cDNA Clone IRAK1

• Plasmid: pEBG6P-1 IRAK1 K772R
• Parent Plasmid: pEBG6P
• Expressed: GST-IRAK1 K772R
• Genbank: AAC41949
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCATCGATGCGGCCGCGATGGCCGGGGGG
CCGGGCCCGGGGGAGCCCGCAGCCCCCGGCGCCCAGCACTTCTTGTACGA
GGTGCCGCCCTGGGTCATGTGCCGCTTCTACAAAGTGATGGACGCCCTGG
AGCCCGCCGACTGGTGCCAGTTCGCCGCCCTGATCGTGCGCGACCAGACC
GAGCTGCGGCTGTGCGAGCGCTCCGGGCAGCGCACGGCCAGCGTCCTGTG
GCCCTGGATCAACCGCAACGCCCGTGTGGCCGACCTCGTGCACATCCTCA
CGCACCTGCAGCTGCTCCGTGCGCGGGACATCATCACAGCCTGGCACCCT
CCCGCCCCGCTTCCGTCCCCAGGCACCACTGCCCCGAGGCCCAGCAGCAT
CCCTGCACCCGCCGAGGCCGAGGCCTGGAGCCCCCGGAAGTTGCCATCCT
CAGCCTCCACCTTCCTCTCCCCAGCTTTTCCAGGCTCCCAGACCCATTCA
GGGCCTGAGCTCGGCCTGGTCCCAAGCCCTGCTTCCCTGTGGCCTCCACC
GCCATCTCCAGCCCCTTCTTCTACCAAGCCAGGCCCAGAGAGCTCAGTGT
CCCTCCTGCAGGGAGCCCGCCCCTCTCCGTTTTGCTGGCCCCTCTGTGAG
ATTTCCCGGGGCACCCACAACTTCTCGGAGGAGCTCAAGATCGGGGAGGG
TGGCTTTGGGTGCGTGTACCGGGCGGTGATGAGGAACACGGTGTATGCTG
TGAAGAGGCTGAAGGAGAACGCTGACCTGGAGTGGACTGCAGTGAAGCAG
AGCTTCCTGACCGAGGTGGAGCAGCTGTCCAGGTTTCGTCACCCAAACAT
TGTGGACTTTGCTGGCTACTGTGCTCAGAACGGCTTCTACTGCCTGGTGT
ACGGCTTCCTGCCCAACGGCTCCCTGGAGGACCGTCTCCACTGCCAGACC
CAGGCCTGCCCACCTCTCTCCTGGCCTCAGCGACTGGACATCCTTCTGGG
TACAGCCCGGGCAATTCAGTTTCTACATCAGGACAGCCCCAGCCTCATCC
ATGGAGACATCAAGAGTTCCAACGTCCTTCTGGATGAGAGGCTGACACCC
AAGCTGGGAGACTTTGGCCTGGCCCGGTTCAGCCGCTTTGCCGGGTCCAG
CCCCAGCCAGAGCAGCATGGTGGCCCGGACACAGACAGTGCGGGGCACCC
TGGCCTACCTGCCCGAGGAGTACATCAAGACGGGAAGGCTGGCTGTGGAC
ACGGACACCTTCAGCTTTGGGGTGGTAGTGCTAGAGACCTTGGCTGGTCA
GAGGGCTGTGAAGACGCACGGTGCCAGGACCAAGTATCTGAAAGACCTGG
TGGAAGAGGAGGCTGAGGAGGCTGGAGTGGCTTTGAGAAGCACCCAGAGC
ACACTGCAAGCAGGTCTGGCTGCAGATGCCTGGGCTGCTCCCATCGCCAT
GCAGATCTACAAGAAGCACCTGGACCCCAGGCCCGGGCCCTGCCCACCTG
AGCTGGGCCTGGGCCTGGGCCAGCTGGCCTGCTGCTGCCTGCACCGCCGG
GCCAAAAGGAGGCCTCCTATGACCCAGGTGTACGAGAGGCTAGAGAAGCT
GCAGGCAGTGGTGGCGGGGGTGCCCGGGCATTTGGAGGCCGCCAGCTGCA
TCCCCCCTTCCCCGCAGGAGAACTCCTACGTGTCCAGCACTGGCAGAGCC
CACAGTGGGGCTGCTCCATGGCAGCCCCTGGCAGCGCCATCAGGAGCCAG
TGCCCAGGCAGCAGAGCAGCTGCAGAGAGGCCCCAACCAGCCCGTGGAGA
GTGACGAGAGCCTAGGCGGCCTCTCTGCTGCCCTGCGCTCCTGGCACTTG
ACTCCAAGCTGCCCTCTGGACCCAGCACCCCTCAGGGAGGCCGGCTGTCC
TCAGGGGGACACGGCAGGAGAATCGAGCTGGGGGAGTGGCCCAGGATCCC
GGCCCACAGCCGTGGAAGGACTGGCCCTTGGCAGCTCTGCATCATCGTCG
TCAGAGCCACCGCAGATTATCATCAACCCTGCCCGACAGAAGATGGTCCA
GCGGCTGGCCCTGTACGAGGATGGGGCCCTGGACAGCCTGCAGCTGCTGT
CGTCCAGCTCCCTCCCAGGCTTGGGCCTGGAACAGGACAGGCAGGGGCCC
GAAGAAAGTGATGAATTTCAGAGCTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAGG
PGPGEPAAPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQT
ELRLCERSGQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWHP
PAPLPSPGTTAPRPSSIPAPAEAEAWSPRKLPSSASTFLSPAFPGSQTHS
GPELGLVPSPASLWPPPPSPAPSSTKPGPESSVSLLQGARPSPFCWPLCE
ISRGTHNFSEELKIGEGGFGCVYRAVMRNTVYAVKRLKENADLEWTAVKQ
SFLTEVEQLSRFRHPNIVDFAGYCAQNGFYCLVYGFLPNGSLEDRLHCQT
QACPPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLTP
KLGDFGLARFSRFAGSSPSQSSMVARTQTVRGTLAYLPEEYIKTGRLAVD
TDTFSFGVVVLETLAGQRAVKTHGARTKYLKDLVEEEAEEAGVALRSTQS
TLQAGLAADAWAAPIAMQIYKKHLDPRPGPCPPELGLGLGQLACCCLHRR
AKRRPPMTQVYERLEKLQAVVAGVPGHLEAASCIPPSPQENSYVSSTGRA
HSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHL
TPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSS
SEPPQIIINPARQKMVQRLALYEDGALDSLQLLSSSSLPGLGLEQDRQGP
EESDEFQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4725
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.