cDNA Clone MHC2TA

• Plasmid: pGEX6P-1-MHC2TA
• Parent Plasmid: pGEX6P1
• Expressed: GST-MHC2TA
• Genbank: NM_000246
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCATGCGTTGCCTGGCTCCACGCCCTGCTGGGTCCTACCTGTCAGAGCCC
CAAGGCAGCTCACAGTGTGCCACCATGGAGTTGGGGCCCCTAGAAGGTGG
CTACCTGGAGCTTCTTAACAGCGATGCTGACCCCCTGTGCCTCTACCACT
TCTATGACCAGATGGACCTGGCTGGAGAAGAAGAGATTGAGCTCTACTCA
GAACCCGACACAGACACCATCAACTGCGACCAGTTCAGCAGGCTGTTGTG
TGACATGGAAGGTGATGAAGAGACCAGGGAGGCTTATGCCAATATCGCGG
AACTGGACCAGTATGTCTTCCAGGACTCCCAGCTGGAGGGCCTGAGCAAG
GACATTTTCAAGCACATAGGACCAGATGAAGTGATCGGTGAGAGTATGGA
GATGCCAGCAGAAGTTGGGCAGAAAAGTCAGAAAAGACCCTTCCCAGAGG
AGCTTCCGGCAGACCTGAAGCACTGGAAGCCAGCTGAGCCCCCCACTGTG
GTGACTGGCAGTCTCCTAGTGGGACCAGTGAGCGACTGCTCCACCCTGCC
CTGCCTGCCACTGCCTGCGCTGTTCAACCAGGAGCCAGCCTCCGGCCAGA
TGCGCCTGGAGAAAACCGACCAGATTCCCATGCCTTTCTCCAGTTCCTCG
TTGAGCTGCCTGAATCTCCCTGAGGGACCCATCCAGTTTGTCCCCACCAT
CTCCACTCTGCCCCATGGGCTCTGGCAAATCTCTGAGGCTGGAACAGGGG
TCTCCAGTATATTCATCTACCATGGTGAGGTGCCCCAGGCCAGCCAAGTA
CCCCCTCCCAGTGGATTCACTGTCCACGGCCTCCCAACATCTCCAGACCG
GCCAGGCTCCACCAGCCCCTTCGCTCCATCAGCCACTGACCTGCCCAGCA
TGCCTGAACCTGCCCTGACCTCCCGAGCAAACATGACAGAGCACAAGACG
TCCCCCACCCAATGCCCGGCAGCTGGAGAGGTCTCCAACAAGCTTCCAAA
ATGGCCTGAGCCGGTGGAGCAGTTCTACCGCTCACTGCAGGACACGTATG
GTGCCGAGCCCGCAGGCCCGGATGGCATCCTAGTGGAGGTGGATCTGGTG
CAGGCCAGGCTGGAGAGGAGCAGCAGCAAGAGCCTGGAGCGGGAACTGGC
CACCCCGGACTGGGCAGAACGGCAGCTGGCCCAAGGAGGCCTGGCTGAGG
TGCTGTTGGCTGCCAAGGAGCACCGGCGGCCGCGTGAGACACGAGTGATT
GCTGTGCTGGGCAAAGCTGGTCAGGGCAAGAGCTATTGGGCTGGGGCAGT
GAGCCGGGCCTGGGCTTGTGGCCGGCTTCCCCAGTACGACTTTGTCTTCT
CTGTCCCCTGCCATTGCTTGAACCGTCCGGGGGATGCCTATGGCCTGCAG
GATCTGCTCTTCTCCCTGGGCCCACAGCCACTCGTGGCGGCCGATGAGGT
TTTCAGCCACATCTTGAAGAGACCTGACCGCGTTCTGCTCATCCTAGACG
CCTTCGAGGAGCTGGAAGCGCAAGATGGCTTCCTGCACAGCACGTGCGGA
CCGGCACCGGCGGAGCCCTGCTCCCTCCGGGGGCTGCTGGCCGGCCTTTT
CCAGAAGAAGCTGCTCCGAGGTTGCACCCTCCTCCTCACAGCCCGGCCCC
GGGGCCGCCTGGTCCAGAGCCTGAGCAAGGCCGACGCCCTATTTGAGCTG
TCCGGCTTCTCCATGGAGCAGGCCCAGGCATACGTGATGCGCTACTTTGA
GAGCTCAGGGATGACAGAGCACCAAGACAGAGCCCTGACGCTCCTCCGGG
ACCGGCCACTTCTTCTCAGTCACAGCCACAGCCCTACTTTGTGCCGGGCA
GTGTGCCAGCTCTCAGAGGCCCTGCTGGAGCTTGGGGAGGACGCCAAGCT
GCCCTCCACGCTCACGGGACTCTATGTCGGCCTGCTGGGCCGTGCAGCCC
TCGACAGCCCCCCCGGGGCCCTGGCAGAGCTGGCCAAGCTGGCCTGGGAG
CTGGGCCGCAGACATCAAAGTACCCTACAGGAGGACCAGTTCCCATCCGC
AGACGTGAGGACCTGGGCGATGGCCAAAGGCTTAGTCCAACACCCACCGC
GGGCCGCAGAGTCCGAGCTGGCCTTCCCCAGCTTCCTCCTGCAATGCTTC
CTGGGGGCCCTGTGGCTGGCTCTGAGTGGCGAAATCAAGGACAAGGAGCT
CCCGCAGTACCTAGCATTGACCCCAAGGAAGAAGAGGCCCTATGACAACT
GGCTGGAGGGCGTGCCACGCTTTCTGGCTGGGCTGATCTTCCAGCCTCCC
GCCCGCTGCCTGGGAGCCCTACTCGGGCCATCGGCGGCTGCCTCGGTGGA
CAGGAAGCAGAAGGTGCTTGCGAGGTACCTGAAGCGGCTGCAGCCGGGGA
CACTGCGGGCGCGGCAGCTGCTTGAGCTGCTGCACTGCGCCCACGAGGCC
GAGGAGGCTGGAATTTGGCAGCACGTGGTACAGGAGCTCCCCGGCCGCCT
CTCTTTTCTGGGCACCCGCCTCACGCCTCCTGATGCACATGTACTGGGCA
AGGCCTTGGAGGCGGCGGGCCAAGACTTCTCCCTGGACCTCCGCAGCACT
GGCATTTGCCCCTCTGGATTGGGGAGCCTCGTGGGACTCAGCTGTGTCAC
CCGTTTCAGGGCTGCCTTGAGCGACACGGTGGCGCTGTGGGAGTCCCTGC
GGCAGCATGGGGAGACCAAGCTACTTCAGGCAGCAGAGGAGAAGTTCACC
ATCGAGCCTTTCAAAGCCAAGTCCCTGAAGGATGTGGAAGACCTGGGAAA
GCTTGTGCAGACTCAGAGGACGAGAAGTTCCTCGGAAGACACAGCTGGGG
AGCTCCCTGCTGTTCGGGACCTAAAGAAACTGGAGTTTGCGCTGGGCCCT
GTCTCAGGCCCCCAGGCTTTCCCCAAACTGGTGCGGATCCTCACGGCCTT
TTCCTCCCTGCAGCATCTGGACCTGGATGCGCTGAGTGAGAACAAGATCG
GGGACGAGGGTGTCTCGCAGCTCTCAGCCACCTTCCCCCAGCTGAAGTCC
TTGGAAACCCTCAATCTGTCCCAGAACAACATCACTGACCTGGGTGCCTA
CAAACTCGCCGAGGCCCTGCCTTCGCTCGCTGCATCCCTGCTCAGGCTAA
GCTTGTACAATAACTGCATCTGCGACGTGGGAGCCGAGAGCTTGGCTCGT
GTGCTTCCGGACATGGTGTCCCTCCGGGTGATGGACGTCCAGTACAACAA
GTTCACGGCTGCCGGGGCCCAGCAGCTCGCTGCCAGCCTTCGGAGGTGTC
CTCATGTGGAGACGCTGGCGATGTGGACGCCCACCATCCCATTCAGTGTC
CAGGAACACCTGCAACAACAGGATTCACGGATCAGCCTGAGATGAGTCGA
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMRCLAPRPAGSYLSEP
QGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEEIELYS
EPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSK
DIFKHIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTV
VTGSLLVGPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSS
LSCLNLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVPQASQV
PPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANMTEHKT
SPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILVEVDLV
QARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVI
AVLGKAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQ
DLLFSLGPQPLVAADEVFSHILKRPDRVLLILDAFEELEAQDGFLHSTCG
PAPAEPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKADALFEL
SGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSPTLCRA
VCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPPGALAELAKLAWE
LGRRHQSTLQEDQFPSADVRTWAMAKGLVQHPPRAAESELAFPSFLLQCF
LGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPP
ARCLGALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEA
EEAGIWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRST
GICPSGLGSLVGLSCVTRFRAALSDTVALWESLRQHGETKLLQAAEEKFT
IEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLEFALGP
VSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFPQLKS
LETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLAR
VLPDMVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSV
QEHLQQQDSRISLR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4772
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.