cDNA Clone MEKK3

• Synonyms: MAP3K3
• Plasmid: pGEX6P-1-MEKK3 FL S526D
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEKK3 FL S526D
• Genbank: U78876
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGTGGCCCTC
CAGATGAACCGACGTCACCGGATGCCTGGATATGAGACCATGAAGAACAA
AGACACAGGTCACTCAAATAGGCAGAGTGACGTCAGAATCAAGTTCGAGC
ACAACGGGGAGAGGCGAATTATAGCGTTCAGCCGGCCTGTGAAATATGAA
GATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCTCTTGATCTACA
TTACATGAACAATGAGCTCTCCATCCTGCTGAAAAACCAAGATGATCTTG
ATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGG
ATATTGCTGTTGTCCCAGGACAGAAACCATAACAGTTCCTCTCCCCACTC
TGAGGTGTCCAGACAGGTGCGGATCAAGGCTTCCCAGTCCGCAGGGGATA
TAAATACTATCTACCAGCCCCCCGAGCCCAGAAGCAGGCACCTCTCTGTC
AGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGCTATGTTCCTGA
GCGGCAGCAGCACATTGCCCGGCAGGGGTCCTACACCAGCATCAACAGTG
AGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGATCCCCTG
AGCAGTGCAGAAAATTCCTTGTCTGGAAGCTGCCAATCCTTGGACAGGTC
AGCAGACAGCCCATCCTTCCGGAAATCACGAATGTCCCGTGCCCAGAGCT
TCCCTGACAACAGACAGGAATACTCAGATCGGGAAACTCAGCTTTATGAC
AAAGGGGTCAAAGGTGGAACCTACCCCCGGCGCTACCACGTGTCTGTGCA
CCACAAGGACTACAGTGATGGCAGAAGAACATTTCCCCGAATACGGCGTC
ATCAAGGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACA
AATGGCGAGAACATGGGCCTGGCTGTGCAATACCTGGACCCCCGTGGGCG
CCTGCGGAGTGCGGACAGCGAGAATGCCCTCTCTGTGCAGGAGAGGAATG
TGCCAACCAAGTCTCCCAGTGCCCCCATCAACTGGCGCCGGGGAAAGCTC
CTGGGCCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTATGACGTGGACAC
GGGACGTGAACTTGCTTCCAAGCAGGTCCAATTTGATCCAGACAGTCCTG
AGACAAGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAG
AACTTGCAGCATGAGCGCATCGTGCAGTACTATGGCTGTCTGCGGGACCG
CGCTGAGAAGACCCTGACCATCTTCATGGAGTACATGCCAGGGGGCTCGG
TGAAAGACCAGTTGAAGGCTTACGGTGCTCTGACAGAGAGCGTGACCCGA
AAGTACACGCGGCAGATCCTGGAGGGCATGTCCTACCTGCACAGCAACAT
GATTGTTCACCGGGACATTAAGGGAGCCAACATCCTCCGAGACTCTGCTG
GGAATGTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATC
TGTATGTCGGGGACGGGCATGCGCGACGTCACTGGCACACCCTACTGGAT
GAGCCCTGAGGTGATCAGCGGCGAGGGCTATGGAAGGAAAGCAGACGTGT
GGAGCCTGGGCTGCACTGTGGTGGAGATGCTGACAGAGAAACCACCGTGG
GCAGAGTATGAAGCTATGGCCGCCATCTTCAAGATTGCCACCCAGCCCAC
CAATCCTCAGCTGCCCTCCCACATCTCTGAACATGGCCGGGACTTCCTGA
GGCGCATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTC
ACACACCACTTTGCACAGCTCATGTACTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDEQEALNSIMNDLVAL
QMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYE
DVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLR
ILLLSQDRNHNSSSPHSEVSRQVRIKASQSAGDINTIYQPPEPRSRHLSV
SSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPL
SSAENSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYD
KGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLST
NGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKL
LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLK
NLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTR
KYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTI
CMSGTGMRDVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPW
AEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELL
THHFAQLMY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4607
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.