cDNA Clone JAK1

• Plasmid: pCMV5-FLAG-3 JAK1
• Parent Plasmid: pCMV5
• Expressed: FLAG-JAK1
• Genbank: NM_002227
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGGGATCCCCGGAATTC
CCGGGTCGACATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACTG
AGGTGAACCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTTCTATCTG
TCGGACAGGGAGCCCCTCCGGCTGGGCAGTGGAGAGTACACAGCAGAGGA
ACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCTCTTTGTCACA
ACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCAAAT
CGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCGGAT
GAGGTTCTATTTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCAG
TGTGGCGTCATTCTCCAAAGAAGCAGAAAAATGGCTACGAGAAAAAAAAG
ATTCCAGATGCAACCCCTCTCCTTGATGCCAGCTCACTGGAGTATCTGTT
TGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGACC
CCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGGATG
GCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGCC
AGAACTGCCCAAGGACATCAGCTACAAGCGATATATTCCAGAAACATTGA
ATAAGTCCATCAGACAGAGGAACCTTCTCACCAGGATGCGGATAAATAAT
GTTTTCAAGGATTTCCTAAAGGAATTTAACAACAAGACCATTTGTGACAG
CAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCTACCTTGGAAA
CTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTACTG
ATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGGAAA
CGTTCTCTACTACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGTGGA
GGCATAAACCAAATGTTGTTTCTGTTGAAAAGGAAAAAAATAAACTGAAG
CGGAAAAAACTGGAAAATAAAGACAAGAAGGATGAGGAGAAAAACAAGAT
CCGGGAAGAGTGGAACAATTTTTCATTCTTCCCTGAAATCACTCACATTG
TAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAAATG
GAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCTGGT
AGATGGCTACTTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCACCG
ACGTGGCCCCCCCGTTGATCGTCCACAACATACAGAATGGCTGTCATGGT
CCAATCTGTACAGAATACGCCATCAATAAATTGCGGCAAGAAGGAAGCGA
GGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTTGACAACATCC
TCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCAG
AAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAGTCT
GCACGGTTCGGACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCCACC
TCAAGAAGCAGATCCTGCGCACGGATAACATCAGCTTCATGCTAAAACGC
TGCTGCCAGCCCAAGCCCCGAGAAATCTCCAACCTGCTGGTGGCTACTAA
GAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAGCTGAGTTTCG
ATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGGC
ACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGACGA
AGGAACTTCTGAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAGACC
CCAGCCACAGGGATATTTCCCTGGCCTTCTTCGAGGCAGCCAGCATGATG
AGACAGGTCTCCCACAAACACATCGTGTACCTCTATGGCGTCTGTGTCCG
CGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCCTCTGG
ATCTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATGGAAATTC
AAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAAAGA
CCTGGTCCATGGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGG
GCATCGACAGTGAGTGTGGCCCATTCATCAAGCTCAGTGACCCCGGCATC
CCCATTACGGTGCTGTCTAGGCAAGAATGCATTGAACGAATCCCATGGAT
TGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTGGCTGCTGACA
AGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAGATC
CCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAGCCG
GTGCAGGCCAGTGACACCATCATGTAAGGAGCTGGCTGACCTCATGACCC
GCTGCATGAACTATGACCCCAATCAGAGGCCTTTCTTCCGAGCCATCATG
AGAGACATTAATAAGCTTGAAGAGCAGAATCCAGATATTGTTTCCAGAAA
AAAAAACCAGCCAACTGAAGTGGACCCCACACATTTTGAAAAGCGCTTCC
TAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAGCTC
TGCAGGTATGACCCCGAAGACAATACAGGGGAGCAGGTGGCTGTTAAATC
TCTGAAGCCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGGAAA
TCGAGATCTTAAGGAACCTCTATCATGAGAACATTGTGAAGTACAAAGGA
ATCTGCACAGAAGACGGAGGAAATGGTATTAAGCTCATCATGGAATTTCT
GCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAGAACAAAATAA
ACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATGGAC
TATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAATGT
CCTTGTTGAGAGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAACCA
AAGCAATTGAAACCGATAAGGAGTATTACACCGTCAAGGATGACCGGGAC
AGCCCTGTGTTTTGGTATGCTCCAGAATGTTTAATGCAATCTAAATTTTA
TATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCATGAGCTGCTGA
CTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATGATA
GGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTTAAA
AGAAGGAAAACGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTATC
AACTTATGAGGAAATGCTGGGAATTCCAACCATCCAATCGGACAAGCTTT
CAGAACCTTATTGAAGGATTTGAAGCACTTTTAAAATAAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSD
REPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRT
ITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIP
DATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAV
LAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVF
KDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLIS
SENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRK
KLENKDKKDEEKNKIREEWNNFSFFPEITHIVIKESVVSINKQDNKKMEL
KLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPI
CTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQ
FKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCC
QPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTR
THIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQ
VSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKV
AKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPI
TVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPL
KDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRD
INKLEEQNPDIVSRKKNQPTEVDPTHFEKRFLKRIRDLGEGHFGKVELCR
YDPEDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGIC
TEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYL
GSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSP
VFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGP
THGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQN
LIEGFEALLK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4539
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG.