cDNA Clone JAK1

• Plasmid: pFBHTc JAK1
• Parent Plasmid: pFastbac HTc
• Expressed: His-JAK1
• Genbank: NM_002227
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGGATCCGGAATTCAAAGGCCT
ACGTCGACATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACTGAG
GTGAACCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTTCTATCTGTC
GGACAGGGAGCCCCTCCGGCTGGGCAGTGGAGAGTACACAGCAGAGGAAC
TGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCTCTTTGTCACAAC
CTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCAAATCG
CACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCGGATGA
GGTTCTATTTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCAGTG
TGGCGTCATTCTCCAAAGAAGCAGAAAAATGGCTACGAGAAAAAAAAGAT
TCCAGATGCAACCCCTCTCCTTGATGCCAGCTCACTGGAGTATCTGTTTG
CTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGACCCC
AAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGGATGGC
TGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGCCAG
AACTGCCCAAGGACATCAGCTACAAGCGATATATTCCAGAAACATTGAAT
AAGTCCATCAGACAGAGGAACCTTCTCACCAGGATGCGGATAAATAATGT
TTTCAAGGATTTCCTAAAGGAATTTAACAACAAGACCATTTGTGACAGCA
GCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCTACCTTGGAAACT
TTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTACTGAT
TTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGGAAACG
TTCTCTACTACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGTGGAGG
CATAAACCAAATGTTGTTTCTGTTGAAAAGGAAAAAAATAAACTGAAGCG
GAAAAAACTGGAAAATAAAGACAAGAAGGATGAGGAGAAAAACAAGATCC
GGGAAGAGTGGAACAATTTTTCATTCTTCCCTGAAATCACTCACATTGTA
ATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAAATGGA
ACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCTGGTAG
ATGGCTACTTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCACCGAC
GTGGCCCCCCCGTTGATCGTCCACAACATACAGAATGGCTGTCATGGTCC
AATCTGTACAGAATACGCCATCAATAAATTGCGGCAAGAAGGAAGCGAGG
AGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTTGACAACATCCTC
ATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCAGAA
GCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAGTCTGC
ACGGTTCGGACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCCACCTC
AAGAAGCAGATCCTGCGCACGGATAACATCAGCTTCATGCTAAAACGCTG
CTGCCAGCCCAAGCCCCGAGAAATCTCCAACCTGCTGGTGGCTACTAAGA
AAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAGCTGAGTTTCGAT
CGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGGCAC
GAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGACGAAG
GAACTTCTGAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAGACCCC
AGCCACAGGGATATTTCCCTGGCCTTCTTCGAGGCAGCCAGCATGATGAG
ACAGGTCTCCCACAAACACATCGTGTACCTCTATGGCGTCTGTGTCCGCG
ACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCCTCTGGAT
CTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATGGAAATTCAA
AGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAAAGACC
TGGTCCATGGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGC
ATCGACAGTGAGTGTGGCCCATTCATCAAGCTCAGTGACCCCGGCATCCC
CATTACGGTGCTGTCTAGGCAAGAATGCATTGAACGAATCCCATGGATTG
CTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTGGCTGCTGACAAG
TGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAGATCCC
CTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAGCCGGT
GCAGGCCAGTGACACCATCATGTAAGGAGCTGGCTGACCTCATGACCCGC
TGCATGAACTATGACCCCAATCAGAGGCCTTTCTTCCGAGCCATCATGAG
AGACATTAATAAGCTTGAAGAGCAGAATCCAGATATTGTTTCCAGAAAAA
AAAACCAGCCAACTGAAGTGGACCCCACACATTTTGAAAAGCGCTTCCTA
AAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAGCTCTG
CAGGTATGACCCCGAAGACAATACAGGGGAGCAGGTGGCTGTTAAATCTC
TGAAGCCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGGAAATC
GAGATCTTAAGGAACCTCTATCATGAGAACATTGTGAAGTACAAAGGAAT
CTGCACAGAAGACGGAGGAAATGGTATTAAGCTCATCATGGAATTTCTGC
CTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAGAACAAAATAAAC
CTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATGGACTA
TTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAATGTCC
TTGTTGAGAGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAACCAAA
GCAATTGAAACCGATAAGGAGTATTACACCGTCAAGGATGACCGGGACAG
CCCTGTGTTTTGGTATGCTCCAGAATGTTTAATGCAATCTAAATTTTATA
TTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCATGAGCTGCTGACT
TACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATGATAGG
CCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTTAAAAG
AAGGAAAACGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTATCAA
CTTATGAGGAAATGCTGGGAATTCCAACCATCCAATCGGACAAGCTTTCA
GAACCTTATTGAAGGATTTGAAGCACTTTTAAAATAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDMAFCAKMRSSKKTE
VNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHN
LFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSV
WRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDP
KTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLN
KSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLET
LTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWR
HKPNVVSVEKEKNKLKRKKLENKDKKDEEKNKIREEWNNFSFFPEITHIV
IKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTD
VAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNIL
MTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHL
KKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFD
RILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDP
SHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLD
LFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREG
IDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADK
WSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTR
CMNYDPNQRPFFRAIMRDINKLEEQNPDIVSRKKNQPTEVDPTHFEKRFL
KRIRDLGEGHFGKVELCRYDPEDNTGEQVAVKSLKPESGGNHIADLKKEI
EILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKIN
LKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTK
AIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLT
YCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQ
LMRKCWEFQPSNRTSFQNLIEGFEALLK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4557
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H.