cDNA Clone Optineurin

• Plasmid: pEBG6P-1 Optineurin D474N
• Parent Plasmid: pEBG6P
• Expressed: GST-Optineurin D474N
• Genbank: AF420371
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAG
GACAGCCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACCC
AAACCTGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGC
TCCTGACCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAA
GCCATGAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGAA
GGAAGAACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTC
TAATGGCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAA
CTAAAAGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCAG
GCTTCCCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAGG
TGGTGAGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAA
CTGCAGCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTGA
AATTAGGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGC
ATAGTCCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAA
TATAGGAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGGA
GTTAACTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAGG
TGGAGAGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGAT
TTTGAAAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGGG
GAGCACAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAA
GCGAAGTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTT
CAAGAGGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGACT
TCAAGAAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCAG
AGTTGAATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCTA
CAAGTGGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAGA
GGATGAAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTC
TTCAAGAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAA
GAGTCAGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAACT
GGAACTGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAAA
TGAAGCAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCATC
CTCAGGGCTCAGATGGAAGTTTACTGTTCTAATTTTCATGCTGAAAGAGC
AGCGAGAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGG
CAGTTCTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCC
TTGATGGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACCA
GCAGGCTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAAC
AGCGGAATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCT
GACATAGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGCGGC
CGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSHQPLSCLTEKE
DSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQ
AMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGK
LKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSE
LQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSK
YRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSD
FEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKEL
QEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLEL
QVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRK
ESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTI
LRAQMEVYCSNFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQS
LMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLP
DIDTLQIHVMDCII*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4635
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.