cDNA Clone ASK1

• Synonyms: MAP3K5
• Plasmid: pGEX6P-1-ASK1 cat
• Parent Plasmid: pGEX6P1
• Expressed: GST-ASK1 670-950
• Genbank: NM_005923
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTTGCTGG
AGTATGACTATGAATATGATGAAAATGGTGACAGAGTCGTTTTAGGAAAA
GGCACTTATGGGATAGTCTACGCAGGTCGGGACTTGAGCAACCAAGTCAG
AATTGCTATTAAGGAAATCCCAGAGAGAGACAGCAGATACTCTCAGCCCC
TGCATGAAGAAATAGCATTGCATAAACACCTGAAGCACAAAAATATTGTC
CAGTATCTGGGCTCTTTCAGTGAGAATGGTTTCATTAAAATCTTCATGGA
GCAGGTCCCTGGAGGAAGTCTTTCTGCTCTCCTTCGTTCCAAATGGGGTC
CATTAAAAGACAATGAGCAAACAATTGGCTTTTATACAAAGCAAATACTG
GAAGGATTAAAATATCTCCATGACAATCAGATAGTTCACCGGGACATAAA
GGGTGACAATGTGTTGATTAATACCTACAGTGGTGTTCTCAAGATCTCTG
ACTTCGGAACATCAAAGAGGCTTGCTGGCATAAACCCCTGTACTGAAACT
TTTACTGGTACCCTCCAGTATATGGCACCAGAAATAATAGATAAAGGACC
AAGAGGCTACGGAAAAGCAGCAGACATCTGGTCTCTGGGCTGTACAATCA
TTGAAATGGCCACAGGAAAACCCCCATTTTATGAACTGGGAGAACCACAA
GCAGCTATGTTCAAGGTGGGAATGTTTAAAGTCCACCCTGAGATCCCAGA
GTCCATGTCTGCAGAGGCCAAGGCATTCATACTGAAATGTTTTGAACCAG
ATCCTGACAAGAGAGCCTGTGCTAACGACTTGCTTGTTGATGAGTTTTTA
AAAGTTTCAAGCAAAAAGAAAAAGACACAACCTAAGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLLEYDYEYDENGDRVVLGK
GTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIV
QYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQIL
EGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTET
FTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQ
AAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFL
KVSSKKKKTQPK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4710
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.