cDNA Clone CDC25C

• Plasmid: pEBG6P-1 CDC25C
• Parent Plasmid: pEBG6P
• Expressed: GST-CDC25C
• Genbank: P30307
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGA
AGCTCTGGCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAATGTTAAA
CCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCTA
GAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTG
TCTGGAGGAACCCCAAAATGTTGCCTCGATCTTTCGAATCTTAGCAGTGG
GGAGATAACTGCCACTCAGCTTACCACTTCTGCAGACCTTGATGAAACTG
GTCACCTGGATTCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGATGAAT
CATGACCAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGCAC
TCCGAATGGTTTGGACCGTGGCCATAGAAAGAGAGATGCAATGTGTAGTT
CATCTGCAAATAAAGAAAATGACAATGGAAACTTGGTGGACAGTGAAATG
AAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCC
AAACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGT
TTTCCCTGAAAGATCAGGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGC
TCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGA
AAAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTT
CTGGCCAAGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAAGACAGTCTCT
CTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGG
GCACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAG
GGAAACACCAAGATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTA
CTGTCGGGGAAGTTCCAGGGTCTGATTGAGAAGTTTTATGTCATTGATTG
TCGCTATCCATATGAGTATCTGGGAGGACACATCCAGGGAGCCTTAAACT
TATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTC
CCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTC
CTCAGAGAGGGGCCCCCGAATGTGCCGCTGCCTGCGTGAAGAGGACAGGT
CTCTGAACCAGTATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAA
GGCGGCTACAGAGACTTCTTTCCAGAATATATGGAACTGTGTGAACCACA
GAGCTACTGCCCTATGCATCATCAGGACCACAAGACTGAGTTGCTGAGGT
GTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAG
ATTGCCCTTCTGGTGAAGGACATGAGCCCATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSTELFSSTREEG
SSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSIL
SGGTPKCCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMN
HDQHLMKCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEM
KYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYR
SPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVS
LCDITITQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAAL
LSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIV
PLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILK
GGYRDFFPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQ
IALLVKDMSP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4600
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST tag.