cDNA Clone MHC2TA

• Plasmid: pEBG6P-1 MHC2TA
• Parent Plasmid: pEBG6P
• Expressed: GST-MHC2TA
• Genbank: NM_000246
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTATGCGTTGCCTGGCTCCACGCCCTGCTGGGTCC
TACCTGTCAGAGCCCCAAGGCAGCTCACAGTGTGCCACCATGGAGTTGGG
GCCCCTAGAAGGTGGCTACCTGGAGCTTCTTAACAGCGATGCTGACCCCC
TGTGCCTCTACCACTTCTATGACCAGATGGACCTGGCTGGAGAAGAAGAG
ATTGAGCTCTACTCAGAACCCGACACAGACACCATCAACTGCGACCAGTT
CAGCAGGCTGTTGTGTGACATGGAAGGTGATGAAGAGACCAGGGAGGCTT
ATGCCAATATCGCGGAACTGGACCAGTATGTCTTCCAGGACTCCCAGCTG
GAGGGCCTGAGCAAGGACATTTTCAAGCACATAGGACCAGATGAAGTGAT
CGGTGAGAGTATGGAGATGCCAGCAGAAGTTGGGCAGAAAAGTCAGAAAA
GACCCTTCCCAGAGGAGCTTCCGGCAGACCTGAAGCACTGGAAGCCAGCT
GAGCCCCCCACTGTGGTGACTGGCAGTCTCCTAGTGGGACCAGTGAGCGA
CTGCTCCACCCTGCCCTGCCTGCCACTGCCTGCGCTGTTCAACCAGGAGC
CAGCCTCCGGCCAGATGCGCCTGGAGAAAACCGACCAGATTCCCATGCCT
TTCTCCAGTTCCTCGTTGAGCTGCCTGAATCTCCCTGAGGGACCCATCCA
GTTTGTCCCCACCATCTCCACTCTGCCCCATGGGCTCTGGCAAATCTCTG
AGGCTGGAACAGGGGTCTCCAGTATATTCATCTACCATGGTGAGGTGCCC
CAGGCCAGCCAAGTACCCCCTCCCAGTGGATTCACTGTCCACGGCCTCCC
AACATCTCCAGACCGGCCAGGCTCCACCAGCCCCTTCGCTCCATCAGCCA
CTGACCTGCCCAGCATGCCTGAACCTGCCCTGACCTCCCGAGCAAACATG
ACAGAGCACAAGACGTCCCCCACCCAATGCCCGGCAGCTGGAGAGGTCTC
CAACAAGCTTCCAAAATGGCCTGAGCCGGTGGAGCAGTTCTACCGCTCAC
TGCAGGACACGTATGGTGCCGAGCCCGCAGGCCCGGATGGCATCCTAGTG
GAGGTGGATCTGGTGCAGGCCAGGCTGGAGAGGAGCAGCAGCAAGAGCCT
GGAGCGGGAACTGGCCACCCCGGACTGGGCAGAACGGCAGCTGGCCCAAG
GAGGCCTGGCTGAGGTGCTGTTGGCTGCCAAGGAGCACCGGCGGCCGCGT
GAGACACGAGTGATTGCTGTGCTGGGCAAAGCTGGTCAGGGCAAGAGCTA
TTGGGCTGGGGCAGTGAGCCGGGCCTGGGCTTGTGGCCGGCTTCCCCAGT
ACGACTTTGTCTTCTCTGTCCCCTGCCATTGCTTGAACCGTCCGGGGGAT
GCCTATGGCCTGCAGGATCTGCTCTTCTCCCTGGGCCCACAGCCACTCGT
GGCGGCCGATGAGGTTTTCAGCCACATCTTGAAGAGACCTGACCGCGTTC
TGCTCATCCTAGACGCCTTCGAGGAGCTGGAAGCGCAAGATGGCTTCCTG
CACAGCACGTGCGGACCGGCACCGGCGGAGCCCTGCTCCCTCCGGGGGCT
GCTGGCCGGCCTTTTCCAGAAGAAGCTGCTCCGAGGTTGCACCCTCCTCC
TCACAGCCCGGCCCCGGGGCCGCCTGGTCCAGAGCCTGAGCAAGGCCGAC
GCCCTATTTGAGCTGTCCGGCTTCTCCATGGAGCAGGCCCAGGCATACGT
GATGCGCTACTTTGAGAGCTCAGGGATGACAGAGCACCAAGACAGAGCCC
TGACGCTCCTCCGGGACCGGCCACTTCTTCTCAGTCACAGCCACAGCCCT
ACTTTGTGCCGGGCAGTGTGCCAGCTCTCAGAGGCCCTGCTGGAGCTTGG
GGAGGACGCCAAGCTGCCCTCCACGCTCACGGGACTCTATGTCGGCCTGC
TGGGCCGTGCAGCCCTCGACAGCCCCCCCGGGGCCCTGGCAGAGCTGGCC
AAGCTGGCCTGGGAGCTGGGCCGCAGACATCAAAGTACCCTACAGGAGGA
CCAGTTCCCATCCGCAGACGTGAGGACCTGGGCGATGGCCAAAGGCTTAG
TCCAACACCCACCGCGGGCCGCAGAGTCCGAGCTGGCCTTCCCCAGCTTC
CTCCTGCAATGCTTCCTGGGGGCCCTGTGGCTGGCTCTGAGTGGCGAAAT
CAAGGACAAGGAGCTCCCGCAGTACCTAGCATTGACCCCAAGGAAGAAGA
GGCCCTATGACAACTGGCTGGAGGGCGTGCCACGCTTTCTGGCTGGGCTG
ATCTTCCAGCCTCCCGCCCGCTGCCTGGGAGCCCTACTCGGGCCATCGGC
GGCTGCCTCGGTGGACAGGAAGCAGAAGGTGCTTGCGAGGTACCTGAAGC
GGCTGCAGCCGGGGACACTGCGGGCGCGGCAGCTGCTTGAGCTGCTGCAC
TGCGCCCACGAGGCCGAGGAGGCTGGAATTTGGCAGCACGTGGTACAGGA
GCTCCCCGGCCGCCTCTCTTTTCTGGGCACCCGCCTCACGCCTCCTGATG
CACATGTACTGGGCAAGGCCTTGGAGGCGGCGGGCCAAGACTTCTCCCTG
GACCTCCGCAGCACTGGCATTTGCCCCTCTGGATTGGGGAGCCTCGTGGG
ACTCAGCTGTGTCACCCGTTTCAGGGCTGCCTTGAGCGACACGGTGGCGC
TGTGGGAGTCCCTGCGGCAGCATGGGGAGACCAAGCTACTTCAGGCAGCA
GAGGAGAAGTTCACCATCGAGCCTTTCAAAGCCAAGTCCCTGAAGGATGT
GGAAGACCTGGGAAAGCTTGTGCAGACTCAGAGGACGAGAAGTTCCTCGG
AAGACACAGCTGGGGAGCTCCCTGCTGTTCGGGACCTAAAGAAACTGGAG
TTTGCGCTGGGCCCTGTCTCAGGCCCCCAGGCTTTCCCCAAACTGGTGCG
GATCCTCACGGCCTTTTCCTCCCTGCAGCATCTGGACCTGGATGCGCTGA
GTGAGAACAAGATCGGGGACGAGGGTGTCTCGCAGCTCTCAGCCACCTTC
CCCCAGCTGAAGTCCTTGGAAACCCTCAATCTGTCCCAGAACAACATCAC
TGACCTGGGTGCCTACAAACTCGCCGAGGCCCTGCCTTCGCTCGCTGCAT
CCCTGCTCAGGCTAAGCTTGTACAATAACTGCATCTGCGACGTGGGAGCC
GAGAGCTTGGCTCGTGTGCTTCCGGACATGGTGTCCCTCCGGGTGATGGA
CGTCCAGTACAACAAGTTCACGGCTGCCGGGGCCCAGCAGCTCGCTGCCA
GCCTTCGGAGGTGTCCTCATGTGGAGACGCTGGCGATGTGGACGCCCACC
ATCCCATTCAGTGTCCAGGAACACCTGCAACAACAGGATTCACGGATCAG
CCTGAGATGAACTAGT

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMRCLAPRPAGS
YLSEPQGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEE
IELYSEPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQL
EGLSKDIFKHIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPA
EPPTVVTGSLLVGPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMP
FSSSSLSCLNLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVP
QASQVPPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANM
TEHKTSPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILV
EVDLVQARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPR
ETRVIAVLGKAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGD
AYGLQDLLFSLGPQPLVAADEVFSHILKRPDRVLLILDAFEELEAQDGFL
HSTCGPAPAEPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKAD
ALFELSGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSP
TLCRAVCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPPGALAELA
KLAWELGRRHQSTLQEDQFPSADVRTWAMAKGLVQHPPRAAESELAFPSF
LLQCFLGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGL
IFQPPARCLGALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLH
CAHEAEEAGIWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSL
DLRSTGICPSGLGSLVGLSCVTRFRAALSDTVALWESLRQHGETKLLQAA
EEKFTIEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLE
FALGPVSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATF
PQLKSLETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGA
ESLARVLPDMVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPT
IPFSVQEHLQQQDSRISLR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4698
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.