cDNA Clone ERN1

• Plasmid: pCMV5-FLAG-1 ERN1
• Parent Plasmid: pCMV5
• Expressed: FLAG-ERN1
• Genbank: NM_001433
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCCCGGAATTCAT
GCCGGCCCGGCGGCTGCTGCTGCTGCTGACGCTGCTGCTGCCCGGCCTCG
GGATTTTTGGAAGTACCAGCACAGTGACGCTTCCTGAAACCTTGTTGTTT
GTGTCAACGCTGGATGGAAGTTTGCATGCTGTCAGCAAGAGGACAGGCTC
AATCAAATGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACATG
TGGAAGAGCCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTATACG
CTTGGAAGCAAGAATAATGAAGGCCTGACGAAACTTCCTTTTACCATCCC
AGAATTGGTGCAGGCATCCCCATGCCGAAGTTCAGATGGAATCCTCTACA
TGGGTAAAAAGCAGGACATCTGGTATGTTATTGACCTCCTGACCGGAGAG
AAGCAGCAGACTTTGTCATCGGCCTTTGCAGATAGTCTCTGCCCATCAAC
CTCTCTTCTGTATCTTGGGCGAACAGAATACACCATCACCATGTACGACA
CCAAAACCCGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGCGGCC
TCACTGCCTGAGGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAA
TGGTGATGGGCTGGTGGTGACTGTGGACAGTGAATCTGGGGACGTCCTGT
GGATCCAAAACTACGCCTCCCCTGTGGTGGCCTTTTATGTCTGGCAGCGG
GAGGGTCTGAGGAAGGTGATGCACATCAATGTCGCTGTGGAGACCCTGCG
CTATCTGACCTTCATGTCTGGGGAGGTGGGGCGCATCACAAAGTGGAAGT
ACCCGTTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTGACGCCCACTCTG
TATGTTGGGAAATACTCTACCAGCCTCTATGCCTCTCCCTCAATGGTACA
CGAGGGGGTTGCTGTCGTGCCCCGCGGCAGCACACTTCCTTTGCTGGAAG
GGCCCCAGACTGATGGCGTCACCATCGGGGACAAGGGGGAGTGTGTGATC
ACGCCCAGCACGGACGTCAAGTTTGATCCCGGACTCAAAAGCAAGAACAA
GCTCAACTACTTGAGGAATTACTGGCTTCTGATAGGACACCATGAAACCC
CACTGTCTGCGTCTACCAAGATGCTGGAGAGATTTCCCAACAATCTACCC
AAACATCGGGAAAATGTGATTCCTGCTGATTCAGAGAAAAAGAGCTTTGA
GGAAGTTATCAACCTGGTTGACCAGACTTCAGAAAACGCACCTACCACCG
TGTCTCGGGATGTGGAGGAGAAGCCCGCCCATGCCCCTGCCCGGCCCGAG
GCCCCCGTGGACTCCATGCTTAAGGACATGGCTACCATCATCCTGAGCAC
CTTCCTGCTGATTGGCTGGGTGGCCTTCATCATCACCTATCCCCTGAGCA
TGCATCAGCAGCAGCAGCTCCAGCACCAGCAGTTCCAGAAGGAACTGGAG
AAGATCCAGCTCCTGCAGCAGCAGCAGCAGCAGCTGCCCTTCCACCCACC
TGGAGACACGGCTCAGGACGGCGAGCTCCTGGACACGTCTGGCCCGTACT
CAGAGAGCTCGGGCACCAGCAGCCCCAGCACGTCCCCCAGGGCCTCCAAC
CACTCACTCTGCTCCGGCAGCTCTGCCTCCAAGGCTGGCAGCAGCCCCTC
CCTGGAACAAGACGATGGAGATGAGGAAACCAGCGTGGTGATAGTTGGGA
AAATTTCCTTCTGTCCCAAGGATGTCCTGGGCCATGGAGCTGAGGGCACA
ATTGTGTACCGGGGCATGTTTGACAACCGCGACGTGGCCGTGAAGAGGAT
CCTCCCCGAGTGTTTTAGCTTCGCAGACCGTGAGGTCCAGCTGTTGCGAG
AATCGGATGAGCACCCGAACGTGATCCGCTACTTCTGCACGGAGAAGGAC
CGGCAATTCCAGTACATTGCCATCGAGCTGTGTGCAGCCACCCTGCAAGA
GTATGTGGAGCAGAAGGACTTTGCGCATCTCGGCCTGGAGCCCATCACCT
TGCTGCAGCAGACCACCTCGGGCCTGGCCCACCTCCACTCCCTCAACATC
GTTCACAGAGACCTAAAGCCACACAACATCCTCATATCCATGCCCAATGC
ACACGGCAAGATCAAGGCCATGATCTCCGACTTTGGCCTCTGCAAGAAGC
TGGCAGTGGGCAGACACAGTTTCAGCCGCCGATCTGGGGTGCCTGGCACA
GAAGGCTGGATCGCTCCAGAGATGCTGAGCGAAGACTGTAAGGAGAACCC
TACCTACACGGTGGACATCTTTTCTGCAGGCTGCGTCTTTTACTACGTCA
TCTCTGAGGGCAGCCACCCTTTTGGCAAGTCCCTGCAGCGGCAGGCCAAC
ATCCTCCTGGGTGCCTGCAGCCTTGACTGCTTGCACCCAGAGAAGCACGA
AGACGTCATTGCACGTGAATTGATAGAGAAGATGATTGCGATGGATCCTC
AGAAACGCCCCTCGGCGAAGCACGTGCTCAAACACCCGTTCTTCTGGAGC
CTAGAGAAGCAGCTCCAGTTCTTCCAGGACGTGAGCGACAGAATAGAAAA
GGAATCCCTGGATGGCCCGATCGTGAAGCAGTTAGAGAGAGGCGGGAGAG
CCGTGGTGAAGATGGACTGGCGGGAGAACATCACTGACCCCCTCCAGACA
GACCTGCGTAAATTCAGGACCTATAAAGGTGGTTCTGTCAGAGATCTCCT
CCGAGCCATGAGAAATAAGAAGCACCACTACCGGGAGCTGCCTGCAGAGG
TGCGGGAGACGCTGGGGACCCTCCCCGACGACTTCGTGTGCTACTTCACA
TCTCGCTTCCCCCACCTCCTCGCACACACCTACCGGGCCATGGAGCTGTG
CAGCCACGAGAGACTCTTCCAGCCCTACTACTTCCACGAGCCCCCAGAGC
CCCAGCCCCCAGTGACTCCAGACGCCCTCTGAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSPEFMPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVS
TLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLG
SKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQ
QTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASL
PEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREG
LRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYV
GKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITP
STDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKH
RENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAP
VDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKI
QLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHS
LCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIV
YRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQ
FQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVH
RDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEG
WIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANIL
LGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLE
KQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITDPLQTDL
RKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGTLPDDFVCYFTSR
FPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4660
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG.; Has 2 SNPS: V880D S924T - seen in other published sequences.
• Low Temp: No