cDNA Clone DAZAP1-b

• Plasmid: pAS2.1 DAZAP1-b T315A
• Parent Plasmid: pAS2.1
• Expressed: GAL4-DNA-BD-DAZAP1-b T315A
• Genbank: NM_018959
• Tag: GAL4 DNA-BD

Sequence of Insert

ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAA
AAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGA
ACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACT
AGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCT
ATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGG
ATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGA
TATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGG
AAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGTATTG
CAATACCCAGCTTTGACTCATATGAACAACTCGGGCGCCGACGAGATCGG
GAAGCTCTTCGTGGGCGGTCTTGACTGGAGCACGACCCAAGAGACTCTGC
GCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTTATCATGAAA
GATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGA
CCCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATG
GCCGAAACATCGACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAG
AGAACACGGCCGAAGGAAGGATGGCAGAAAGGACCCAGGAGCGATAACAG
TAAATCAAATAAGATATTTGTCGGTGGAATTCCTCACAATTGTGGTGAGA
CAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACGGAGGTAGTC
ATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTAC
TTTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACG
ACATCATGGGCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGC
AAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGT
TGTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGC
AGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATT
GGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCC
ACCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTC
CCCAGGGCTTCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGC
TACGGGCCTCCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCC
TCCTCCACCAGCCGCTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTC
CGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGAC
TTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGG
ACAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCT
CCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGA
GGGCAGAACCACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGTCGA
C

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMNNSGADEIGKLFVGGLDWSTTQETLRSYFSQYGEVVDCVIMK
DKTTNQSRGFGFVKFKDPNCVGTVLASRPHTLDGRNIDPKPCTPRGMQPE
RTRPKEGWQKGPRSDNSKSNKIFVGGIPHNCGETELREYFKKFGVVTEVV
MIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDS
KSQAPGQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAGQAI
GGYGPPPAGRGAPPPPPPFTSYIVSTPPGGFPPPQGFPQGYGAPPQFSFG
YGPPPPPPDQFAPPGVPPPPAAPGAAPLAFPPPPSQAAPDMSKPPTAQPD
FPYGQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGR
GQNHNVQGFHPYRR*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4731
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression vector with GAL4 DNA-BD fused to Dazap1-b.