cDNA Clone MHC2TA

• Plasmid: pCMV5-HA-1 MHC2TA
• Parent Plasmid: pCMV5
• Expressed: HA-MHC2TA
• Genbank: NM_000246
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCCCGGAATT
CATGCGTTGCCTGGCTCCACGCCCTGCTGGGTCCTACCTGTCAGAGCCCC
AAGGCAGCTCACAGTGTGCCACCATGGAGTTGGGGCCCCTAGAAGGTGGC
TACCTGGAGCTTCTTAACAGCGATGCTGACCCCCTGTGCCTCTACCACTT
CTATGACCAGATGGACCTGGCTGGAGAAGAAGAGATTGAGCTCTACTCAG
AACCCGACACAGACACCATCAACTGCGACCAGTTCAGCAGGCTGTTGTGT
GACATGGAAGGTGATGAAGAGACCAGGGAGGCTTATGCCAATATCGCGGA
ACTGGACCAGTATGTCTTCCAGGACTCCCAGCTGGAGGGCCTGAGCAAGG
ACATTTTCAAGCACATAGGACCAGATGAAGTGATCGGTGAGAGTATGGAG
ATGCCAGCAGAAGTTGGGCAGAAAAGTCAGAAAAGACCCTTCCCAGAGGA
GCTTCCGGCAGACCTGAAGCACTGGAAGCCAGCTGAGCCCCCCACTGTGG
TGACTGGCAGTCTCCTAGTGGGACCAGTGAGCGACTGCTCCACCCTGCCC
TGCCTGCCACTGCCTGCGCTGTTCAACCAGGAGCCAGCCTCCGGCCAGAT
GCGCCTGGAGAAAACCGACCAGATTCCCATGCCTTTCTCCAGTTCCTCGT
TGAGCTGCCTGAATCTCCCTGAGGGACCCATCCAGTTTGTCCCCACCATC
TCCACTCTGCCCCATGGGCTCTGGCAAATCTCTGAGGCTGGAACAGGGGT
CTCCAGTATATTCATCTACCATGGTGAGGTGCCCCAGGCCAGCCAAGTAC
CCCCTCCCAGTGGATTCACTGTCCACGGCCTCCCAACATCTCCAGACCGG
CCAGGCTCCACCAGCCCCTTCGCTCCATCAGCCACTGACCTGCCCAGCAT
GCCTGAACCTGCCCTGACCTCCCGAGCAAACATGACAGAGCACAAGACGT
CCCCCACCCAATGCCCGGCAGCTGGAGAGGTCTCCAACAAGCTTCCAAAA
TGGCCTGAGCCGGTGGAGCAGTTCTACCGCTCACTGCAGGACACGTATGG
TGCCGAGCCCGCAGGCCCGGATGGCATCCTAGTGGAGGTGGATCTGGTGC
AGGCCAGGCTGGAGAGGAGCAGCAGCAAGAGCCTGGAGCGGGAACTGGCC
ACCCCGGACTGGGCAGAACGGCAGCTGGCCCAAGGAGGCCTGGCTGAGGT
GCTGTTGGCTGCCAAGGAGCACCGGCGGCCGCGTGAGACACGAGTGATTG
CTGTGCTGGGCAAAGCTGGTCAGGGCAAGAGCTATTGGGCTGGGGCAGTG
AGCCGGGCCTGGGCTTGTGGCCGGCTTCCCCAGTACGACTTTGTCTTCTC
TGTCCCCTGCCATTGCTTGAACCGTCCGGGGGATGCCTATGGCCTGCAGG
ATCTGCTCTTCTCCCTGGGCCCACAGCCACTCGTGGCGGCCGATGAGGTT
TTCAGCCACATCTTGAAGAGACCTGACCGCGTTCTGCTCATCCTAGACGC
CTTCGAGGAGCTGGAAGCGCAAGATGGCTTCCTGCACAGCACGTGCGGAC
CGGCACCGGCGGAGCCCTGCTCCCTCCGGGGGCTGCTGGCCGGCCTTTTC
CAGAAGAAGCTGCTCCGAGGTTGCACCCTCCTCCTCACAGCCCGGCCCCG
GGGCCGCCTGGTCCAGAGCCTGAGCAAGGCCGACGCCCTATTTGAGCTGT
CCGGCTTCTCCATGGAGCAGGCCCAGGCATACGTGATGCGCTACTTTGAG
AGCTCAGGGATGACAGAGCACCAAGACAGAGCCCTGACGCTCCTCCGGGA
CCGGCCACTTCTTCTCAGTCACAGCCACAGCCCTACTTTGTGCCGGGCAG
TGTGCCAGCTCTCAGAGGCCCTGCTGGAGCTTGGGGAGGACGCCAAGCTG
CCCTCCACGCTCACGGGACTCTATGTCGGCCTGCTGGGCCGTGCAGCCCT
CGACAGCCCCCCCGGGGCCCTGGCAGAGCTGGCCAAGCTGGCCTGGGAGC
TGGGCCGCAGACATCAAAGTACCCTACAGGAGGACCAGTTCCCATCCGCA
GACGTGAGGACCTGGGCGATGGCCAAAGGCTTAGTCCAACACCCACCGCG
GGCCGCAGAGTCCGAGCTGGCCTTCCCCAGCTTCCTCCTGCAATGCTTCC
TGGGGGCCCTGTGGCTGGCTCTGAGTGGCGAAATCAAGGACAAGGAGCTC
CCGCAGTACCTAGCATTGACCCCAAGGAAGAAGAGGCCCTATGACAACTG
GCTGGAGGGCGTGCCACGCTTTCTGGCTGGGCTGATCTTCCAGCCTCCCG
CCCGCTGCCTGGGAGCCCTACTCGGGCCATCGGCGGCTGCCTCGGTGGAC
AGGAAGCAGAAGGTGCTTGCGAGGTACCTGAAGCGGCTGCAGCCGGGGAC
ACTGCGGGCGCGGCAGCTGCTTGAGCTGCTGCACTGCGCCCACGAGGCCG
AGGAGGCTGGAATTTGGCAGCACGTGGTACAGGAGCTCCCCGGCCGCCTC
TCTTTTCTGGGCACCCGCCTCACGCCTCCTGATGCACATGTACTGGGCAA
GGCCTTGGAGGCGGCGGGCCAAGACTTCTCCCTGGACCTCCGCAGCACTG
GCATTTGCCCCTCTGGATTGGGGAGCCTCGTGGGACTCAGCTGTGTCACC
CGTTTCAGGGCTGCCTTGAGCGACACGGTGGCGCTGTGGGAGTCCCTGCG
GCAGCATGGGGAGACCAAGCTACTTCAGGCAGCAGAGGAGAAGTTCACCA
TCGAGCCTTTCAAAGCCAAGTCCCTGAAGGATGTGGAAGACCTGGGAAAG
CTTGTGCAGACTCAGAGGACGAGAAGTTCCTCGGAAGACACAGCTGGGGA
GCTCCCTGCTGTTCGGGACCTAAAGAAACTGGAGTTTGCGCTGGGCCCTG
TCTCAGGCCCCCAGGCTTTCCCCAAACTGGTGCGGATCCTCACGGCCTTT
TCCTCCCTGCAGCATCTGGACCTGGATGCGCTGAGTGAGAACAAGATCGG
GGACGAGGGTGTCTCGCAGCTCTCAGCCACCTTCCCCCAGCTGAAGTCCT
TGGAAACCCTCAATCTGTCCCAGAACAACATCACTGACCTGGGTGCCTAC
AAACTCGCCGAGGCCCTGCCTTCGCTCGCTGCATCCCTGCTCAGGCTAAG
CTTGTACAATAACTGCATCTGCGACGTGGGAGCCGAGAGCTTGGCTCGTG
TGCTTCCGGACATGGTGTCCCTCCGGGTGATGGACGTCCAGTACAACAAG
TTCACGGCTGCCGGGGCCCAGCAGCTCGCTGCCAGCCTTCGGAGGTGTCC
TCATGTGGAGACGCTGGCGATGTGGACGCCCACCATCCCATTCAGTGTCC
AGGAACACCTGCAACAACAGGATTCACGGATCAGCCTGAGATGAGTCGAC

Amino Acid Sequence

MYPYDVPDYAGSPEFMRCLAPRPAGSYLSEPQGSSQCATMELGPLEGGYL
ELLNSDADPLCLYHFYDQMDLAGEEEIELYSEPDTDTINCDQFSRLLCDM
EGDEETREAYANIAELDQYVFQDSQLEGLSKDIFKHIGPDEVIGESMEMP
AEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGSLLVGPVSDCSTLPCL
PLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCLNLPEGPIQFVPTIST
LPHGLWQISEAGTGVSSIFIYHGEVPQASQVPPPSGFTVHGLPTSPDRPG
STSPFAPSATDLPSMPEPALTSRANMTEHKTSPTQCPAAGEVSNKLPKWP
EPVEQFYRSLQDTYGAEPAGPDGILVEVDLVQARLERSSSKSLERELATP
DWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLGKAGQGKSYWAGAVSR
AWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLFSLGPQPLVAADEVFS
HILKRPDRVLLILDAFEELEAQDGFLHSTCGPAPAEPCSLRGLLAGLFQK
KLLRGCTLLLTARPRGRLVQSLSKADALFELSGFSMEQAQAYVMRYFESS
GMTEHQDRALTLLRDRPLLLSHSHSPTLCRAVCQLSEALLELGEDAKLPS
TLTGLYVGLLGRAALDSPPGALAELAKLAWELGRRHQSTLQEDQFPSADV
RTWAMAKGLVQHPPRAAESELAFPSFLLQCFLGALWLALSGEIKDKELPQ
YLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCLGALLGPSAAASVDRK
QKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAGIWQHVVQELPGRLSF
LGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICPSGLGSLVGLSCVTRF
RAALSDTVALWESLRQHGETKLLQAAEEKFTIEPFKAKSLKDVEDLGKLV
QTQRTRSSSEDTAGELPAVRDLKKLEFALGPVSGPQAFPKLVRILTAFSS
LQHLDLDALSENKIGDEGVSQLSATFPQLKSLETLNLSQNNITDLGAYKL
AEALPSLAASLLRLSLYNNCICDVGAESLARVLPDMVSLRVMDVQYNKFT
AAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHLQQQDSRISLR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4773
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA.