cDNA Clone ERN1

• Plasmid: pGEX6P-1-ERN1
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERN1
• Genbank: NM_001433
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCATGCCGGCCCGGCGGCTGCTGCTGCTGCTGACGCTGCTGCTGCCCGGC
CTCGGGATTTTTGGAAGTACCAGCACAGTGACGCTTCCTGAAACCTTGTT
GTTTGTGTCAACGCTGGATGGAAGTTTGCATGCTGTCAGCAAGAGGACAG
GCTCAATCAAATGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACA
CATGTGGAAGAGCCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTA
TACGCTTGGAAGCAAGAATAATGAAGGCCTGACGAAACTTCCTTTTACCA
TCCCAGAATTGGTGCAGGCATCCCCATGCCGAAGTTCAGATGGAATCCTC
TACATGGGTAAAAAGCAGGACATCTGGTATGTTATTGACCTCCTGACCGG
AGAGAAGCAGCAGACTTTGTCATCGGCCTTTGCAGATAGTCTCTGCCCAT
CAACCTCTCTTCTGTATCTTGGGCGAACAGAATACACCATCACCATGTAC
GACACCAAAACCCGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGC
GGCCTCACTGCCTGAGGACGACGTGGACTACAAGATGTCCCACTTTGTGT
CCAATGGTGATGGGCTGGTGGTGACTGTGGACAGTGAATCTGGGGACGTC
CTGTGGATCCAAAACTACGCCTCCCCTGTGGTGGCCTTTTATGTCTGGCA
GCGGGAGGGTCTGAGGAAGGTGATGCACATCAATGTCGCTGTGGAGACCC
TGCGCTATCTGACCTTCATGTCTGGGGAGGTGGGGCGCATCACAAAGTGG
AAGTACCCGTTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTGACGCCCAC
TCTGTATGTTGGGAAATACTCTACCAGCCTCTATGCCTCTCCCTCAATGG
TACACGAGGGGGTTGCTGTCGTGCCCCGCGGCAGCACACTTCCTTTGCTG
GAAGGGCCCCAGACTGATGGCGTCACCATCGGGGACAAGGGGGAGTGTGT
GATCACGCCCAGCACGGACGTCAAGTTTGATCCCGGACTCAAAAGCAAGA
ACAAGCTCAACTACTTGAGGAATTACTGGCTTCTGATAGGACACCATGAA
ACCCCACTGTCTGCGTCTACCAAGATGCTGGAGAGATTTCCCAACAATCT
ACCCAAACATCGGGAAAATGTGATTCCTGCTGATTCAGAGAAAAAGAGCT
TTGAGGAAGTTATCAACCTGGTTGACCAGACTTCAGAAAACGCACCTACC
ACCGTGTCTCGGGATGTGGAGGAGAAGCCCGCCCATGCCCCTGCCCGGCC
CGAGGCCCCCGTGGACTCCATGCTTAAGGACATGGCTACCATCATCCTGA
GCACCTTCCTGCTGATTGGCTGGGTGGCCTTCATCATCACCTATCCCCTG
AGCATGCATCAGCAGCAGCAGCTCCAGCACCAGCAGTTCCAGAAGGAACT
GGAGAAGATCCAGCTCCTGCAGCAGCAGCAGCAGCAGCTGCCCTTCCACC
CACCTGGAGACACGGCTCAGGACGGCGAGCTCCTGGACACGTCTGGCCCG
TACTCAGAGAGCTCGGGCACCAGCAGCCCCAGCACGTCCCCCAGGGCCTC
CAACCACTCACTCTGCTCCGGCAGCTCTGCCTCCAAGGCTGGCAGCAGCC
CCTCCCTGGAACAAGACGATGGAGATGAGGAAACCAGCGTGGTGATAGTT
GGGAAAATTTCCTTCTGTCCCAAGGATGTCCTGGGCCATGGAGCTGAGGG
CACAATTGTGTACCGGGGCATGTTTGACAACCGCGACGTGGCCGTGAAGA
GGATCCTCCCCGAGTGTTTTAGCTTCGCAGACCGTGAGGTCCAGCTGTTG
CGAGAATCGGATGAGCACCCGAACGTGATCCGCTACTTCTGCACGGAGAA
GGACCGGCAATTCCAGTACATTGCCATCGAGCTGTGTGCAGCCACCCTGC
AAGAGTATGTGGAGCAGAAGGACTTTGCGCATCTCGGCCTGGAGCCCATC
ACCTTGCTGCAGCAGACCACCTCGGGCCTGGCCCACCTCCACTCCCTCAA
CATCGTTCACAGAGACCTAAAGCCACACAACATCCTCATATCCATGCCCA
ATGCACACGGCAAGATCAAGGCCATGATCTCCGACTTTGGCCTCTGCAAG
AAGCTGGCAGTGGGCAGACACAGTTTCAGCCGCCGATCTGGGGTGCCTGG
CACAGAAGGCTGGATCGCTCCAGAGATGCTGAGCGAAGACTGTAAGGAGA
ACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGCGTCTTTTACTAC
GTCATCTCTGAGGGCAGCCACCCTTTTGGCAAGTCCCTGCAGCGGCAGGC
CAACATCCTCCTGGGTGCCTGCAGCCTTGACTGCTTGCACCCAGAGAAGC
ACGAAGACGTCATTGCACGTGAATTGATAGAGAAGATGATTGCGATGGAT
CCTCAGAAACGCCCCTCGGCGAAGCACGTGCTCAAACACCCGTTCTTCTG
GAGCCTAGAGAAGCAGCTCCAGTTCTTCCAGGACGTGAGCGACAGAATAG
AAAAGGAATCCCTGGATGGCCCGATCGTGAAGCAGTTAGAGAGAGGCGGG
AGAGCCGTGGTGAAGATGGACTGGCGGGAGAACATCACTGACCCCCTCCA
GACAGACCTGCGTAAATTCAGGACCTATAAAGGTGGTTCTGTCAGAGATC
TCCTCCGAGCCATGAGAAATAAGAAGCACCACTACCGGGAGCTGCCTGCA
GAGGTGCGGGAGACGCTGGGGACCCTCCCCGACGACTTCGTGTGCTACTT
CACATCTCGCTTCCCCCACCTCCTCGCACACACCTACCGGGCCATGGAGC
TGTGCAGCCACGAGAGACTCTTCCAGCCCTACTACTTCCACGAGCCCCCA
GAGCCCCAGCCCCCAGTGACTCCAGACGCCCTCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMPARRLLLLLTLLLPG
LGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPT
HVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGIL
YMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMY
DTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDV
LWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKW
KYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLL
EGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHE
TPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPT
TVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPL
SMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGP
YSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIV
GKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLL
RESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPI
TLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCK
KLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYY
VISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMD
PQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGG
RAVVKMDWRENITDPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPA
EVRETLGTLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPP
EPQPPVTPDAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4658
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST. Has 2 SNPS: V880D S924T - seen in other published sequences
• Low Temp: No