cDNA Clone Optineurin

• Plasmid: pCMV5-FLAG-1 Optineurin D474N
• Parent Plasmid: pCMV5
• Expressed: FLAG-Optineurin D474N
• Genbank: AF420371
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCATGTCCCATCA
ACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCACAG
GAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACCCCG
GAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCGAGAACCACCAGCT
GAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTGAGG
AGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTTGAG
ATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGAGAA
TGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAAGGT
CATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCGGAG
CAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGAGAA
GGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACTCCA
GCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGAGAA
GCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAGAAC
AGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAGATG
GGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTCCTG
CTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGCACT
CAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTAATC
GTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAATGAT
GAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAACCT
CCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAACTCA
GCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCCCTT
GAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGAGCT
TGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTATCAG
AAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTAACT
GTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGCATT
GAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACAGGG
CAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCTCTG
GCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAAGCA
GGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAGTTT
ACTGTTCTAATTTTCATGCTGAAAGAGCAGCGAGAGAGAAAATTCATGAG
GAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAATGA
TGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTCGTC
ATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAAAGA
GGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCATTC
CTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGATTC
ACGTGATGGATTGCATCATTTAAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEE
LLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQ
SKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQE
KDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAE
GSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLC
LREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEE
KGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALER
KNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVL
QMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALAS
KQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSNFHAERAAREKIHEEK
EQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGA
EDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4636
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG.