cDNA Clone IGF1-R

• Plasmid: pGEX6P-1-IGF1-R 954-1367
• Parent Plasmid: pGEX6P1
• Expressed: GST-IGF1-R 954-1367
• Genbank: NM_000875
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGCTGT
ACGTCTTCCATAGAAAGAGAAATAACAGCAGGCTGGGGAATGGAGTGCTG
TATGCCTCTGTGAACCCGGAGTACTTCAGCGCTGCTGATGTGTACGTTCC
TGATGAGTGGGAGGTGGCTCGGGAGAAGATCACCATGAGCCGGGAACTTG
GGCAGGGGTCGTTTGGGATGGTCTATGAAGGAGTTGCCAAGGGTGTGGTG
AAAGATGAACCTGAAACCAGAGTGGCCATTAAAACAGTGAACGAGGCCGC
AAGCATGCGTGAAAGGATTGAGTTTCTCAACGAAGCTTCTGTGATGAAGG
AGTTCAATTGTCACCATGTGGTGCGATTGCTGGGTGTGGTGTCCCAAGGC
CAGCCAACACTGGTCATCATGGAACTGATGACACGGGGCGATCTCAAAAG
TTATCTCCGGTCTCTGAGGCCAGAAATGGAGAATAATCCAGTCCTAGCAC
CTCCAAGCCTGAGCAAGATGATTCAGATGGCCGGAGAGATTGCAGACGGC
ATGGCATACCTCAACGCCAATAAGTTCGTCCACAGAGACCTTGCTGCCCG
GAATTGCATGGTAGCCGAAGATTTCACAGTCAAAATCGGAGATTTTGGTA
TGACGCGAGATATCTATGAGACAGACTATTACCGGAAAGGAGGGAAAGGG
CTGCTGCCCGTGCGCTGGATGTCTCCTGAGTCCCTCAAGGATGGAGTCTT
CACCACTTACTCGGACGTCTGGTCCTTCGGGGTCGTCCTCTGGGAGATCG
CCACACTGGCCGAGCAGCCCTACCAGGGCTTGTCCAACGAGCAAGTCCTT
CGCTTCGTCATGGAGGGCGGCCTTCTGGACAAGCCAGACAACTGTCCTGA
CATGCTGTTTGAACTGATGCGCATGTGCTGGCAGTATAACCCCAAGATGA
GGCCTTCCTTCCTGGAGATCATCAGCAGCATCAAAGAGGAGATGGAGCCT
GGCTTCCGGGAGGTCTCCTTCTACTACAGCGAGGAGAACAAGCTGCCCGA
GCCGGAGGAGCTGGACCTGGAGCCAGAGAACATGGAGAGCGTCCCCCTGG
ACCCCTCGGCCTCCTCGTCCTCCCTGCCACTGCCCGACAGACACTCAGGA
CACAAGGCCGAGAACGGCCCCGGCCCTGGGGTGCTGGTCCTCCGCGCCAG
CTTCGACGAGAGACAGCCTTACGCCCACATGAACGGGGGCCGCAAGAACG
AGCGGGCCTTGCCGCTGCCCCAGTCTTCGACCTGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLYVFHRKRNNSRLGNGVL
YASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVV
KDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQG
QPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADG
MAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKG
LLPVRWMSPESLKDGVFTTYSDVWSFGVVLWEIATLAEQPYQGLSNEQVL
RFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSIKEEMEP
GFREVSFYYSEENKLPEPEELDLEPENMESVPLDPSASSSSLPLPDRHSG
HKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4668
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.