cDNA Clone ERN1

• Plasmid: pFBHTa ERN1
• Parent Plasmid: pSC-A
• Expressed: His-ERN1
• Genbank: NM_001433
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGATCCGGAATTCATGCCGGCCC
GGCGGCTGCTGCTGCTGCTGACGCTGCTGCTGCCCGGCCTCGGGATTTTT
GGAAGTACCAGCACAGTGACGCTTCCTGAAACCTTGTTGTTTGTGTCAAC
GCTGGATGGAAGTTTGCATGCTGTCAGCAAGAGGACAGGCTCAATCAAAT
GGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACATGTGGAAGAG
CCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTATACGCTTGGAAG
CAAGAATAATGAAGGCCTGACGAAACTTCCTTTTACCATCCCAGAATTGG
TGCAGGCATCCCCATGCCGAAGTTCAGATGGAATCCTCTACATGGGTAAA
AAGCAGGACATCTGGTATGTTATTGACCTCCTGACCGGAGAGAAGCAGCA
GACTTTGTCATCGGCCTTTGCAGATAGTCTCTGCCCATCAACCTCTCTTC
TGTATCTTGGGCGAACAGAATACACCATCACCATGTACGACACCAAAACC
CGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGCGGCCTCACTGCC
TGAGGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGTGATG
GGCTGGTGGTGACTGTGGACAGTGAATCTGGGGACGTCCTGTGGATCCAA
AACTACGCCTCCCCTGTGGTGGCCTTTTATGTCTGGCAGCGGGAGGGTCT
GAGGAAGGTGATGCACATCAATGTCGCTGTGGAGACCCTGCGCTATCTGA
CCTTCATGTCTGGGGAGGTGGGGCGCATCACAAAGTGGAAGTACCCGTTC
CCCAAGGAGACAGAGGCCAAGAGCAAGCTGACGCCCACTCTGTATGTTGG
GAAATACTCTACCAGCCTCTATGCCTCTCCCTCAATGGTACACGAGGGGG
TTGCTGTCGTGCCCCGCGGCAGCACACTTCCTTTGCTGGAAGGGCCCCAG
ACTGATGGCGTCACCATCGGGGACAAGGGGGAGTGTGTGATCACGCCCAG
CACGGACGTCAAGTTTGATCCCGGACTCAAAAGCAAGAACAAGCTCAACT
ACTTGAGGAATTACTGGCTTCTGATAGGACACCATGAAACCCCACTGTCT
GCGTCTACCAAGATGCTGGAGAGATTTCCCAACAATCTACCCAAACATCG
GGAAAATGTGATTCCTGCTGATTCAGAGAAAAAGAGCTTTGAGGAAGTTA
TCAACCTGGTTGACCAGACTTCAGAAAACGCACCTACCACCGTGTCTCGG
GATGTGGAGGAGAAGCCCGCCCATGCCCCTGCCCGGCCCGAGGCCCCCGT
GGACTCCATGCTTAAGGACATGGCTACCATCATCCTGAGCACCTTCCTGC
TGATTGGCTGGGTGGCCTTCATCATCACCTATCCCCTGAGCATGCATCAG
CAGCAGCAGCTCCAGCACCAGCAGTTCCAGAAGGAACTGGAGAAGATCCA
GCTCCTGCAGCAGCAGCAGCAGCAGCTGCCCTTCCACCCACCTGGAGACA
CGGCTCAGGACGGCGAGCTCCTGGACACGTCTGGCCCGTACTCAGAGAGC
TCGGGCACCAGCAGCCCCAGCACGTCCCCCAGGGCCTCCAACCACTCACT
CTGCTCCGGCAGCTCTGCCTCCAAGGCTGGCAGCAGCCCCTCCCTGGAAC
AAGACGATGGAGATGAGGAAACCAGCGTGGTGATAGTTGGGAAAATTTCC
TTCTGTCCCAAGGATGTCCTGGGCCATGGAGCTGAGGGCACAATTGTGTA
CCGGGGCATGTTTGACAACCGCGACGTGGCCGTGAAGAGGATCCTCCCCG
AGTGTTTTAGCTTCGCAGACCGTGAGGTCCAGCTGTTGCGAGAATCGGAT
GAGCACCCGAACGTGATCCGCTACTTCTGCACGGAGAAGGACCGGCAATT
CCAGTACATTGCCATCGAGCTGTGTGCAGCCACCCTGCAAGAGTATGTGG
AGCAGAAGGACTTTGCGCATCTCGGCCTGGAGCCCATCACCTTGCTGCAG
CAGACCACCTCGGGCCTGGCCCACCTCCACTCCCTCAACATCGTTCACAG
AGACCTAAAGCCACACAACATCCTCATATCCATGCCCAATGCACACGGCA
AGATCAAGGCCATGATCTCCGACTTTGGCCTCTGCAAGAAGCTGGCAGTG
GGCAGACACAGTTTCAGCCGCCGATCTGGGGTGCCTGGCACAGAAGGCTG
GATCGCTCCAGAGATGCTGAGCGAAGACTGTAAGGAGAACCCTACCTACA
CGGTGGACATCTTTTCTGCAGGCTGCGTCTTTTACTACGTCATCTCTGAG
GGCAGCCACCCTTTTGGCAAGTCCCTGCAGCGGCAGGCCAACATCCTCCT
GGGTGCCTGCAGCCTTGACTGCTTGCACCCAGAGAAGCACGAAGACGTCA
TTGCACGTGAATTGATAGAGAAGATGATTGCGATGGATCCTCAGAAACGC
CCCTCGGCGAAGCACGTGCTCAAACACCCGTTCTTCTGGAGCCTAGAGAA
GCAGCTCCAGTTCTTCCAGGACGTGAGCGACAGAATAGAAAAGGAATCCC
TGGATGGCCCGATCGTGAAGCAGTTAGAGAGAGGCGGGAGAGCCGTGGTG
AAGATGGACTGGCGGGAGAACATCACTGACCCCCTCCAGACAGACCTGCG
TAAATTCAGGACCTATAAAGGTGGTTCTGTCAGAGATCTCCTCCGAGCCA
TGAGAAATAAGAAGCACCACTACCGGGAGCTGCCTGCAGAGGTGCGGGAG
ACGCTGGGGACCCTCCCCGACGACTTCGTGTGCTACTTCACATCTCGCTT
CCCCCACCTCCTCGCACACACCTACCGGGCCATGGAGCTGTGCAGCCACG
AGAGACTCTTCCAGCCCTACTACTTCCACGAGCCCCCAGAGCCCCAGCCC
CCAGTGACTCCAGACGCCCTCTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMPARRLLLLLTLLLPGLGIF
GSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEE
PAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGK
KQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKT
RELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQ
NYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPF
PKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQ
TDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLS
ASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSR
DVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQ
QQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSES
SGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKIS
FCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESD
EHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQ
QTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAV
GRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISE
GSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKR
PSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVV
KMDWRENITDPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRE
TLGTLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQP
PVTPDAL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4640
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H. Has 2 SNPS: V880D S924T - seen in other published sequences
• Low Temp: No