cDNA Clone Optineurin

• Plasmid: pGEX6P-1-Optineurin E478A
• Parent Plasmid: pGEX6P1
• Expressed: GST-Optineurin E478A
• Genbank: AF420371
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCCC
ATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGC
ACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTAC
CCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACC
AGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTT
GAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTT
TGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATG
AGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAA
AGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGC
GGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAG
AGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAAC
TCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGG
AGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGA
GAACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCA
GATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCT
CCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTG
CACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGT
AATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAA
TGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGA
ACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAA
CTCAGCGAAGCTGAGCTAATGAAGAAGAGGCTTCAAGAAAAGTGTCAGGC
CCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAG
AGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTA
TCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATT
AACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATG
CATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGAC
AGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGC
TCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCA
AGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAA
GTTTACTGTTCTGATTTTCATGCTGCAAGAGCAGCGAGAGAGAAAATTCA
TGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGA
ATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGT
CGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCA
AAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTC
ATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAG
ATTCACGTGATGGATTGCATCATTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSHQPLSCLTEKEDSPSES
TGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRF
EELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSE
RSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLN
SSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSA
DGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTS
NRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTK
LSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESML
SEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVD
RAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQME
VYCSDFHAARAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQS
RHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQ
IHVMDCII*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4651
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.