cDNA Clone IGF1-R

• Plasmid: pEBG6P-1 IGF1-R 954-1367
• Parent Plasmid: pEBG6P
• Expressed: GST-IGF1-R 954-1367
• Genbank: NM_000875
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGCTGTACGTCTTCCATAGAAAGAGAAATAACAGCAGG
CTGGGGAATGGAGTGCTGTATGCCTCTGTGAACCCGGAGTACTTCAGCGC
TGCTGATGTGTACGTTCCTGATGAGTGGGAGGTGGCTCGGGAGAAGATCA
CCATGAGCCGGGAACTTGGGCAGGGGTCGTTTGGGATGGTCTATGAAGGA
GTTGCCAAGGGTGTGGTGAAAGATGAACCTGAAACCAGAGTGGCCATTAA
AACAGTGAACGAGGCCGCAAGCATGCGTGAAAGGATTGAGTTTCTCAACG
AAGCTTCTGTGATGAAGGAGTTCAATTGTCACCATGTGGTGCGATTGCTG
GGTGTGGTGTCCCAAGGCCAGCCAACACTGGTCATCATGGAACTGATGAC
ACGGGGCGATCTCAAAAGTTATCTCCGGTCTCTGAGGCCAGAAATGGAGA
ATAATCCAGTCCTAGCACCTCCAAGCCTGAGCAAGATGATTCAGATGGCC
GGAGAGATTGCAGACGGCATGGCATACCTCAACGCCAATAAGTTCGTCCA
CAGAGACCTTGCTGCCCGGAATTGCATGGTAGCCGAAGATTTCACAGTCA
AAATCGGAGATTTTGGTATGACGCGAGATATCTATGAGACAGACTATTAC
CGGAAAGGAGGGAAAGGGCTGCTGCCCGTGCGCTGGATGTCTCCTGAGTC
CCTCAAGGATGGAGTCTTCACCACTTACTCGGACGTCTGGTCCTTCGGGG
TCGTCCTCTGGGAGATCGCCACACTGGCCGAGCAGCCCTACCAGGGCTTG
TCCAACGAGCAAGTCCTTCGCTTCGTCATGGAGGGCGGCCTTCTGGACAA
GCCAGACAACTGTCCTGACATGCTGTTTGAACTGATGCGCATGTGCTGGC
AGTATAACCCCAAGATGAGGCCTTCCTTCCTGGAGATCATCAGCAGCATC
AAAGAGGAGATGGAGCCTGGCTTCCGGGAGGTCTCCTTCTACTACAGCGA
GGAGAACAAGCTGCCCGAGCCGGAGGAGCTGGACCTGGAGCCAGAGAACA
TGGAGAGCGTCCCCCTGGACCCCTCGGCCTCCTCGTCCTCCCTGCCACTG
CCCGACAGACACTCAGGACACAAGGCCGAGAACGGCCCCGGCCCTGGGGT
GCTGGTCCTCCGCGCCAGCTTCGACGAGAGACAGCCTTACGCCCACATGA
ACGGGGGCCGCAAGAACGAGCGGGCCTTGCCGCTGCCCCAGTCTTCGACC
TGCTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMLYVFHRKRNNSR
LGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEG
VAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLL
GVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMA
GEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYY
RKGGKGLLPVRWMSPESLKDGVFTTYSDVWSFGVVLWEIATLAEQPYQGL
SNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSI
KEEMEPGFREVSFYYSEENKLPEPEELDLEPENMESVPLDPSASSSSLPL
PDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSST
C*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4669
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.