cDNA Clone JAK1

• Plasmid: pGEX6P-3-JAK1
• Parent Plasmid: pGEX6P3
• Expressed: GST-JAK1
• Genbank: NM_002227
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATT
CCCGGGTCGACATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACT
GAGGTGAACCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTTCTATCT
GTCGGACAGGGAGCCCCTCCGGCTGGGCAGTGGAGAGTACACAGCAGAGG
AACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCTCTTTGTCAC
AACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCAAA
TCGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCGGA
TGAGGTTCTATTTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCA
GTGTGGCGTCATTCTCCAAAGAAGCAGAAAAATGGCTACGAGAAAAAAAA
GATTCCAGATGCAACCCCTCTCCTTGATGCCAGCTCACTGGAGTATCTGT
TTGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGAC
CCCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGGAT
GGCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGC
CAGAACTGCCCAAGGACATCAGCTACAAGCGATATATTCCAGAAACATTG
AATAAGTCCATCAGACAGAGGAACCTTCTCACCAGGATGCGGATAAATAA
TGTTTTCAAGGATTTCCTAAAGGAATTTAACAACAAGACCATTTGTGACA
GCAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCTACCTTGGAA
ACTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTACT
GATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGGAA
ACGTTCTCTACTACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGTGG
AGGCATAAACCAAATGTTGTTTCTGTTGAAAAGGAAAAAAATAAACTGAA
GCGGAAAAAACTGGAAAATAAAGACAAGAAGGATGAGGAGAAAAACAAGA
TCCGGGAAGAGTGGAACAATTTTTCATTCTTCCCTGAAATCACTCACATT
GTAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAAAT
GGAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCTGG
TAGATGGCTACTTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCACC
GACGTGGCCCCCCCGTTGATCGTCCACAACATACAGAATGGCTGTCATGG
TCCAATCTGTACAGAATACGCCATCAATAAATTGCGGCAAGAAGGAAGCG
AGGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTTGACAACATC
CTCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCA
GAAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAGTC
TGCACGGTTCGGACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCCAC
CTCAAGAAGCAGATCCTGCGCACGGATAACATCAGCTTCATGCTAAAACG
CTGCTGCCAGCCCAAGCCCCGAGAAATCTCCAACCTGCTGGTGGCTACTA
AGAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAGCTGAGTTTC
GATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGG
CACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGACG
AAGGAACTTCTGAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAGAC
CCCAGCCACAGGGATATTTCCCTGGCCTTCTTCGAGGCAGCCAGCATGAT
GAGACAGGTCTCCCACAAACACATCGTGTACCTCTATGGCGTCTGTGTCC
GCGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCCTCTG
GATCTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATGGAAATT
CAAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAAAG
ACCTGGTCCATGGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTGAG
GGCATCGACAGTGAGTGTGGCCCATTCATCAAGCTCAGTGACCCCGGCAT
CCCCATTACGGTGCTGTCTAGGCAAGAATGCATTGAACGAATCCCATGGA
TTGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTGGCTGCTGAC
AAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAGAT
CCCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAGCC
GGTGCAGGCCAGTGACACCATCATGTAAGGAGCTGGCTGACCTCATGACC
CGCTGCATGAACTATGACCCCAATCAGAGGCCTTTCTTCCGAGCCATCAT
GAGAGACATTAATAAGCTTGAAGAGCAGAATCCAGATATTGTTTCCAGAA
AAAAAAACCAGCCAACTGAAGTGGACCCCACACATTTTGAAAAGCGCTTC
CTAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAGCT
CTGCAGGTATGACCCCGAAGACAATACAGGGGAGCAGGTGGCTGTTAAAT
CTCTGAAGCCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGGAA
ATCGAGATCTTAAGGAACCTCTATCATGAGAACATTGTGAAGTACAAAGG
AATCTGCACAGAAGACGGAGGAAATGGTATTAAGCTCATCATGGAATTTC
TGCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAGAACAAAATA
AACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATGGA
CTATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAATG
TCCTTGTTGAGAGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAACC
AAAGCAATTGAAACCGATAAGGAGTATTACACCGTCAAGGATGACCGGGA
CAGCCCTGTGTTTTGGTATGCTCCAGAATGTTTAATGCAATCTAAATTTT
ATATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCATGAGCTGCTG
ACTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATGAT
AGGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTTAA
AAGAAGGAAAACGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTAT
CAACTTATGAGGAAATGCTGGGAATTCCAACCATCCAATCGGACAAGCTT
TCAGAACCTTATTGAAGGATTTGAAGCACTTTTAAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMAFCAKMRSSKKT
EVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCH
NLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQS
VWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRD
PKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETL
NKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLE
TLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQW
RHKPNVVSVEKEKNKLKRKKLENKDKKDEEKNKIREEWNNFSFFPEITHI
VIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCT
DVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNI
LMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSH
LKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSF
DRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLD
PSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPL
DLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLARE
GIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAAD
KWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMT
RCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSRKKNQPTEVDPTHFEKRF
LKRIRDLGEGHFGKVELCRYDPEDNTGEQVAVKSLKPESGGNHIADLKKE
IEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKI
NLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLT
KAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELL
TYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVY
QLMRKCWEFQPSNRTSFQNLIEGFEALLK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4540
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.