cDNA Clone MEKK3

• Synonyms: MAP3K3
• Plasmid: pGEX6P-1-MEKK3 delta 1-323 S526D
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEKK3 delta 1-323 S526D
• Genbank: U78876
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCTGGCTG
TGCAATACCTGGACCCCCGTGGGCGCCTGCGGAGTGCGGACAGCGAGAAT
GCCCTCTCTGTGCAGGAGAGGAATGTGCCAACCAAGTCTCCCAGTGCCCC
CATCAACTGGCGCCGGGGAAAGCTCCTGGGCCAGGGTGCCTTCGGCAGGG
TCTATTTGTGCTATGACGTGGACACGGGACGTGAACTTGCTTCCAAGCAG
GTCCAATTTGATCCAGACAGTCCTGAGACAAGCAAGGAGGTGAGTGCTCT
GGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAGCATGAGCGCATCGTGC
AGTACTATGGCTGTCTGCGGGACCGCGCTGAGAAGACCCTGACCATCTTC
ATGGAGTACATGCCAGGGGGCTCGGTGAAAGACCAGTTGAAGGCTTACGG
TGCTCTGACAGAGAGCGTGACCCGAAAGTACACGCGGCAGATCCTGGAGG
GCATGTCCTACCTGCACAGCAACATGATTGTTCACCGGGACATTAAGGGA
GCCAACATCCTCCGAGACTCTGCTGGGAATGTAAAGCTGGGGGACTTTGG
GGCCAGCAAACGCCTGCAGACGATCTGTATGTCGGGGACGGGCATGCGCG
ACGTCACTGGCACACCCTACTGGATGAGCCCTGAGGTGATCAGCGGCGAG
GGCTATGGAAGGAAAGCAGACGTGTGGAGCCTGGGCTGCACTGTGGTGGA
GATGCTGACAGAGAAACCACCGTGGGCAGAGTATGAAGCTATGGCCGCCA
TCTTCAAGATTGCCACCCAGCCCACCAATCCTCAGCTGCCCTCCCACATC
TCTGAACATGGCCGGGACTTCCTGAGGCGCATTTTTGTGGAGGCTCGCCA
GAGACCTTCAGCTGAGGAGCTGCTCACACACCACTTTGCACAGCTCATGT
ACTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLAVQYLDPRGRLRSADSEN
ALSVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQ
VQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIF
MEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKG
ANILRDSAGNVKLGDFGASKRLQTICMSGTGMRDVTGTPYWMSPEVISGE
GYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHI
SEHGRDFLRRIFVEARQRPSAEELLTHHFAQLMY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4606
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.