cDNA Clone IRAK4

• Plasmid: pEBG6P-1 IRAK4 D329A
• Parent Plasmid: pEBG6P
• Expressed: GST-IRAK4 D329A
• Genbank: AAH13316
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGAACAAACCCATAACACCATCAACATATGTGCGCTGC
CTCAATGTTGGACTAATTAGGAAGCTGTCAGATTTTATTGATCCTCAAGA
AGGATGGAAGAAGTTAGCTGTAGCTATTAAAAAACCATCTGGTGATGATA
GATACAATCAGTTTCACATAAGGAGATTTGAAGCATTACTTCAAACTGGA
AAAAGTCCCACTTCTGAATTACTGTTTGACTGGGGCACCACAAATTGCAC
AGTTGGTGATCTTGTGGATCTTTTGATCCAAAATGAATTTTTTGCTCCTG
CAAGTCTTTTGCTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCT
TCTAAAGAAGCTATAACAGTTCAGCAAAAACAGATGCCTTTCTGTGACAA
AGACAGGACATTGATGACACCTGTGCAGAATCTTGAACAAAGCTATATGC
CACCTGACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTTAGTGATACA
CGTTTTCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTT
TGATGAACGACCCATTTCTGTTGGTGGTAATAAAATGGGAGAGGGAGGAT
TTGGAGTTGTATATAAAGGCTACGTAAATAACACAACTGTGGCAGTGAAG
AAGCTTGCAGCAATGGTTGACATTACTACTGAAGAACTGAAACAGCAGTT
TGATCAAGAAATAAAAGTAATGGCAAAGTGTCAACATGAAAACTTAGTAG
AACTACTTGGTTTCTCAAGTGATGGAGATGACCTCTGCTTAGTATATGTT
TACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTAC
TCCACCACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTA
ATGGCATCAATTTTCTACATGAAAATCATCATATTCATAGAGATATTAAA
AGTGCAAATATCTTACTGGATGAAGCTTTTACTGCTAAAATATCTGCCTT
TGGCCTTGCACGGGCTTCTGAGAAGTTTGCCCAGACAGTCATGACTAGCA
GAATTGTGGGAACAACAGCTTATATGGCACCAGAAGCTTTGCGTGGAGAA
ATAACACCCAAATCTGATATTTACAGCTTTGGTGTGGTTTTACTAGAAAT
AATAACTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGC
TAGATATTAAAGAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTAT
ATTGATAAAAAGATGAATGATGCTGATTCCACTTCAGTTGAAGCTATGTA
CTCTGTTGCTAGTCAATGTCTGCATGAAAAGAAAAATAAGAGACCAGACA
TTAAGAAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCTTAAGCGGCC
GCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNKPITPSTYVRC
LNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTG
KSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLP
SKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDT
RFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVK
KLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYV
YMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIK
SANILLDEAFTAKISAFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGE
ITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDY
IDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4683
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.