cDNA Clone DAZAP1-b

• Plasmid: pAS2.1 DAZAP1-b 155-407 T315A
• Parent Plasmid: pAS2.1
• Expressed: GAL4-DNA-BD-DAZAP1-b 155-407 T315A
• Genbank: NM_018959
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAA
AAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGA
ACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACT
AGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCT
ATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGG
ATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGA
TATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGG
AAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGTATTG
CAATACCCAGCTTTGACTCATATGGGTTTTGGATTTATTACTTTCGAGGA
CGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGG
GCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAA
GCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAA
CGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGAT
ATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTAT
GGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCAC
CTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCT
TCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCT
CCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACC
AGCCGCTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGG
CTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTAT
GGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTT
CTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAG
GGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAAC
CACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGTCGAC

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQ
APGQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGY
GPPPAGRGAPPPPPPFTSYIVSTPPGGFPPPQGFPQGYGAPPQFSFGYGP
PPPPPDQFAPPGVPPPPAAPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPY
GQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQN
HNVQGFHPYRR*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4733
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression vector with GAL4 DNA-BD fused to Dazap1-b.