cDNA Clone EPHB1

• Plasmid: pGEX6P-1-EPHB1 565-984
• Parent Plasmid: pGEX6P1
• Expressed: GST-EPHB1 565-984
• Genbank: NM_004441
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCAGGAAACGGGCTTATAGCAAAGAGGCTGTGTACAGCGATAAGCTCCAG
CATTACAGCACAGGCCGAGGCTCCCCAGGGATGAAGATCTACATTGACCC
CTTCACTTACGAGGATCCCAACGAAGCTGTCCGGGAGTTTGCCAAGGAGA
TTGATGTATCTTTTGTGAAAATTGAAGAGGTCATCGGAGCAGGGGAGTTT
GGAGAAGTGTACAAGGGGCGTTTGAAACTGCCAGGCAAGAGGGAAATCTA
CGTGGCCATCAAGACCCTGAAGGCAGGGTACTCGGAGAAGCAGCGTCGGG
ACTTTCTGAGTGAGGCGAGCATCATGGGCCAGTTCGACCATCCTAACATC
ATTCGCCTGGAGGGTGTGGTCACCAAGAGTCGGCCTGTCATGATCATCAC
AGAGTTCATGGAGAATGGTGCATTGGATTCTTTCCTCAGGCAAAATGACG
GGCAGTTCACCGTGATCCAGCTTGTGGGTATGCTCAGGGGCATCGCTGCT
GGCATGAAGTACCTGGCTGAGATGAATTATGTGCATCGGGACCTGGCTGC
TAGGAACATTCTGGTCAACAGTAACCTGGTGTGCAAGGTGTCCGACTTTG
GCCTCTCCCGCTACCTCCAGGATGACACCTCAGATCCCACCTACACCAGC
TCCTTGGGAGGGAAGATCCCTGTGAGATGGACAGCTCCAGAGGCCATCGC
CTACCGCAAGTTCACTTCAGCCAGCGACGTTTGGAGCTATGGGATCGTCA
TGTGGGAAGTCATGTCATTTGGAGAGAGACCCTATTGGGATATGTCCAAC
CAAGATGTCATCAATGCCATCGAGCAGGACTACCGGCTGCCCCCACCCAT
GGACTGTCCAGCTGCTCTACACCAGCTCATGCTGGACTGTTGGCAGAAGG
ACCGGAACAGCCGGCCCCGGTTTGCGGAGATTGTCAACACCCTAGATAAG
ATGATCCGGAACCCGGCAAGTCTCAAGACTGTGGCAACCATCACCGCCGT
GCCTTCCCAGCCCCTGCTCGACCGCTCCATCCCAGACTTCACGGCCTTTA
CCACCGTGGATGACTGGCTCAGCGCCATCAAAATGGTCCAGTACAGGGAC
AGCTTCCTCACTGCTGGCTTCACCTCCCTCCAGCTGGTCACCCAGATGAC
ATCAGAAGACCTCCTGAGAATAGGCATCACCTTGGCAGGCCATCAGAAGA
AGATCCTGAACAGCATTCATTCTATGAGGGTCCAGATAAGTCAGTCACCA
ACGGCAATGGCATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFRKRAYSKEAVYSDKLQ
HYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEF
GEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNI
IRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAA
GMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTS
SLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSN
QDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDK
MIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRD
SFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSP
TAMA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4601
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST tag.