cDNA Clone PMK1

• Plasmid: pGEX4T-1-PMK1
• Parent Plasmid: pGEX4T1
• Expressed: GST-PMK1
• Genbank: XM_364720
• Tag: GST
• Species: Magnaporthe grisea

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCCCCGGAATTCATGTCTCGCGCCA
ATCCACCAAGCAACTCTTCGGGGTCTCGCAAGATCTCATTCAACGTAAGC
GAGCAATACGACATCCAGGATGTCGTGGGCGAGGGCGCTTATGGAGTCGT
TTGCTCTGCGATCCATAAGCCTTCGGGCCAAAAGGTTGCCATAAAGAAGA
TCACCCCGTTCGATCATTCTATGTTCTGCCTACGAACCCTCCGCGAGATG
AAGCTGCTCCGGTACTTCAACCACGAGAACATCATTTCCATCCTCGACAT
CCAGAAGCCCCGAAGCTACGAGACCTTCAACGAGGTGTACCTGATCCAGG
AGCTCATGGAGACTGACATGCACCGTGTCATCCGCACCCAAGACCTATCC
GACGATCACTGCCAGTACTTCATCTACCAGACTCTGCGTGCTCTCAAGGC
AATGCACTCGGCAAACGTGCTTCACCGTGATCTGAAGCCCTCGAATCTCC
TTCTCAACGCCAACTGTGATTTGAAGGTTTGCGATTTTGGTCTGGCTCGT
TCTGCCGCCTCGCAAGAGGACAACTCGGGTTTCATGACAGAATACGTCGC
TACGCGTTGGTACCGTGCTCCCGAGATCATGTTGACCTTTAAGGAGTACA
CCAAGGCTATTGACGTGTGGTCTGTTGGCTGCATTCTGGCTGAAATGCTC
AGCGGAAAGCCTTTGTTCCCCGGAAAGGACTACCACCACCAGCTCACGCT
GATTCTTGACGTTCTCGGCACACCCACCATGGAGGACTACTATGGTATCA
AGTCCCGCCGTGCAAGGGAGTACATTCGCTCGCTGCCGTTCAAGAAAAAG
GTTCCTTTCAGGACACTCTTCCCCAAGACTTCGGATCTCGCCTTGGACCT
GTTGGAGAAGTTGCTTGCCTTTAACCCCGTCAAGCGCATCACCGTCGAGG
AAGCTTTGAAGCACCCTTACCTTGAGCCGTACCATGACCCCGATGATGAA
CCCACCGCACCGCCGATTCCCGAGGAGTTCTTTGATTTCGATAAGCACAA
GGATAACCTGAGCAAAGAGCAGCTCAAGCAGTTCATCTACCAGGAAATTA
TGCGGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPEFMSRANPPSNSSGSRKISFNVS
EQYDIQDVVGEGAYGVVCSAIHKPSGQKVAIKKITPFDHSMFCLRTLREM
KLLRYFNHENIISILDIQKPRSYETFNEVYLIQELMETDMHRVIRTQDLS
DDHCQYFIYQTLRALKAMHSANVLHRDLKPSNLLLNANCDLKVCDFGLAR
SAASQEDNSGFMTEYVATRWYRAPEIMLTFKEYTKAIDVWSVGCILAEML
SGKPLFPGKDYHHQLTLILDVLGTPTMEDYYGIKSRRAREYIRSLPFKKK
VPFRTLFPKTSDLALDLLEKLLAFNPVKRITVEEALKHPYLEPYHDPDDE
PTAPPIPEEFFDFDKHKDNLSKEQLKQFIYQEIMR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4673
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.