cDNA Clone MHC2TA

• Plasmid: pEBG6P-1 MHC2TA with card domain
• Parent Plasmid: pEBG6P
• Expressed: GST-MHC2TA with card domain
• Genbank: BAE25629
• Tag: GST
• Species: Mouse

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGAACCACTTCCAGGCCATCCTGGCCCAAGTACAGACA
CTGCTCTCCAGCCAGAAGCCCAGGCAGGTGCGGGCCCTCCTGGATGGCCT
GCTGGAAGAAGAGCTGCTCTCACGGGAATACCACTGTGCCTTGCTGCATG
AGCCTGATGGTGATGCCCTGGCCCGGAAGATTTCCCTGACCCTGCTGGAG
AAAGGGGACTTAGACTTGACTTTCTTGAGCTGGGTCTGCAACAGTCTGCA
GGCTCCCACGGTAGAGAGGGGCACCAGCTACAGGGACCATGGAGACCATA
GTCTGTGTGCCACCATGGATCTGGGATCTCCAGAGGGCAGCTACCTGGAA
CTCCTTAACAGTGATGCCGACCCCCTACATCTCTACCACCTCTATGACCA
GATGGACCTGGCTGGGGAGGAGGAGATCGAACTCAGCTCAGAGCCAGACA
CAGATACCATCAACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAA
CTGGATGAAGAGACCCGGGAGGCCTATGCCAACATTGCGGAACTGGATCA
GTACGTGTTCCAGGATACCCAGCTCGAGGGCCTGAGCAAGGACCTCTTCA
TAGAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATAGAGATCCCT
GTAGAAGCAGGACAGAAGCCTCAGAAGAGACGCTTCCCGGAAGAGCATGC
TATGGACTCAAAGCACAGGAAGCTAGTGCCCACCTCTAGGACCTCACTGA
ACTATTTGGATCTCCCCACTGGGCACATCCAGATCTTCACCACTCTGCCC
CAGGGACTCTGGCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCT
AATCTACCACGGTGAGATGCCCCAGGTCAACCAAGTGCTCCCTTCAAGCA
GCCTCAGTATCCCCAGTCTCCCCGAGTCCCCAGACCGGCCTGGCTCCACC
AGCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCCCGAACCTGC
GCTGACCTCCCGTGTAAATGAGACAGAGGACACATCTCCCTCCCCATGCC
AAGAGGGTCCCGAGTCTTCCATCAAGCTTCCAAAATGGCCAGAGGCTGTG
GAGCGATTCCAGCACTCCCTACAGGACAAATACAAGGCATTGCCCCAGAG
CCCAAGGGGTCCTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAA
GAGGCAGCAACAAGAGCCAGGAAAGGGAGCTGGCCACTCCCGACTGGACA
GAGCGCCAGCTAGCCCACGGTGGTCTGGCAGAGGTACTTCAGGTTGTCAG
TGACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGCTGGGCAAGG
CTGGCCAGGGAAAGAGCCACTGGGCCAGGACAGTGAGTCACACCTGGGCA
TGTGGCCAGTTGCTACAATATGACTTTGTCTTCTATGTCCCCTGTCATTG
CTTGGATCGTCCCGGGGACACCTACCACCTGCGGGATCTGCTCTGTCCCC
CGAGCCTGCAGCCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTG
AGGCAGCCAGACCGTGTTCTGCTCATCCTAGATGCTTTCGAGGAGCTAGA
GGCCCAAGATGGCCTCCTGCACGGACCCTGTGGATCTCTGTCCCCAGAGC
CCTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGGAAGCTACTG
CGAGGCTGCACACTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCA
GAGCCTGAGCAAGGCAGATGCCATCTTTGAGGTGCCCAGCTTCTCTACCA
AGCAGGCCAAGACTTACATGAGGCACTACTTTGAGAACTCAGGGACAGCG
GGGAACCAAGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTG
CAGCTATAGTCACAGCCCTGTTGTGTGCAGGGCTGTGTGCCAGCTCTCCA
AGGCCCTGCTAGAACAGGGCACAGAGGCCCAGCTACCTTGTACACTTACA
GGACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAGTCCTCCCGG
AGCCTTAGTCGAGCTGGCCAAGCTGGCCTGGGAGCTGGGACGAAGACACC
AAAGCACCTTGCAAGAAACCCGGTTTTCATCCGTGGAGGTGAAAACCTGG
GCAGTGACCCAAGGCTTGATGCAGCAGACCCTGGAGACCACGGAGGCTCA
ACTGGCCTTCTCCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGC
TGGCACAGTGCAATGAAATCAAAGACAAGGAGCTGCCACAGTACCTGGCC
TTGACTCCGAGGAAGAAGAGACCCTATGACAACTGGCTGGAGGGTGTACC
ACGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACTGCCTGGGAG
CTCTGGTGGAGCCTGCAGTGGCTGCAGTGGCGGATAGGAAACAGAAGGTT
CTTACCAGGTACCTGAAGCGCCTGAAGCTGGGGACACTCCGGGCAGGGAG
GCTGCTGGAGCTGCTCCACTGTGCCCACGAGACACAGCAACCTGGGATAT
GGGAGCATGTTGCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACC
CGGCTCACACCCCCAGATGTGTATGTGCTGGGCAGGGCCTTGGAGACAGC
CAGCCAGGACTTCTCCTTGGACCTTCGTCAGACTGGCGTTGAGCCTTCTG
GACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGGGCCTCC
TTGAGTGATACAATGGCATTATGGGAGTCCCTTCAGCAGCAGGGAGAAGC
CCAGCTACTCCAGGCGGCAGAGGAGAAGTTCACCATTGAGCCATTTAAAG
CCAAATCCCCAAAGGATGTGGAAGACCTGGATCGTCTCGTGCAGACCCAG
AGGCTGAGAAACCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCG
GGACCTTAAGAAGCTAGAGTTTGCGTTGGGCCCCATCTTGGGCCCCCAGG
CTTTCCCCACACTGGCAAAGATCCTTCCAGCCTTCTCTTCTCTGCAACAC
CTGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAAGGGTGTGTC
GGAGCTCTCAGCCACCTTCCCTCAGCTGAAGGCCCTGGAGACGCTCAACT
TGTCCCAAAACAACATCACTGATGTGGGTGCCTGCAAGCTTGCAGAAGCT
CTGCCAGCCCTAGCCAAGTCCCTCCTAAGGCTGAGCTTGTACAATAACTG
CATCTGTGACAAAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGG
TGTCCCTGCGTGTGATGGATGTCCAGTTCAACAAGTTCACGGCTGCCGGT
GCCCAGCAACTGGCCTCCAGCCTTCAGAAGTGCCCTCAGGTGGAAACACT
GGCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAACACCTGCAGC
AGCTGGATGCCAGGATCAGTCTGAGATGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNHFQAILAQVQT
LLSSQKPRQVRALLDGLLEEELLSREYHCALLHEPDGDALARKISLTLLE
KGDLDLTFLSWVCNSLQAPTVERGTSYRDHGDHSLCATMDLGSPEGSYLE
LLNSDADPLHLYHLYDQMDLAGEEEIELSSEPDTDTINCDQFSKLLQDME
LDEETREAYANIAELDQYVFQDTQLEGLSKDLFIEHIGAEEGFGENIEIP
VEAGQKPQKRRFPEEHAMDSKHRKLVPTSRTSLNYLDLPTGHIQIFTTLP
QGLWQISGAGTGLSSVLIYHGEMPQVNQVLPSSSLSIPSLPESPDRPGST
SPFTPSAADLPSMPEPALTSRVNETEDTSPSPCQEGPESSIKLPKWPEAV
ERFQHSLQDKYKALPQSPRGPLVAVELVRARLERGSNKSQERELATPDWT
ERQLAHGGLAEVLQVVSDCRRPGETQVVAVLGKAGQGKSHWARTVSHTWA
CGQLLQYDFVFYVPCHCLDRPGDTYHLRDLLCPPSLQPLAMDDEVLDYIV
RQPDRVLLILDAFEELEAQDGLLHGPCGSLSPEPCSLRGLLAGIFQRKLL
RGCTLLLTARPRGRLAQSLSKADAIFEVPSFSTKQAKTYMRHYFENSGTA
GNQDKALGLLEGQPLLCSYSHSPVVCRAVCQLSKALLEQGTEAQLPCTLT
GLYVSLLGPAAQNSPPGALVELAKLAWELGRRHQSTLQETRFSSVEVKTW
AVTQGLMQQTLETTEAQLAFSSFLLQCFLGAVWLAQCNEIKDKELPQYLA
LTPRKKRPYDNWLEGVPRFLAGLVFQPRAHCLGALVEPAVAAVADRKQKV
LTRYLKRLKLGTLRAGRLLELLHCAHETQQPGIWEHVAHQLPGHLSFLGT
RLTPPDVYVLGRALETASQDFSLDLRQTGVEPSGLGNLVGLSCVTSFRAS
LSDTMALWESLQQQGEAQLLQAAEEKFTIEPFKAKSPKDVEDLDRLVQTQ
RLRNPSEDAAKDLPAIRDLKKLEFALGPILGPQAFPTLAKILPAFSSLQH
LDLDSLSENKIGDKGVSELSATFPQLKALETLNLSQNNITDVGACKLAEA
LPALAKSLLRLSLYNNCICDKGAKSLAQVLPDMVSLRVMDVQFNKFTAAG
AQQLASSLQKCPQVETLAMWTPTIPFGVQEHLQQLDARISLR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4771
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.