cDNA Clone IRAK3

• Plasmid: pGEX6P-1-IRAK3
• Parent Plasmid: pGEX6P1
• Expressed: GST-IRAK3
• Genbank: BC069388
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGGCGG
GGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGAC
CTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGA
CGGCGCGCTGGGTTGGCGCGGCCTGGCAGAGAGACTTTCAAGCAGCTGGC
TGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGGTAAAAGTGGAACA
AGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGACCATCGGTGACCT
TTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCATTTAATTA
CAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGT
GGATTTCCAAATATATTATTCAAGGAAACAGCCAATGTCACCGTGGATAA
TGTTCTTATTCCTGAACATAATGAAAAAGGAGTACTGCTTAAATCTTCCA
TCAGCTTTCAAAATATCATAGAAGGAACTAGAAATTTCCACAAAGACTTC
CTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGATTCAAAA
CCTAACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTA
AGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTT
CATCACCCAAACATACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAA
GTTCTGTCTGATTTATCCATACATGAGAAATGGAACACTTTTTGACAGAT
TGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCACATTCGAATCGGT
ATATTAATAGGAATATCCAAAGCCATTCACTACCTGCACAACGTTCAACC
ATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATG
ATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCC
CACCTAGAACATCAGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAA
ACATCTGTGGTACATGCCAGAAGAGTACATCAGACAGGGGAAACTTTCCA
TTAAAACAGATGTCTACAGCTTTGGAATTGTAATAATGGAAGTTCTAACA
GGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGCGGGATCT
CCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTC
TAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTC
TGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAAT
GGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCCAGCTTGTATTTTG
CTGAAGATCCTCCCACATCACTAAAGTCCTTCAGGTGTCCTTCTCCTCTA
TTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAAGCCAGAA
TAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTC
AGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTG
GACAAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAG
TTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATAGTTCCATCCCAGG
ACTTAAGGCCCTATAAGGTAAATATAGATCCTTCTTCAGAAGCTCCAGGG
CATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCAAATTTTC
CTGGGATGAATATGAACAGTACAAAAAAGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGNCGARGALSAHTLLFD
LPPALLGELCAVLDSCDGALGWRGLAERLSSSWLDVRHIEKYVDQGKSGT
RELLWSWAQKNKTIGDLLQVLQEMGHRRAIHLITNYGAVLSPSEKSYQEG
GFPNILFKETANVTVDNVLIPEHNEKGVLLKSSISFQNIIEGTRNFHKDF
LIGEGEIFEVYRVEIQNLTYAVKLFKQEKKMQCKKHWKRFLSELEVLLLF
HHPNILELAAYFTETEKFCLIYPYMRNGTLFDRLQCVGDTAPLPWHIRIG
ILIGISKAIHYLHNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRS
HLEHQSCTINMTSSSSKHLWYMPEEYIRQGKLSIKTDVYSFGIVIMEVLT
GCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLF
CLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPL
FLENVPSIPVEDDESQNNNLLPSDEGLRIDRMTQKTPFECSQSEVMFLSL
DKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSEAPG
HSCRSRPVESSCSSKFSWDEYEQYKKE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4712
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.