cDNA Clone CDC25C

• Plasmid: pET28a(+) CDC25C
• Parent Plasmid: pET28a original
• Expressed: His-CDC25C
• Genbank: P30307
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCATGGTGCCGCG
CGGAAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGC
TCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCT
GGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCTAGAACTCCAG
TGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGA
ACCCCAAAATGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAAC
TGCCACTCAGCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGG
ATTCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGATGAATCATGACCAG
CACCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGG
TTTGGACCGTGGCCATAGAAAGAGAGATGCAATGTGTAGTTCATCTGCAA
ATAAAGAAAATGACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTG
GGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGG
AGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGA
AAGATCAGGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCG
ATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAA
GGACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAG
GAAAGCTCAGGAAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGAC
ATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGGCACCTGAT
TGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACC
AAGATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGG
AAGTTCCAGGGTCTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCC
ATATGAGTATCTGGGAGGACACATCCAGGGAGCCTTAAACTTATATAGTC
AGGAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGAC
ACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTCCTCAGAGAG
GGGCCCCCGAATGTGCCGCTGCCTGCGTGAAGAGGACAGGTCTCTGAACC
AGTATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTAC
AGAGACTTCTTTCCAGAATATATGGAACTGTGTGAACCACAGAGCTACTG
CCCTATGCATCATCAGGACCACAAGACTGAGTTGCTGAGGTGTCGAAGCC
AGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTT
CTGGTGAAGGACATGAGCCCATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGMVPRGSHMASMTGGQQMGRGSMSTELFSSTREEGSSG
SGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGG
TPKCCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQ
HLMKCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEMKYL
GSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPS
MPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCD
ITITQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSG
KFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIVPLD
TQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGY
RDFFPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIAL
LVKDMSP*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU4599
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term 6H tag.