cDNA Clone NEDD4-2 (rat)

• Plasmid: pEBG6P-1 NEDD4-2 S327A S328D
• Parent Plasmid: pEBG6P
• Expressed: GST-NEDD4-2b1 S327A S328D
• Genbank: NP_056092
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCATCGATGCGGCCGCCAGTGTGATGGAT
ATCTGCAGAATTCGCCCTTCGGCCGCGGAGCGACCCTATACATTTAAGGA
CTTTCTCCTCAGACCAAGAAGTCATAAGTCTCGAGTTAAGGGATTTTTGC
GATTGAAAATGGCCCATATGCCAAAAAATGGAGGTCAAGATGAAGAAAAC
AGTGACCAGAGGGATGACATGGAGCATGGATGGGAAGTTGTTGACTCAAA
TGACTCGGCTTCTCAGCACCAAGAGGAACTTCCTCCTCCTCCTCTGCCTC
CCGGGTGGGAAGAAAAAGTGAACAATTTAGGCCGAACTTACTATGTCAAC
CACAACAACCGGACCACTCAGTGGCACAGACCAAGCCTGATGGACGTGTC
CTCGGAGTCGGACAATAACATCAGACAGATCAACCAGGAGGCAGCACACC
GGCGCTTCCGCTCCCGCAGGCACATCAGCGAAGACTTGGAGCCCGAGCCC
TCGGAGGGCGGGGATGTCCCCGAGCCTTGGGAGACCATTTCAGAGGAAGT
GAATATCGCTGGAGACTCTCTCGGTCTGGCTCTGCCCCCACCGCCGGCCT
CCCCAGGATCTCGGACCAGCCCTCAGGAGCTGTCAGAGGAACTATGCAGA
AGGCTTCAGATCACTCCAGACTCCAATGGGGAACAGTTCAGCTCTTTGAT
TCAAAGAGAACCCTCCTCAAGGTTGAGGTCATGCAGTGTCACCGACGCAG
TTGCAGAACAGGGCCATCTACCACCGCCCAGTGCCCCAGCTGGGAGAGCG
CGTTCATCAACTGTCACGGGTGGTGAGGAACCAACGCCATCAGTGGCCTA
TGTACATACCACGCCGGGTCTGCCCTCAGGCTGGGAAGAAAGAAAAGATG
CTAAGGGGCGCACATACTATGTCAATCATAACAATCGAACCACAACTTGG
ACTCGACCTATCATGCAGCTTGCAGAAGATGGTGCGTCCGGATCAGCCAC
AAACAGTAACAACCATCTAATCGAGCCTCAGATCCGCCGGCCTCGTAGCC
TCGCGGACCCAACAGTAACTTTATCTGCCCCGCTGGAGGGTGCCAAGGAC
TCACCCGTACGTCGGGCTGTGAAAGACACCCTTTCCAACCCACAGTCCCC
ACAGCCATCACCTTACAACTCCCCCAAACCACAACACAAAGTCACACAGA
GCTTCTTGCCGCCCGGCTGGGAAATGAGGATAGCGCCAAACGGCCGGCCC
TTCTTCATTGATCATAACACAAAGACTACAACCTGGGAAGATCCACGTTT
GAAGTTTCCAGTACATATGCGGTCAAAGACATCTTTAAACCCCAATGACC
TTGGCCCCCTTCCTCCTGGCTGGGAAGAAAGAATTCACTTGGATGGCCGA
ACGTTTTATATTGATCATAATAGCAAAATTACTCAGTGGGAAGACCCAAG
ACTGCAGAACCCAGCTATTACTGGTCCGGCTGTCCCTTACTCCAGAGAAT
TTAAGCAGAAATATGACTACTTCAGGAAGAAATTAAAGAAACCTGCTGAT
ATCCCTAATAGGTTTGAAATGAAACTTCACAGAGATAACATATTTGAAGA
GTCCTATCGGAGAATTATGTCCGTGAAAAGACCAGATGTCCTAAAAGCTA
GACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTATGGGGGTGTG
GCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCTACTA
CGGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATCAACC
CTAATTCAGGCCTCTGTAATGAGGATCATTTGTCCTACTTCACTTTTATT
GGAAGAGTTGCTGGTCTGGCCGTATTTCATGGGAAGCTCTTAGATGGTTT
CTTCATTAGACCATTTTACAAGATGATGTTGGGAAAGCAGATAACCCTGA
ATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTGAAATGGATC
CTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACGAAGA
AAACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCAGAAA
TAATGGTCACAAATGAAAACAAAAGGGAATATATCGACTTAGTCATCCAG
TGGAGATTTGTGAACAGGGTCCAGAAGCAGATGAACGCCTTCTTGGAGGG
ATTCACAGAACTACTTCCTATTGATTTGATTAAAATTTTTGACGAAAATG
AGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTGAATGACTGG
AGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCGTCAT
TCAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGTATCC
GGTTACTGCAGTTTGTCACAGGGACATCGCGAGTACCTATGAATGGATTT
GCCGAACTTTATGGTTCCAATGGTCCTCAGCTGTTTACAATAGAGCAATG
GGGCAGTCCTGAGAAACTGCCCAGAGCTCACACATGCTTTAATCGCCTTG
ACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAACTTCTCATG
GCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAASVMD
ICRIRPSAAERPYTFKDFLLRPRSHKSRVKGFLRLKMAHMPKNGGQDEEN
SDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVNNLGRTYYVN
HNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEP
SEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELCR
RLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRA
RSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTW
TRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLADPTVTLSAPLEGAKD
SPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRP
FFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGR
TFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPAD
IPNRFEMKLHRDNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGV
AREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFI
GRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWI
LENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQ
WRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDW
RQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGF
AELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLM
AVENAQGFEGVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4738
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST; This protein differs from NP_056092. The differences are Y11H, D83N, S191C and N504D.