cDNA Clone CDC25C

• Plasmid: pGEX6P-1-CDC25C
• Parent Plasmid: pGEX6P1
• Expressed: GST-CDC25C
• Genbank: P30307
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCTA
CGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCC
AGTTTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGA
CACTTCCTTTACCGTCTGTCCAGATGTCCCTAGAACTCCAGTGGGCAAAT
TTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGAACCCCAAAA
TGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACTGCCACTCA
GCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGATTCTTCAG
GACTTCAGGAAGTGCATTTAGCTGGGATGAATCATGACCAGCACCTAATG
AAATGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGGTTTGGACCG
TGGCCATAGAAAGAGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAAA
ATGACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTGGGCAGTCCC
ATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGGAGAAGACCA
GGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAGG
AAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAG
AACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACAC
AATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCA
GGAAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGACATTACTATC
ACTCAGATGCTGGAGGAAGATTCTAACCAGGGGCACCTGATTGGTGATTT
TTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAAGATCTGA
AGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCAG
GGTCTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTA
TCTGGGAGGACACATCCAGGGAGCCTTAAACTTATATAGTCAGGAAGAAC
TGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGACACCCAGAAG
AGAATAATCATCGTGTTCCACTGTGAATTCTCCTCAGAGAGGGGCCCCCG
AATGTGCCGCTGCCTGCGTGAAGAGGACAGGTCTCTGAACCAGTATCCTG
CATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTACAGAGACTTC
TTTCCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCA
TCATCAGGACCACAAGACTGAGTTGCTGAGGTGTCGAAGCCAGAGCAAAG
TGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTTCTGGTGAAG
GACATGAGCCCATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTELFSSTREEGSSGSGP
SFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPK
CCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLM
KCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEMKYLGSP
ITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPE
NLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITI
TQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQ
GLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIVPLDTQK
RIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDF
FPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVK
DMSP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4609
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.