGAAGACACAGCTGGGGAGCTCCCTGCTG
TTCGGGACCTAAAGAAACTGGAGTTTGCGCTGGGCCCTGTCTCAGGCCCC
CAGGCTTTCCCCAAACTGGTGCGGATCCTCACGGCCTTTTCCTCCCTGCA
GCATCTGGACCTGGATGCGCTGAGTGAGAACAAGATCGGGGACGAGGGTG
TCTCGCAGCTCTCAGCCACCTTCCCCCAGCTGAAGTCCTTGGAAACCCTC
AATCTGTCCCAGAACAACATCACTGACCTGGGTGCCTACAAACTCGCCGA
GGCCCTGCCTTCGCTCGCTGCATCCCTGCTCAGGCTAAGCTTGTACAATA
ACTGCATCTGCGACGTGGGAGCCGAGAGCTTGGCTCGTGTGCTTCCGGAC
ATGGTGTCCCTCCGGGTGATGGACGTCCAGTACAACAAGTTCACGGCTGC
CGGGGCCCAGCAGCTCGCTGCCAGCCTTCGGAGGTGTCCTCATGTGGAGA
CGCTGGCGATGTGGACGCCCACCATCCCATTCAGTGTCCAGGAACACCTG
CAACAACAGGATTCACGGATCAGCCTGAGATGAGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMRCLAPRPAGSYLSEPQGSS
QCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEEIELYSEPDT
DTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSKDIFK
HIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGS
LLVGPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCL
NLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVPQASQVPPPS
GFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANMTEHKTSPTQ
CPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILVEVDLVQARL
ERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLG
KAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLF
SLGPQPLVAADEVFSHILKRPDRVLLILDAFEELEAQDGFLHSTCGPAPA
EPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKADALFELSGFS
MEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSPTLCRAVCQL
SEALLELGEDAKLPSTLTGLYVGLLGRAALDSPPGALAELAKLAWELGRR
HQSTLQEDQFPSADVRTWAMAKGLVQHPPRAAESELAFPSFLLQCFLGAL
WLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCL
GALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAG
IWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICP
SGLGSLVGLSCVTRFRAALSDTVALWESLRQHGETKLLQAAEEKFTIEPF
KAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLEFALGPVSGP
QAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFPQLKSLETL
NLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLARVLPD
MVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHL
QQQDSRISLR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4774
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H.